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-1 bands decreased substantially; this is associated with the tyrosines residues.[9] The conformational changes of RNase A were evaluated by using a method based on a combination of X-ray determined structures and Raman data.[10] After 23 Gy of irradiation, the α-helix percentage decreased from 18% to about 12% and the β-sheet percentage increased from about 48% to 54%. Increasing the dose to 61 Gy did not cause further conformational changes. It is worth noting that Met13, Met29, and Met30 are located in the α1 and α2 helices.
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0002660213
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10844249503
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Amino acid analysis and proteolytic mapping in conjunction with mass spectrometry is in progress.
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19
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10844291004
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note
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Three of the four methionines in RNase A are partially exposed on the surface.
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24
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10844289826
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-1, respectively;
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25
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10844261883
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note
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-1, respectively.
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33845557337
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