Overexpression and purification of Pyrococcus abyssi phosphopantetheine adenylyltransferase from an optimized synthetic gene for NMR studies

Protein Expression and Purification

Volumn 39, Issue 2, 2005, Pages 296-306

  Nálezková, Monika ^a   De Groot, Arjan ^a   Graf, Marcus ^b   Gans, Pierre ^a   Blanchard, Laurence ^a  

^a INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^b GENEART GmbH   (Germany)

Author keywords

CoA biosynthesis; NMR; Optimized synthetic gene; PPAT; Protein overexpression

Indexed keywords

NUCLEOTIDYLTRANSFERASE; PANTETHEINE PHOSPHATE ADENYLYLTRANSFERASE; PANTETHEINE-PHOSPHATE ADENYLYLTRANSFERASE; PROTON; SYNTHETIC DNA;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; CODON; COMPARATIVE STUDY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; PYROCOCCUS ABYSSI; SEQUENCE HOMOLOGY; SOLUBILITY;

AMINO ACID SEQUENCE; CIRCULAR DICHROISM; CODON; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, SYNTHETIC; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; NUCLEOTIDYLTRANSFERASES; PLASMIDS; PROTEIN STRUCTURE, SECONDARY; PROTONS; PYROCOCCUS ABYSSI; SEQUENCE HOMOLOGY, AMINO ACID; SOLUBILITY; TRANSFORMATION, GENETIC;

ARCHAEA; ESCHERICHIA COLI; PYROCOCCUS ABYSSI;

EID: 11844271472     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.11.003     Document Type: Article

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