Heat Shock Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells

Cellular Physiology and Biochemistry

Volumn 14, Issue 1-2, 2004, Pages 41-46

  Kovář, Jan ^a   Štýbrová, Hana ^a   Novák, Petr ^b   Ehrlichová, Marie ^a   Truksa, Jaroslav ^a   Koc, Michal ^a   Kriegerbecková, Karin ^a   Scheiber Mojdehkar, Barbara ^c   Goldenberg, Hans ^c  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

^b INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

Heat shock protein 90; Hela cells; Iron binding protein; Plasma membrane

Indexed keywords

CHAPERONE; HEAT SHOCK PROTEIN 90; IRON BINDING PROTEIN;

ARTICLE; BINDING ASSAY; CELL MEMBRANE; CELL SURFACE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

EUKARYOTA;

EID: 10744231542     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000076925     Document Type: Article

References (32)

1. Csermely P, Schnaider T, Söti C, Prohászka Z, Nardai G: The 90-kDa molecular chaperone family: structure, function, and clinical applications. A comprehensive review. Pharmacol Ther 1998;79:129-168.
2. Scheibel T, Buchner J: The Hsp90 complex a super-chaperone machine as a novel drug target. Biochem Pharmacol 1998;56:675-682.
3. Young JC, Moarefi I, Hartl FU: Hsp90: a specialized but essential protein-folding tool. J Cell Biol 2001;154:267-273.
4. Garnier C, Lafitte D, Jorgensen TJD, Jensen ON, Briand C, Peyrot V: Phosphorylation and oligomerization states of native pig brain HSP90 studied by mass spectrometry. Eur J Biochem 2001;268:2402-2407.
5. Zhao YG, Gilmore R, Leone G, Coffey MC, Weber B, Lee PW: Hsp90 phosphorylation is linked to its chaperoning function. Assembly of the reovirus cell attachment protein. J Biol Chem 2001;276:32822-32827.
6. Ferrarini M, Heltai S, Zocchi MR, Rugarli C: Unusual expression and localization of heat-shock proteins in human tumor cells. Int J Cancer 1992;51:613-619.
7. Takata Y, Imamura T, Iwata M, Usui I, Haruta T, Nandachi N, Ishiki M, Sasaoka T, Kobayashi M: Functional importance of heat shock protein 90 associated with insulin receptor on insulin-stimulated mitogenesis. Biochem Biophys Res Commun 1997;237:345-347.
8. Stephanou A, Latchman DS, Isenberg DA: The regulation of heat shock proteins and their role in systemic lupus erythematosus. Semin Arthritis Rheum 1998;28:155-162.
9. Xu Y, Lindquist S: Heat-shock protein hsp90 governs the activity of pp60v-src kinase. Proc Natl Acad Sci USA 1993;90:7074-7078.
10. Aligue R, Akhavan-Niak H, Russell P: A role for Hsp90 in cell cycle control: Wee1 tyrosine kinase activity requires interaction with Hsp90. EMBO J 1994;13:6099-6106.
11. Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4. Genes Dev 1996;10:1491-1502.
12. van der Straten A, Rommel C, Dickson B, Hafen E: The heat shock protein 83 (Hsp83) is required for Raf-mediated signalling in Drosophila. EMBO J 1997;16:1961-1969.
13. Fukuda A, Osawa T, Oda H, Tanaka T, Toyokuni S, Uchida K: Oxidative stress response in iron-induced acute nephrotoxicity: enhanced expression of heat shock protein 90. Biochem Biophys Res Commun 1996;219:76-81.
14. Conconi M, Petropoulos I, Emod I, Turlin E, Biville F, Friguet B: Protection from oxidative inactivation of the 20S proteasome by heat-shock protein 90. Biochem J 1998;333:407-415.
15. Nardai G, Sass B, Eber J, Orosz G, Csermely P: Reactive cysteines of the 90-kDa heat shock protein, Hsp90. Arch Biochem Biophys 2000;384:59-67.
16. Hoffbrand A V, Ganeshaguru K, Hooton JWL, Tattersall MH: Effect of iron deficiency and desferrioxamine on DNA synthesis in human cells. Br J Haematol 1976;33:517-526.
17. Aisen P, Listowsky I: Iron transport and storage proteins. Annu Rev Biochem 1980;49:357-393.
18. van Eijk HG, de Jong G: The physiology of iron, transferrin, and ferritin. Biol Trace Elem Res 1992;35:13-24.
19. Brown JP, Woodbury RG, Hart CE, Hellström I, Hellström KE: Quantitative analysis of melanoma-associated antigen p97 in normal and neoplastic tissues. Proc Natl Acad Sci USA 1981;78:539-543.
20. Schlemmer SR, Sirotnak FM: Facile preparation of inside-out plasma membrane vesicles from tumor cells for functional studies of pharmacologically relevant translocating ATPases. Anal Biochem 1995;228:226-231.
21. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76-85.
22. Chen Y, Drysdale J: Detection of iron binding proteins by a blotting technique. Anal Biochem 1993;212:47-49.
23. Shevchenko A, Wilm M, Vorm O, Mann M: Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 1996;68:850-858.
24. Kriegerbeckova K, Kovar J: Role of melanotransferrin (p97) in nontransferrin iron uptake by HeLa and K562 cells. Folia Biol 2000;46:77-81.
25. Pollice AA, McCoy JP, Shackney SE, Smith CA, Agarwal J, Burholt DR, Janocko LE, Hornicek FJ, Singh SG, Hartsock RJ: Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins. Cytometry 1992;13:432-444.
26. Musilkova J, Kovar J: Additive stimulatory effect of extracellular calcium and potassium on non-transferrin ferric iron uptake by HeLa and K562 cells. Biochim Biophys Acta 2001;1514:117-126.
27. Furukawa T, Uchiumi T, Tokunaga R, Taketani S: Ribosomal protein P2, a novel iron-binding protein. Arch Biochem Biophys 1992;298:182-186.
28. cdk2 kinase. Blood 1995;86:2268-2280.
29. Kulp KS, Green SL, Vulliet PR: Iron deprivation inhibits cyclin-dependent kinase activity and decreases cyclin D/CDK4 protein levels in asynchronous MDA-MB-453 human breast cancer cells. Exp Cell Res 1996;229:60-68.
30. Kovar J, Stunz LL, Stewart BC, Kriegerbeckova K, Ashman RF, Kemp JD: Direct evidence that iron deprivation induces apoptosis in murine lymphoma 38C13. Pathobiology 1997;65:61-68.
31. Srethapakdi M, Liu F, Tavorath R, Rosen N: Inhibition of Hsp90 function by ansamycins causes retinoblastoma gene product-dependent G1 arrest. Cancer Res 2000;60:3940-3946.
32. Pandey P, Saleh A, Nakazawa A, Kumar S, Srinivasula SM, Kumar V, Weichselbaum R, Nalin C, Alnemri ES, Kufe D, Kharbanda S: Negative regulation of cytochrome c-mediated oligomerization of Apaf-1 and activation of procaspase-9 by heat shock protein 90. EMBO J 2000;19:4310-4322.