Che a 1: Recombinant expression, purification and correspondence to the natural form

International Archives of Allergy and Immunology

Volumn 135, Issue 4, 2004, Pages 284-292

  Barderas, Rodrigo ^a   Villalba, Mayte ^a   Rodríguez, Rosalía ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Che a 1; Chenopodium album pollen; Ole e 1; Pichia pastoris; Recombinant allergen

Indexed keywords

ALLERGEN; CHE A 1; CONCANAVALIN A; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DEGRADATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; HUMAN; IMMUNOBLOTTING; ISOLATION PROCEDURE; MASS SPECTROMETRY; MOLECULAR WEIGHT; PICHIA PASTORIS; POLLEN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STAINING;

ALLERGENS; BLOTTING, WESTERN; CHENOPODIUM ALBUM; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; HYPERSENSITIVITY; IMMUNOGLOBULIN E; PICHIA; POLLEN; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 11444264766     PISSN: 10182438     EISSN: None     Source Type: Journal    
DOI: 10.1159/000082321     Document Type: Article

References (31)

1. Homan RB: Lamb's quarters pollen. Ann Allergy 1963;21:647-649.
2. Galán C, Infante F, Ruiz de Clavijo E, Guerra F, Miguel R, Domínguez E: Allergy to pollen grains from Amaranthaceae and Chenopodiaceae in Cordoba, Spain. Annual and daily variation of pollen concentration. Ann Allergy 1989;63:435-438.
3. Newmark FM: The hay fever plants of Colorado. Ann Allergy 1978;40:18-24.
4. Lombardero M, Duffort O, Carreira J: Allergenic significance of chenopod pollen; in D'Amato G, Spieksma FTM, Bonini S (eds): Allergenic Pollen and Pollinosis in Europe. Oxford, Blackwell, 1991, pp 128-131.
5. Suliaman FA, Holmes WF, Kwick S, Khouri F, Ratard R: Pattern of immediate type hypersensitivity reactions in the Eastern Province, Saudi Arabia. Ann Allergy Asthma Immunol 1997;78:415-418.
6. Ezeamuzie CI, Thomson MS, Al-Ali S, Dowaisan A, Khan M, Hijazi Z: Asthma in the desert: Spectrum of the sensitizing aeroallergens. Allergy 2000;55:157-162.
7. Al-Dowaisan A, Fakim N, Khan MR, Arifhodzic N, Panicker R, Hanoon A, Khan I: Salsola pollen as a predominant cause of respiratory allergies in Kuwait. Ann Allergy Asthma Immunol 2004;92:262-267.
8. Ezeamuzie CI, al-Mousawi M, Dashti H, al-Bashir A, al-Hage M, al-Ali S: Prevalence of allergic sensitization to inhalant allergens among blood donors in Kuwait - A desert country. Allergy 1997;52:1194-1200.
9. Dowaisan A, al-Ali S, Khan M, Hijazi Z, Thomson MS, Ezeamuzie CI: Sensitization to aeroallergens among patients with allergic rhinitis in a desert environment. Ann Allergy Asthma Immunol 2000;84:433-438.
10. Lombardero M, Duffort O, Sellés JG, Hernández J, Carreira J: Cross-reactivity among Chenopodiaceae and Amaranthaceae. Ann Allergy 1985;54:430-436.
11. Wurtzen PA, Nelson HS, Lowenstein H, Ipsen H: Characterization of Chenopodiales (Amaranthus retroflexus, Chenopodium album, Kochia scoparia, Salsola pestifer) pollen allergens. Allergy 1995;50:489-497.
12. Barderas R, Villalba M, Lombardero M, Rodríguez R: Identification and characterization of Che a 1 allergen from Chenopodium album pollen. Int Arch Allergy Immunol 2002;127:47-54.
13. Barderas R, Villalba M, Batanero E, Pascual CY, Rodríguez R: Role of profilin and polcalcin in chenopod pollen allergy. J Allergy Clin Immunol 2003;111:1132-1133.
