Long-term lens organ culture system to determine age-related effects of UV irradiation on the eye lens

Experimental Eye Research

Volumn 79, Issue 6, 2004, Pages 903-911

  Azzam, Naiel ^a   Dovrat, Ahuva ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

aging; eye lens; lens enzymes; organ culture; UV A

Indexed keywords

AGING; ANIMAL TISSUE; CATARACTOGENESIS; CELL CULTURE; CONFERENCE PAPER; CONTROLLED STUDY; ENVIRONMENTAL FACTOR; ENZYME ANALYSIS; EPITHELIUM; INCUBATION TIME; LENS; LENS DISEASE; MALE; NONHUMAN; ORGAN CULTURE; PRIORITY JOURNAL; SOLAR RADIATION; ULTRAVIOLET A RADIATION; ULTRAVIOLET IRRADIATION;

AGING; ANIMALS; CATTLE; DOSE FRACTIONATION; LENS, CRYSTALLINE; MALE; OPTICS; ORGAN CULTURE TECHNIQUES; RADIATION INJURIES; REGENERATION; TIME FACTORS; ULTRAVIOLET RAYS;

EID: 11844286388     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2004.06.021     Document Type: Conference Paper

References (33)

1. R.F. Beers, and I.W. Sizer A spectophotometric methods for measuring the breakdown of hydrogen peroxide by catalase J. Biol. Chem. 195 1952 133 140
2. J.A. Carver, J.A. Aquilina, P.G. Cooper, G.A. Williams, and R.J. Truscott α-Crystallin: molecular chaperone and protein surfactant Biochim. Biophys. Acta 1204 1994 195 206
3. B.K. Derham, and J.J. Harding α-Crystallin as a molecular chaperone Prog. Retin. Eye Res. 18 1999 463 509
4. A. Dovrat, and D. Gershon Rat lens superoxide dismutase and glucose-6-phosphate dehydrogenase: studies on the catalytic activity and the fate of enzyme antigen as a function of age Exp. Eye Res. 33 1981 651 661
5. A. Dovrat, and D. Gershon Studies on the fate of aldolase molecules in the aging rat lens Biochim. Biophys. Acta 757 1983 164 167
6. A. Dovrat, J. Scharf, and D. Gershon Glyceraldehyde 3-phosphate dehydrogenase activity in rat and human lenses and the fate of enzyme molecules in the aging lens Mech. Ageing Dev. 28 1984 187 191
7. A. Dovrat, and O. Weinreb Recovery of lens optics and epithelial enzymes after utraviolet-A radiation Exp. Eye Res. 36 1995 2417 2424
8. J.W. Eaton UV-mediated cataractogenesis: a radical perspective Doc. Ophthalmol. 88 1995 233 242
9. E. Ganea, and J.J. Harding α-Crystallin protects glucose-6-phosphate dehydrogenase aginst inactivation by malondialdehyde Biochim. Biophys. Acta 1500 2000 49 58
10. H. Gershon, and D. Gershon Inactive enzyme molecules in aging mice; liver aldolase Proc. Natl Acad. Sci. USA 70 1973 909 913
11. F.J. Giblin, V.R. Leverenz, V.A. Padgaonkar, N.J. Unakar, L. Dang, L.R. Lin, M.F. Lou, V.N. Reddy, D. Borchman, and J.P. Dillon UVA light in vivo reaches the nucleus of the guinea pig lens and produces deleterious, oxidative effects Exp. Eye Res. 75 2002 445 458
12. K. Hightower, and J. McCready Comparative effect of UV-A and UV-B on cultured rabbit lens Photochem. Photobiol. 58 1993 827 830
13. O. Hockwin, M. Kojima, Y. Sakamoto, A. Wegener, Y.B. Shui, and K. Sasaki UV damage to the eye lens: further results from animal model studies: a review J. Epidemiol. 9 1999 S39 S47
14. D.W. Hook, and J.J. Harding Protection of enzymes by α-crystallin acting as a molecular chaperone Int. J. Biol. Macromol. 22 1998 295 306
