Antisense oligonucleotides bound in the polysaccharide complex and the enhanced antisense effect due to the low hydrolysis

Biomaterials

Volumn 25, Issue 15, 2004, Pages 3117-3123

  Mizu, Masami ^a   Koumoto, Kazuya ^a   Anada, Takahisa ^a   Sakurai, Kazuo ^a   Shinkai, Seiji ^b  

^a UNIVERSITY OF KITAKYUSHU   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Antisense; Complexation; DNA; Gene expression; Polysaccharide

Indexed keywords

ASSAYS; DNA; HYDROLYSIS; POLYSACCHARIDES; PROTEINS;

ANTISENSE EFFECTS; DNA CODING;

BIOMATERIALS;

ANTISENSE OLIGONUCLEOTIDE; COMPLEMENTARY DNA; DEOXYRIBONUCLEASE; DRUG CARRIER; EXONUCLEASE; GREEN FLUORESCENT PROTEIN; NAKED DNA; PLASMID DNA; POLYSACCHARIDE;

ARTICLE; BINDING AFFINITY; BIOASSAY; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC CODE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL;

EID: 1142285428     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2003.11.008     Document Type: Article

References (21)

1. Tabata K., Ito W., Kojima T. Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune fries. Carbohydr Res. 89:1981;121-135.
2. Norisuye T., Yanaki T., Fujita H. Triple helix of a Schiphyllum commune polysaccharide in aqueous solution. J Polym Sci Polym Phys Ed. 18:1980;547-558.
3. Yanaki T., Norisuye T., Fujita H. Triple helix of Schizophyllum commune polysaccharide in dilute solution. 3. hydrodynamic properties in water. Macromolecules. 13:1980;1462-1466.
4. Sato T., Sakurai K., Norisuye T., Fujita H. Triple helix of Schizophyllum commune polysaccharide in dilute solution. Part VI. Collapse of randomly coiled schizophyllan in mixtures of water and dimethylsulfoxide. Polymer J. 15:1983;87-96.
5. McIntire T.M., Brant D.A. Observation of the (1→3)-b-D-glucan liner triple helix to macrocycle interconversion using noncontatct atomic force microscopy. J Am Chem Soc. 120:1998;6909-6919.
6. Sakurai K., Shinkai S. Molecular recognition of adenine, cytosine, and uracil in a single-stranded RNA by a natural polysaccharide. schizophyllan J Am Chem Soc. 122(18):2000;4520-4521.
7. Sakurai K., Mizu M., Shinkai S. Polysaccharide-polynucleotide complexes. 2. Complementary polynucleotide mimic behavior of the natural polysaccharide schizophyllan in the macromolecular complex with single-stranded RNA and DNA. Biomacromolecules. 2(3):2001;641-650.
8. Mizu M, Koumoto K, Kimura T, Sakurai K, Shinkai S. Protection of polynucleotides from nuclease-mediated hydrolysis by forming a complex with schizophyllan. The preceding submission.
9. Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
10. Technical data in Takara manual of GFP, BFP vectors, 2000 (Japanese).
11. AFPs™ AutoFlouresence Proteins, AFP-Manual (version AFP11No98), 2001.
12. Promega, Part# TB219. E. coli T7 S30 extract system for circular DNA, 2002.
13. Murata M., Kaku W., Anada T., Soh N., Katayama Y., Maeda M. Thermo responsive DNA/polymer conjugate for intelligent antisense strategy. Chem Lett. 32:2003;266-267.
14. Anada T., Kano T., Kaku W., Katayama Y., Maeda M. Functional regulation of biomolecule using DNA conjugate. Nucleic Acid Res Suppl. 2:2002;269-270.
15. Novagen EcoPro™ System TB278 06.
16. 2000 Novagen Catalog. p. 180, http://www.novagen com .
17. Mizu M, Kimura T, Koumoto K, Sakurai K, Shinkai S. Thermally induced conformational-transisiton of polydeoxyadenosine in the complex with schizophyllan and the base-length dependence of its stability. Chem Commun 2001;429-30.
18. Atale for four systems, optimized gene expression with the T7 sample system. Promega Note. Number 80, 2002.
19. Boussif O., Lezoualc'h F., Zanta M.A., Mergny M.D., Scherman D., Demeneix B., Behr J.P. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo. polyethylenimine Proc Natl Acad Sci USA. 92:1995;7297-7301.
20. Chirila T.V., Rakoczy P.E., Garrett K.L., Lou X., Constable I.J. The use of synthetic polymers for delivery of therapeutic antisense oligodeoxynucleotides. Biomaterials. 23(2):2002;321-342.
21. Koumoto K, Mizu M, Sakurai K, Kunitake T, Shinkai S. Intelligent release of the single-stranded DNA bound in the schizophyllan complex. Chem Biodiversity, submitted.