Cloning and characterization of a novel transcript variant of IQGAP2 in human testis

DNA Sequence - Journal of DNA Sequencing and Mapping

Volumn 15, Issue 5-6, 2004, Pages 319-325

  Wang, Hui ^a   Huo, Ran ^a   Xu, Min ^a   Lu, Li ^a   Xu, Zhiyang ^a   Li, Jianmin ^a   Zhou, Zuomin ^a   Sha, Jiahao ^a  

^a NANJING MEDICAL UNIVERSITY   (China)

Author keywords

Alternative transcript; Hybridization; IQGAP2; Spermatogenesis

Indexed keywords

CALPONIN; COMPLEMENTARY DNA; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN;

ADULT; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHROMOSOME 5Q; DNA LIBRARY; DNA MICROARRAY; FETUS; GENE; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETIC VARIABILITY; HUMAN; HUMAN TISSUE; IQGAP2 GENE; MALE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN MOTIF; REGULATORY MECHANISM; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPERMATOGENESIS; TESTIS; TISSUE DISTRIBUTION; TISSUE SPECIFICITY;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; BASE SEQUENCE; DNA PRIMERS; GENE EXPRESSION; GENE LIBRARY; HUMANS; MALE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROTEIN STRUCTURE, TERTIARY; RAS GTPASE-ACTIVATING PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TESTIS;

EID: 11144281842     PISSN: 10425179     EISSN: None     Source Type: Journal    
DOI: 10.1080/10425170400009012     Document Type: Article

References (31)