15. J. Horwitz α-Crystallin can function as a molecular chaperone Proc. Natl Acad. Sci. USA 89 1992 10449 10453
16. O.M. Lardinois, M.M. Mestdagh, and P.G. Rouxhet Reversible inhibition and irreversible inactivation of catalase in presence of hydrogen peroxide Biochim. Biophys. Acta 1295 1996 222 238
17. K.W. Lee, N. Meyer, and B.J. Ortwerth Chromatographic comparison of the UVA sensitizers present in brunescent cataracts and in calf lens proteins ascorbylated in-vitro Exp. Eye Res. 69 1999 375 384
18. M. Linetsky, V.G. Chemoganskiy, F. Hu, and B.J. Ortwerth Effect of UVA light on the activity of several aged human lens enzymes Invest. Ophthalmol. Vis. Sci. 44 2003 264 274
19. M. Linetsky, and B.J. Ortwerth The generation of hydrogen peroxide by the UVA irradiation of human lens proteins Photchem. Photobiol. 62 1995 87 93
20. M. Linetsky, and B.J. Ortwerth Quantification of the reactive oxygen species generated by the UVA irradiation of ascorbic acid-glycated lens proteins Photochem. Photobiol. 63 1996 649 655
21. O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall Protein measurement with the Foline phenol reagent J. Biol. Chem. 193 1951 265 275
22. A. Mahns, I. Melchheier, C.V. Suschek, H. Sies, and L.O. Klotz Irradiation of cells with ultraviolet-A (320-400nm) in the presence of cell culture medium elicits biological effects due to extracellular generation of hydrogen peroxide Free Radic. Res. 37 2003 391 397
23. B.A. Moffat, K.A. Landman, R.J. Truscott, M.H. Sweeny, and J.M. Pope Age-related changes in the kinetics of water transport in normal human lenses Exp. Eye Res. 69 1999 663 669
24. P.V. Rao, Q.L. Huang, J. Horwitz, and J.S. Zigler Jr Evidence that α-crystallin prevents specific protein aggregation in the intact eye lens Biochim. Biophys. Acta 1245 1995 439 447
25. M.H. Sweeney, and R.J. Truscott An impediment to glutathione diffusion in older normal human lenses: a possible precondition for nuclear cataract Exp. Eye Res. 67 1998 587 595
26. H.R. Taylor The enviroment and the lens Br. J. Ophthalmol. 64 1980 303 310
27. O. Weinreb, M.A. Van Boekel, A. Dovrat, and H. Bloemendal Effect of UV-A light on the chaperone-like properties of young and old lens α-crystallin Invest. Ophthalmol. Vis. Sci. 41 2000 191 198
28. R.W. Young Sunlight and age-related eye disease J. Natl Med. Assoc. 84 1992 353 358
29. P.S. Zigman The role of sunlight in human cataract formation Surv. Ophthlmol. 27 1983 317 326
30. S. Zigman Recent research on near UV radiation and the eye F. Urbach R.W. Gange The Biological Effect of UV-A Radiation 1986 Praegner New York 252 262
31. S. Zigman, T. McDaniel, J.B. Schultz, J. Reddan, and M. Meydani Damage to cultured lens epithelial cells of squirrels and rabbits by UV-A (99.9%)+UV-B (0.1%) radiation and α-tocopherol protection Mol. Cell. Biochem. 143 1995 35 46
32. S. Zigman, J. Reddan, J.B. Schultz, and T. McDaniel Structural and functional changes in catalase induced by near-UV radiation Photochem. Photobiol. 63 1996 818 824
33. S. Zigman, J.B. Schultz, and M. Schultz Measurment of oxygen production by invitro human and animal lenses with an oxygen electrode Curr. Eye Res. 17 1998 115 119