Simultaneous determination of tryptophan and kynurenine in serum by HPLC with UV and fluorescence detection

Biomedical Chromatography

Volumn 18, Issue 10, 2004, Pages 872-874

  Vignau, J ^a   Jacquemont, M C ^b   Lefort, A ^b   Imbenotte, M ^b   Lhermitte, M ^b  

^a LILLE UNIVERSITY HOSPITAL   (France)

^b UNIV LILLE   (France)

Author keywords

Blood serum; HPLC; Indoleamine 2,3 dioxygenase (IDO); Kynurenine production; Mental depression; Tryptophan

Indexed keywords

AMINO ACIDS; BLOOD;

BLOOD SERUM; DIOXYGENASES; HPLC; INDOLEAMINE 2,3 DIOXYGENASE; INDOLEAMINES; KYNURENINE PRODUCTION; MENTAL DEPRESSION; SIMULTANEOUS DETERMINATIONS; TRYPTOPHAN; UV DETECTION;

INTERFERONS;

INDOLEAMINE 2,3 DIOXYGENASE; INTERFERON; KYNURENINE; TRYPTOPHAN;

AMINO ACID ANALYSIS; AMINO ACID BLOOD LEVEL; AMINO ACID SYNTHESIS; ARTICLE; BLOOD SAMPLING; CALIBRATION; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONTROLLED STUDY; DEPRESSION; ENZYME INDUCTION; FLUORESCENCE ANALYSIS; HEPATITIS C; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOTHERAPY; LINEAR SYSTEM; QUANTITATIVE ANALYSIS; REGRESSION ANALYSIS; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TRYPTOPHAN METABOLISM; ULTRAVIOLET RADIATION; VALIDATION PROCESS;

HEPATITIS C VIRUS;

EID: 11844304301     PISSN: 02693879     EISSN: None     Source Type: Journal    
DOI: 10.1002/bmc.445     Document Type: Article

References (6)

1. Bonaccorso S, Marino V, Puzella A, Pasquini. M, Biondi M, Artini M, Almerighi C, Verkerk R, Meltzer H and Maes M. Increased depressive ratings in patients with hepatitis C receiving interferon-α-based immunotherapy are related to interferon-α -induced changes in the serotonergic system. Journal of Clinical Psychopharmacology 2002; 22: 86-89.
2. Gad S. Statistics and Experimental Design for Toxicologists. CRC Press: Boca Raton, FL, 1999; 110.
3. Hervé C, Beyne P, Jamault H and Delacoux E. Determination of tryptophan and its kynurenine pathway metabolites in human serum by high-performance liquid chromatography with simultaneous ultraviolet and fluorimetric detection. Journal of Chromatography B 1996; 675 157-161.
4. Holmes EW. Determination of serum kynurenine and hepatic tryptophan dioxygenase activity by high-performance liquid chromatography. Analytical Biochemistry 1988; 172: 518-525.
5. Laich A, Neurater G, Widner B and Fuchs D. More rapid method for simultaneous measurement of tryptophan and kynurenine by HPLC. Clinical Chemistry 2002; 48: 579-581.
6. Widner B, Werner ER, Schermach H and Fuchs D. An HPLC method to determine tryptophan and kynurenine in serum simultaneously. Advances in Experimental Medicine and Biology 1999; 467: 87-32.