Protein damage in drop-on-demand printers

Journal of the American Chemical Society

Volumn 126, Issue 50, 2004, Pages 16320-16321

  Nishioka, Gary M ^a   Markey, Andrea A ^a   Holloway, Charles K ^a  

^a H and N Instruments Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AQUEOUS SOLUTION; ARTICLE; CALIBRATION; CHEMICAL PROCEDURES; ENZYME ACTIVITY; FLUORESCENCE; HYDROGEN BOND; ILLUMINATION; PROTEIN DEFECT; STATISTICAL SIGNIFICANCE;

ENZYME STABILITY; FLUORESCEIN; GLUCOSE; MICROARRAY ANALYSIS; PEROXIDASES; TREHALOSE;

EID: 11444265203     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja044539z     Document Type: Article

References (19)

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9. Added to each well is 200 μL of a solution consisting of 0.4 mg/mL of o-phenylenediamine dihydrochloride, 0.4 mg/mL of urea hydrogen peroxide, and 0.05 M phosphate/citrate buffer (Sigma, OPD tablet sets).
10. In a separate experiment, peroxidase solutions were discharged through the printhead aperture at linear flows up to 300 cm/s. No loss in activity was detected at these flow rates. Typical linear speeds used in inkjet printers are much higher, typically 10 m/s.
11. Control solutions are collected by flowing solution through the printhead under low pressure (∼30 psig). Activity of these solutions is identical to that of solutions collected from the sample reservoir. Thus, no loss in activity due to adsorption in the printhead and shearing at the orifice is observed.
12. Printing occurs under nonresonance conditions, at 100 Hz. An asymmetric waveform is used, consisting of compression (droplet ejection) over microseconds and expansion (refilling) over milliseconds.
13. -12 m/V, V = applied voltage, r = tube radius = 0.5 mm, and t = wall thickness = 0.6 mm.
14. Liquid flows in two directions as a result of compression. Liquid flows out of the orifice, forming the printed jet. It also flows through the inlet back into the solvent reservoir. The relative amount of flow in each direction depends on the compression rate.
15. This solution consists of 10 μg/mL of horseradish peroxidase, 10 μg/mL of fluorescein sodium salt, 10% (wt/vol) D-(+)trehalose, and 1% D-(+)-glucose in phosphate-buffered saline.
16. Sun, W. Q.; Davidson, P. Biochim. Biophys. Acta 1998, 1425, 235.
17. Beadle, B. M.; Shoichet, B. K. J. Mol. Biol. 2002, 321, 285.
18. Protein Stability and Storage; Pierce Technical Bulletin TR043.0.
19. Kaushik, J. K.; Bhat, R. J. Biol. Chem. 2003, 278, 26458.