Quantification of parvovirus B19 DNA using COBAS ampliPrep automated sample preparation and LightCycler real-time PCR

Journal of Molecular Diagnostics

Volumn 6, Issue 1, 2004, Pages 37-41

  Schorling, Stefan ^a   Schalasta, Gunnar ^b   Enders, Gisela ^b   Zauke, Michael ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b Medical Diagnostic Laboratory   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN M; NUCLEIC ACID; VIRUS DNA;

ARTICLE; AUTOMATION; CONTROLLED STUDY; DEVICE; DIAGNOSTIC ACCURACY; DISEASE TRANSMISSION; DNA DETERMINATION; ERYTHEMA INFECTIOSUM; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOLATION PROCEDURE; NUCLEIC ACID ANALYSIS; PARVOVIRUS; PREGNANCY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; RISK ASSESSMENT; SENSITIVITY AND SPECIFICITY; SEROLOGY; TISSUE PREPARATION;

PARVOVIRUS;

EID: 1142263120     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1525-1578(10)60489-8     Document Type: Article

References (23)

1. Jungkind D: Automation of laboratory testing for infectious diseases using the polymerase chain reaction: our past, our present, our future. J Clin Virol 2001, 20:1-6
2. Stelzl E, Kormann-Klement A, Haas J, Daghofer E, Santner BI, Marth E, Kessler HH: Evaluation of an automated sample preparation protocol for quantitative detection of hepatitis C virus RNA. J Clin Microbiol 2002, 40:1447-1450
3. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J: Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990, 28:495-503
4. Anderson MJ, Jones SE, Fischer-Hoch SP, Lewis E, Hall SM, Bartlett CLR: Human parvovirus, the cause of erythema infectiosum (fifth disease)? Lancet 1983, 1:1378
5. Pattison JR, Jones SE, Hodgson J, Davis LR, White JM, Stroud CE, Murtaza L: Parvovirus infections and hypoplastic crisis in sickle-cell anaemia. Lancet 1989, 1:664-665
6. Cohen B: Parvovirus B19: an expanding spectrum of disease. Br Med J 1995, 311:1649-1552
7. Yoto Y, Kudoh T, Haseyama K, Suzuki N, Chiba S: Human parvovirus B19 infection associated with acute hepatitis. Lancet 1996, 347:868-901
8. Enders G, Dötsch J, Bauer J, Nützenadel W, Hengel H, Daffner D, Schalasta G, Searle K, Brown KE: Life-threatening parvovirus B19-associated myocarditis and cardiac transplantation as possible therapy: two case reports. Clin Infect Dis 1998, 26:355-358
9. Dux S, Lentini S, Bock CT, Klingel K, Kandolf R, Bauriedel G: Parvovirus B19 myocarditis in a young man with previous non-bacterial meningitis. Dtsch Med Wochenschr 2002, 127:1584-1588
10. Enders E: Infections of the fetus and the neonate other than rubella. Topley & Wilson's Microbiology and Microbial Infections. Edited by Collier L. London, Edward Arnold, 1998, pp 873-915
11. Brown KE: Haematological consequences of parvovirus B19 infection. Baillieres Best Pract Res Clin Haematol 2000, 13:245-259
12. Searle K, Schalasta G, Enders G: Development of antibodies to the nonstructural protein NS1 of parvovirus B19 during acute symptomatic and subclinical infection in pregnancy: implications for pathogenesis doubtful. J Med Virol 1998, 56:192-198
13. Searle K, Guilliard C, Wallat S, Schalasta G, Enders G: Acute parvovirus B19 infection in pregnant women: an analysis of serial samples by serological and semi-quantitative PCR techniques. Infection 1998, 26:139-143
14. Finney DJ: Probit Analysis, ed 3. Cambridge, Cambridge University Press, 1971
15. Neter J, Wassermann W, Kutner MH: Applied Linear Statistical Models: Regression, Analysis of Variance, and Experimental Designs. Homewood, IL, Irwin, 1985
16. Allain JP, Thomas I, Sauleda S: Nucleic acid testing for emerging viral infections. Transfus Med 2002, 12:275-283
17. Knoell A, Louwen F, Kochanowski B, Plentz A, Stussel J, Beckenlehner K, Jilg W, Modrow S: Parvovirus B19 infection in pregnancy: quantitative viral DNA analysis using a kinetic fluorescence detection system (TaqMan PCR). J Med Virol 2002, 67:259-266
18. Heegaard ED, Brown KE: Human parvovirus B19. Clin Microbiol Rev 2002, 15:485-505
19. Harder TC, Hufnagel M, Zahn K, Beutel K, Schmitt HJ, Ullmann U, Rautenberg P: New LightCycler PCR for rapid and sensitive quantification of parvovirus B19 DNA guides therapeutic decision-making in relapsing infections. J Clin Microbiol 2001, 39:4413-4419
20. Fini F, Gallinella G, Girotti S, Zerbini M, Musiani M: Development of a chemiluminescence competitive PCR for the detection and quantification of parvovirus B19 DNA using a microplate luminometer. Clin Chem 1999, 45:1391-1396
21. Fairley CK, Smoleniec JS, Caul OE, Miller E: Observational study of effect of intrauterine transfusions on outcome of fetal hydrops after parvovirus B19 infection. Lancet 1995, 346:1335-1337
22. Cassinotti P, Siegl G: Quantitative evidence for persistence of human parvovirus B19 DNA in an immunocompetent individual. Eur J Clin Microbiol Infect Dis 2000, 19:886-887
23. Musiani M, Zerbini M, Gentilomi G, Plazzi M, Gallinella G, Venturoli S: Parvovirus B19 clearance from peripheral blood after acute infection. J Infect Dis 1995, 172:1360-1363