A high-throughput screening assay for kinases and phosphatases via metal ion-mediated fluorescent polymer superquenching

American Laboratory

Volumn 36, Issue 20, 2004, Pages 15-19

  Xia, Wensheng ^a   Achyuthan, Komandoor E ^a   Wittenburg, Shannon ^a   Rininsland, Frauke ^a  

^a QTL Biosystems   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BIOASSAY; BIOSENSORS; DRUG THERAPY; ENERGY TRANSFER; FLUORESCENCE; GALLIUM; PHOSPHATES; POSITIVE IONS; QUENCHING; RESONANCE;

FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET); LIGANDS; PHOSPHATE ASSAY; SUPERQUENCHING;

ENZYMES;

EID: 10944225994     PISSN: 00447749     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Review

References (5)

1. Cohen P. The origins of protein phosphorylation. Nat Cell Biol 2002; 4:E127-30.
2. Nick JA, Yong SK, Brown KK, et al. Role of p38 mitogen-activated protein kinase in a murine model of pulmonary in flammation. J Immunol 2000; 164:2151-9.
3. Chen L, McBranch DW, Wang HR, Helgeson R, Wudl F, Whitten DG. Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer. Proc Natl Acad Sci USA I999; 96:12,287-91.
4. + affinity chromawgraphy. Anal Biochem 1986; 154:250-4.
5. Xia W, Rininsland F, Wittenburg SK, et al. Applications of fluorescent polymer super quenching to high throughput screening assays for protein kinases. Assay Drug Devel Technol 2004; 2:183-92.