1. Bahler, M. and Rhoads, A. (2002) "Calmodulin signaling via the IQ motif", FEBS Lett. 513(1), 107-113.
2. Beaudoing, E., Freier, S., Wyatt, J.R., Claverie, J.M. and Gautheret, D. (2000) "Patterns of variant polyadenylation signal usage in human genes", Genome Res. 10(7), 1001-1010.
3. Boguski, M.S. and McCormick, F. (1993) "Proteins regulating Ras and its relatives", Nature 366(6456), 643-654.
4. Bork, P. and Sudol, M. (1994) "The WW domain: a signalling site in dystrophin?", Trends Biochem. Sci. 19(12), 531-533.
5. Bourne, H.R., Sanders, D.A. and McCormick, F. (1990) "The GTPase superfamily: a conserved switch for diverse cell functions", Nature 348(6297), 125-132.
6. Briggs, M.W. and Sacks, D.B. (2003) "IQGAP proteins are integral components of cytoskeletal regulation", EMBO Rep. 4(6), 571-574.
7. Brill, S., Li, S., Lyman, C.W., Church, D.M., Wasmuth, J.J., Weissbach, L., Bernards, A. and Snijders, A.J. (1996) "The Ras GTPase-activating- protein-related human protein IQGAP2 harbors a potential actin binding domain and interacts with calmodulin and Rho family GTPases", Mol. Cell. Biol. 16(9), 4869-4878.
8. Chen, H.I. and Sudol, M. (1995) "The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules", Proc. Natl Acad. Sci. USA 92(17), 7819-7823.
9. Cheng, L.J., Zhou, Z.M., Li, J.M., Zhu, H., Zhu, H., Zhou, Y.D., Wang, L.R., Lin, M. and Sha, J.H. (2002) "Expression of a novel HsMCAK mRNA splice variant, tsMCAK gene, in human testis", Life Sci. 71(23), 2741-2757.
10. Cheng, L.J., Li, J.M., Chen, J., Ge, Y.H., Yu, Z.R., Han, D.S., Zhou, Z.M. and Sha, J.H. (2003) "NYD-SP16, a novel gene associated with spermatogenesis of human testis", Biol. Reprod. 68(1), 190-198.
11. Cooke, H.J., Hargreave, T. and Elliott, D.J. (1998) "Understanding the genes involved in spermatogenesis: a progress report", Fertil. Steril. 69(6), 989-995.
12. Eddy, E.M. (2002) "Male germ cell gene expression", Recent Prog. Horm. Res. 57, 103-128.
13. Escalier, D. (2001) "Impact of genetic engineering on the understanding of spermatogenesis", Hum. Reprod. Update 7(2), 191-210.
14. Kadonaga, J.T. (2002) "The DPE, a core promoter element for transcription by RNA polymerase II", Exp. Mol. Med. Sep. 30; 34(4), 259-264.
15. Letunic, I., Copley, R.R., Schmidt, S., Ciccarelli, F.D., Doerks, T., Schultz, J., Ponting, C.P. and Bork, P. (2004) "SMART 4.0: towards genomic data integration", Nucleic Acids Res. 1, 32.
16. MacDonald, C.C. and Redondo, J.L. (2002) "Reexamining the polyadenylation signal: were we wrong about AAUAAA?", Mol. Cell. Endocrinol. 190(1-2), 1-8.
17. Macias, M.J., Hyvonen, M., Baraldi, E., Schultz, J., Sudol, M., Saraste, M. and Oschkinat, H. (1996) "Structure of the WW domain of a kinase-associated protein complexed with a proline-rich peptide", Nature 382(6592), 646-649.
18. Maniatis, T. and Tasic, B. (2002) "Alternative pre-mRNA splicing and proteome expansion in metazoans", Nature 418(6894), 236-243.
19. McCallum, S.J., Erickson, J.W. and Cerione, R.A. (1998) "Characterization of the association of the actin-binding protein, IQGAP, and activated Cdc42 with Golgi membranes", J. Biol. Chem. 273(35), 22537-22544.
20. Means, A.R. (2000) "Regulatory cascades involving calmodulin-dependent protein kinases", Mol. Endocrinol. 14, 4-13.
21. Sassone-Corsi, P. (2002) "Unique chromatin remodeling and transcriptional regulation in spermatogenesis", Science 296(5576), 2176-2178.
22. Sha, J., Thou, Z., Li, L., Yin, L., Yang, H., Hu, G., Luo, M., Chan, H.C. and Zhou, K. and Spermatogenesis study group (2002) "Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays", Mol. Hum. Reprod. 8(6), 511-517.
23. Sudol, M., Sliwa, K. and Russo, T. (2001) "Functions of WW domains in the nucleus", FEBS Lett. 490(3), 190-195.
24. Weissbach, L., Settleman, J., Kalady, M.F., Snijders, A.J., Murthy, A.E., Yan, Y.X. and Bernards, A. (1994) "Identification of a human rasGAP-related protein containing calmodulin-binding motifs", J. Biol. Chem. 269(32), 20517-20521.
25. Wen, Y., Richardson, R.T. and O'rand, M.G. (1999) "Processing of the sperm protein Sp17 during the acrosome reaction and characterization as a calmodulin binding protein", Dev. Biol. 206(2), 113-122.
26. Wu, J.Y., Ribar, T.J. and Means, A.R. (2001) "Spermatogenesis and the regulation of Ca(2+)-calmodulin-dependent protein kinase IV localization are not dependent on calspermin", Mol. Cell. Biol. 21(17), 6066-6070.
27. Xiao, J., Xu, M., Li, J., Chang Chan, H., Lin, M., Zhu, H., Zhang, W., Zhou, Z., Zhao, B. and Sha, J. (2002) "NYD-SP6, a novel gene potentially involved in regulating testicular development/spermatogenesis", Biochem. Biophys. Res. Commun. 291(1), 101-110.
28. Xu, M., Xiao, J., Chen, J., Li, J., Yin, L., Zhu, H., Zhou, Z. and Sha, J. (2003) "Identification and characterization of a novel human testis-specific Golgi protein, NYD-SP12", Mol. Hum. Reprod. 9(1), 9-17.
29. Yin, L., Li, J., Zhu, H., Lin, M., Cheng, L., Wang, Y, Zhou, Z. and Sha, J. (2002) "Identification and characterization of a gene coding a novel isoform of DEAD-box protein", Reprod. Fertil. Dev. 14(3-4), 185-189.
30. Zhang, J. and Madden, T.L. (1997) "PowerBLAST: a new network BLAST application for interactive or automated sequence analysis and annotation", Genome Res. 7(6), 649-656.
31. Zhou, Z.M., Sha, J.H., Li, J.M., Lin, M., Zhu, H., Zhou, YD., Wang, L.R., Zhu, H., Wang, Y.Q. and Zhou, K.Y. (2002) "Expression of a novel reticulon-like gene in human testis", Reproduction 123(2), 227-234.