Structural Basis of Transcription: Separation of RNA from DNA by RNA Polymerase II

Science

Volumn 303, Issue 5660, 2004, Pages 1014-1016

  Westover, Kenneth D ^a   Bushnell, David A ^a   Kornberg, Roger D ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PROTEINS; SEPARATION;

HYBRID HELIX; POSTTRANSLOCATION STATE;

RNA;

DNA; RNA; RNA POLYMERASE II;

DNA; RNA;

ARTICLE; COMPLEX FORMATION; DNA RNA HYBRIDIZATION; DNA TEMPLATE; ENZYME STRUCTURE; PRIORITY JOURNAL; PROMOTER REGION; RNA STRUCTURE; STRUCTURE ANALYSIS; TRANSCRIPTION REGULATION;

BASE PAIRING; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA, SINGLE-STRANDED; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; NUCLEIC ACID HYBRIDIZATION; OLIGODEOXYRIBONUCLEOTIDES; OLIGORIBONUCLEOTIDES; PROMOTER REGIONS (GENETICS); PROTEIN CONFORMATION; RNA POLYMERASE II; RNA, COMPLEMENTARY; SACCHAROMYCES CEREVISIAE; TEMPLATES, GENETIC; TRANSCRIPTION FACTOR TFIIB; TRANSCRIPTION, GENETIC;

EID: 1142310578     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1090839     Document Type: Article

References (14)

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14. We thank N. Thompson and R. Burgess for providing antibody for protein purification, and Hewlett-Packard for providing a Unix workstation. This research is based in part on work done at Stanford Synchrotron Radiation Laboratory, which is funded by the U.S. Department of Energy (DOE) Office of Basic Energy Sciences. The structural biology program is supported by the NIH National Center for Research Resources Biomedical Technology Program and the DOE Office of Biological and Environmental Research. We thank the staff of the Berkeley Center for Structural Biology for help at the Advanced Light Source. D.A.B. was supported by postdoctoral fellowship PF-00-014-01-GMC from the American Cancer Society. K.D.W. was supported by the Medical Scientist Training Program. This research was supported by NIH grant GM49985 to R.D.K. Structure factors and coordinates have been deposited at the Protein Data Bank (PDB 1R9R).