Cloning of Tetrahymena cells in a chemically defined medium is possible in the presence of surfactants or at reduced temperature

Journal of Biotechnology

Volumn 115, Issue 2, 2005, Pages 167-177

  Hellung Larsen, Per ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Cell death; Chemical stress; Cloning; Culturing; Physical stress; Pluronic; Surfactants; Survival

Indexed keywords

CHEMICALLY DEFINED MEDIUM (CDM); TETRAHYMENA CELLS;

BIOTECHNOLOGY; CELLS; HYDRODYNAMICS; SURFACE ACTIVE AGENTS;

CLONING;

BUFFER; GROWTH FACTOR; SURFACTANT;

ARTICLE; AUTOCRINE EFFECT; CELL DEATH; CELL GROWTH; CELL PROTECTION; CELL SUSPENSION; CONTROLLED STUDY; CULTURE MEDIUM; EXPOSURE; GROWTH REGULATION; HEAT STRESS; HYDRODYNAMICS; LOW TEMPERATURE; MOLECULAR CLONING; NONHUMAN; PHYSICAL STRESS; PRIORITY JOURNAL; STRESS; TEMPERATURE; TETRAHYMENA;

ANIMALS; APOPTOSIS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CLONING, MOLECULAR; CULTURE MEDIA; OXIDATIVE STRESS; POLOXAMER; SPECIES SPECIFICITY; SURFACE-ACTIVE AGENTS; TEMPERATURE; TETRAHYMENA;

TETRAHYMENA;

EID: 10644297220     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.08.006     Document Type: Article

References (28)

1. M.D. Amaral, L. Galego, and C. Rodrigues-Pousada Stress response of Tetrahymena pyriformis to arsenite and heat shock: differences and similarities Eur. J. Biochem. 171 1988 463 470
2. A.P. Andersen, and P. Hellung-Larsen Division competence in Tetrahymena: determination of minimum cell volume and rate of nutrient uptake J. Cell Biochem. 41 1989 125 133
3. BASF Corporation, 1999. Surfactants. Pluronic & Tetronic.
4. D. Cassidy-Hanley, H. Smith, and P. Bruns A simple, efficient technique for freezing Tetrahymena thermophila J. Euk. Microbiol. 42 1995 510 515
5. Y. Chisti Shear sensitivity 1999 John Wiley & Sons Inc.
6. S.T. Christensen, and L. Rasmussen Evidence for growth factors, which control cell multiplication in Tetrahymena thermophila Acta Protozool. 31 1992 215 219
7. S.T. Christensen, H. Sørensen, N. Beyer, K. Kristiansen, L. Rasmussen, and M.I. Rasmussen Cell death in Tetrahymena thermophila: new observations on culture conditions Cell Biol. Int. 25 2001 509 519
8. S.T. Christensen, D.N. Wheatley, M.I. Rasmussen, and L. Rasmussen Mechanisms controlling death, survival and proliferation in a model unicellular eukaryote Tetrahymena thermophila Cell Death Differ. 2 1995 301 308
9. Elliott, A.M., 1973. Biology of Tetrahymena. Dowden, Hutchinson and Ross, Stroudsburg, PL.
10. Frankel, J., 2000. Cell biology of Tetrahymena thermophila. Methods Cell Biol. 62, 27-125 (Acadmic Press).
11. S.D. Guttman, C.V.C. Glover, C.D. Allis, and M.A. Gorovsky Heat shock, deciliation and release from anoxia induce the synthesis of the same set of polypeptides in starved T. pyriformis Cell 22 1980 299 307
12. J.J. Hagemeister, M. Grave, T.B. Kristiansen, F. Assaad, and P. Hellung-Larsen Interface-mediated death of unconditioned Tetrahymena cells: effect of the medium composition J. Euk. Microbiol. 46 1999 6 11
13. P. Hellung-Larsen, and A.P. Andersen Cell volume and dry weight of Tetrahymena J. Cell Sci. 92 1989 319 324
14. P. Hellung-Larsen, and I. Lyhne Effect of shaking on the growth of diluted cultures of Tetrahymena J. Protozool. 39 1992 345 349
15. P. Hellung-Larsen, and I. Lyhne Certain fatty acids and steroids protect Tetrahymena from cell division stress caused by shaking Microbios 75 1993 241 247
16. P. Hellung-Larsen, F. Assaad, S. Pankratova, B.L. Saietz, and L. Skovgaard Effects of Pluronic F-68 on Tetrahymena cells: protection against chemical and physical stress and prolongation of survival under toxic conditions J. Biotechnol. 76 2000 185 195
17. P. Hellung-Larsen, I. Lyhne, A.P. Andersen, and U. Koppelhus Characteristics of dividing and non-dividing Tetrahymena cells at different physiological states Eur. J. Protistol. 29 1993 182 190
18. G.G. Holz, J. Erwin, N. Rosenbaum, and S. Aaronson Triparanol inhibition of Tetrahymena and its prevention by lipids Arch. Biochem. Biophys. 98 1962 312 322
19. T.B. Kristiansen, J.J. Hagemeister, M. Grave, and P. Hellung-Larsen Surface mediated death of unconditioned Tetrahymena cells: Effect of physical parameters, growth factors, hormones, and surfactants J. Cell Physiol. 169 1996 139 148
20. J.D. Michaels, J.E. Nowak, A.K. Mallik, K. Koczo, D.T. Wason, and E.T. Papoutsakis Analysis of cell-to-bubble attachment in sparged bioreactors in the presence of cell-protective additives Biotechnol. Bioeng. 47 1995 407 419
21. D.W. Murhammer, and C.F. Goochee Scale up of insect cultures: protective effects of pluronic F-68 Biotechnology 6 1988 1411 1418
22. L.A. Palomares, H. Gonzales, and O.T. Tamirez Evidence of Pluronic F-68 direct interaction with insect cells: impact on shear protection, recombinant protein, and baculovirus production Enzyme Microbiol. Technol. 26 2000 324 331
23. G. Parnaud, S. Tache, G. Peiffer, and D.E. Corpet Pluronic F 68 block polymer, a very potent suppressor of carcinogenesis in the colon of rats and mice Br. J. Cancer 84 2001 90 93
24. P.E. Ryals, and G.A. Thompson Alterations of the composition and size of the free fatty acid pool of Tetrahymena responding to low-temperature stress Biochim. Biophys. Acta 919 1987 122 131
25. T.W. Schulte, S. Akinaga, S. Soga, W. Sullivan, B. Stensgard, D. Toft, and L.M. Neckers Antibiotic radicicol binds to the N-terminal domain of Hsp90 and shares important biological activities with geldanamycin Cell Stress Chaperones 3 1998 100 108
26. C.E. Sripati An improved chemically defined medium for mass cultures of Tetrahymena: nutrient uptake and growth regulation J. Gen. Microbiol. 133 1987 2581 2588
27. L. Szablewski, P.H. Andreassen, A. Tiedtke, J. Florin-Christensen, M. Florin-Christensen, and L. Rasmussen Tetrahymena thermophila: growth in synthetic nutrient medium in the presence and absence of glucose J. Protozool. 38 1991 62 65
28. T. Togo, J.M. Alderton, G.-Q. Bi, and R.A. Steinhardt The mechanism of facilitated cell membrane resealing J. Cell Sci. 112 1999 719 731