14. Barderas R, Villalba M, Pascual CY, Batanero E, Rodríguez R: Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen. Isolation, amino acid sequences and immunological properties. J Allergy Clin Immunol 2004;113:1192-1198.
15. Villalba M, Batanero E, Monsalve RI, González de la Peña MA, Lahoz C, Rodríguez R: Cloning and expression of Ole e I, the major allergen from olive tree pollen. Polymorphism analysis and tissue specificity. J Biol Chem 1994;269:15217-15222.
16. Johnson MA, Preuss D: On your mark, get set, GROW! LePRK2-LAT52 interactions regulate pollen tube growth. Trends Plant Sci 2003;8:97-99.
17. Villalba M, Batanero E, López-Otín C, Sanchez LM, Monsalve RI, González de la Peña MA, Lahoz C, Rodríguez R: The amino acid sequence of Ole e I, the major allergen from olive tree (Olea europaea) pollen. Eur J Biochem 1993;216:863-869.
18. Calabozo B, Díaz-Perales A, Salcedo G, Barber D, Polo F: Cloning and expression of biologically active Plantago lanceolata pollen allergen Pla l 1 in the yeast Pichia pastoris. Biochem J 2003;372:889-896.
19. van Ree R, Hoffman DR, van Dijk W, Brodard V, Mahieu K, Koeleman CA, Grande M, van Leeuwen WA, Aalberse RC: Lol p XI, a new major grass pollen allergen, is a member of a family of soybean trypsin inhibitor-related proteins. J Allergy Clin Immunol 1995;95:970-978.
20. Calabozo B, Barber D, Polo F: Purification and characterization of the main allergen of Plantago lanceolata pollen, Pla l 1. Clin Exp Allergy 2001;31:322-330.
21. Huecas S, Villalba M, González E, Martínez-Ruiz A, Rodríguez R: Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris. Eur J Biochem 1999;261:539-546.
22. González E, Villalba M, Rodríguez R: Immunological and molecular characterization of the major allergens from lilac and privet pollens overproduced in Pichia pastoris. Clin Exp Allergy 2001;31:313-321.
23. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
24. Batanero E, Villalba M, Rodríguez R: Glycosylation site of the major allergen from olive tree pollen. Allergenic implications of the carbohydrate moiety. Mol Immunol 1994;31:31-37.
25. Batanero E, Crespo JF, Monsalve RI, Martín-Esteban M, Villalba M, Rodríguez R: IgE-binding and histamine-release capabilities of the main carbohydrate component isolated from the major allergen of olive tree pollen, Ole e 1. J Allergy Clin Immunol 1999;103:147-153.
26. 2+-binding proteins (polcalcins) involved in allergy. Eur J Biochem 1998;258:454-459.
27. Valenta R, Vrtala S, Laffer S, Spitzauer S, Kraft D: Recombinant allergens. Allergy 1998;53:552-561.
28. Trimble RB, Atkinson PH, Tschopp JF, Towsend RR, Maley F: Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris. J Biol Chem 1997;266:22807-22817.
29. 4-(N-acetyl- β-glucosaminyl) asparagines amidase F cannot release glycans with fucose attached α(1-3) to the asparagines-linked N-acetylglucosamine residue. Eur J Biochem 1991;199:647-652.
30. Batanero E, Villalba M, Monsalve RI, Rodríguez R: Cross-reactivity between the major allergen from olive pollen and unrelated glycoproteins: Evidence of an epitope in the glycan moiety of the allergen. J Allergy Clin Immunol 1996;97:1264-1271.
31. Calabozo B, Barber D, Polo F: Studies on the carbohydrate moiety of Pla l 1 allergen. Identification of a major N-glycan and significance for the immunoglobulin E-binding activity. Clin Exp Allergy 2002;32:1628-1634.