A rapid method for the discrimination of genes encoding classical Shiga toxin (Stx) 1 and its variants, Stx1c and Stx1d, in Escherichia coli

Molecular Nutrition and Food Research

Volumn 48, Issue 7, 2004, Pages 515-521

  Kuczius, Thorsten ^a   Bielaszewska, Martina ^a   Friedrich, Alexander W ^a   Zhang, Wenlan ^a  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

Author keywords

Escherichia coli; Melting curve analysis; Real time fluorescence polymerase chain reaction; Shiga toxin

Indexed keywords

SHIGA TOXIN; SHIGA TOXIN 1C; SHIGA TOXIN 1D; UNCLASSIFIED DRUG; VEROTOXIN 1;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; DIARRHEA; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; FOOD CONTAMINATION; HEMOLYTIC UREMIC SYNDROME; HYBRIDIZATION; MELTING POINT; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; RISK ASSESSMENT; SEQUENCE ANALYSIS;

DNA, BACTERIAL; ESCHERICHIA COLI; FLUORESCENCE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SHIGA TOXIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 10444254044     PISSN: 16134125     EISSN: None     Source Type: Journal    
DOI: 10.1002/mnfr.200400038     Document Type: Article

References (32)

1. Gerber, A., Karch, H., Allerberger, F., Verweyen, H. M., Zimmerhackl, L., Clinical course and the role of Shiga toxin-producing Escherichia coli infection in the hemolytic-uremic syndrome in pediatric patients, 1997-2000, in Germany and Austria: a prospective study. J. Infect. Dis. 2002, 186, 493-500.
2. Werber, W., Fruth, A., Liesegang, A., Littmann, M., et al., A multistate outbreak of Shiga toxin-producing Escherichia coli O26:H11 infections in Germany, detected by molecular subtyping surveillance. J. Infect. Dis. 2002, 186, 419-422.
3. Allerberger, F., Friedrich, A. W., Grif, K., Dierich, M. P., et al., Hemolytic-uremic syndrome associated with enterohemorrhagic Escherichia coli O26:H infection and consumption of unpasteurized cow's milk. Int. J. Infect. Dis. 2003, 7, 42-45.
4. Misselwitz, J., Karch, H., Bielaszewska, M., John, U., et al., Cluster of hemolytic-uremic syndrome caused by Shiga toxin-producing Escherichia coli O26:H11. Pediatr. Infect. Dis. J. 2003, 22, 349-354.
5. Sonntag, A. K., Prager, R., Bielaszewska, M., Zhang, W., et al., Phenotypic and genotypic analyses of enterohemorrhagic Escherichia coli 0145 strains from patients in Germany. J. Clin. Microbiol. 2004, 42, 954-962.
6. Geue, L., Segura-Alvarez, M., Conraths, F. J., Kuczius, T., et al., A long-term study on the prevalence of Shiga toxin-producing Escherichia coli (STEC) on four German cattle farms. Epidemiol. Infect. 2002, 129, 173-185.
7. O'Brien, A. D., Tesh, V. L., Donohue-Rolfe, A., Jackson, M. P., et al., Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesis. Curr. Top. Microbiol. Immunol. 1992, 180, 65-94.
8. Schmitt, C. K., McKee, M. L., O'Brien, A. D., Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H-strain E32511. Infect. Immun. 1991, 59, 1065-1073.
9. Jelacic, J. K., Damrow, T., Chen, G. S., Jelacic, S., et al., Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles. J. Infect. Dis. 2003, 188, 719-729.
10. Melton-Celsa, A. R., Darnell, S. C., O'Brien, A. D., Activation of Shiga-like toxins by mouse and human intestinal mucus correlates with virulence of enterohemorrhagic Escherichia coli O91:H21 isolates in orally infected, streptomycin-treated mice. Infect. Immun. 1996, 64, 1569-1576.
11. Pierard, D., Muyldermans, G., Moriau, L., Stevens, D., Lauwers, S., Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates. J. Clin. Microbiol. 1998, 36, 3317-3322.
12. Weinstein, D. L., Jackson, M. P., Samuel, J. E., Holmes, R. K., O'Brien, A. D., Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine. J. Bacteriol. 1988, 170, 4223-4230.
13. Schmidt, H., Scheef, J., Morabito, S., Caprioli, A., et al., A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl. Environ. Microbiol. 2000, 66, 1205-1208.
14. Paton, A. W., Paton, J. C., Goldwater, P. N., Heuzenroeder, M. W., Manning, P. A., Sequence of a variant Shiga-like toxin type-I operon of Escherichia coli O111:H-. Gene 1993, 129, 87-92.
15. Paton, A. W., Beutin, L., Paton, J. C., Heterogeneity of the amino-acid sequences of Escherichia coli Shiga-like toxin type-I operons. Gene 1995, 153, 71-74.
16. 1c) in Escherichia coli strains isolated from humans. J. Clin. Microbiol. 2002, 40, 1441-1446.
17. Bürk, C., Dietrich, R., Acar, G., Moravek, M., et al., Identification and characterization of a new variant of Shiga toxin 1 in Escherichia coli ONT:H19 of bovine origin. J. Clin. Microbiol. 2003, 47, 2106-2112.
18. Friedrich, A. W., Borell, J., Bielaszewska, M., Fruth, A., et al., Shiga toxin 1c-producing Escherichia coli strains: phenotypic and genetic characterization and association with human disease. J. Clin. Microbiol. 2003, 41, 2448-2453.
19. Schmidt, H., Rüssmann, H., Schwarzkopf, A., Aleksic, S., et al., Prevalence of attaching and effacing Escherichia coli in stool samples from patients and controls. Zentralbl. Bakteriol. 1994, 257, 201-213.
20. Cebula, T. A., Payne, W. L., Feng, P., Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J. Clin. Microbiol. 1995, 33, 248-250.
21. Franke, S., Harmsen, D., Caprioli, A., Pierard, D., et al., Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 strains of human and porcine origin. J. Clin. Microbiol. 1995, 33, 3174-3178.
22. Friedrich, A.W., Bielaszewska, M., Zhang, W. L., Pulz, M., et al., Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J. Infect. Dis. 2002, 155, 74-84.
23. Prager, R., Strutz, U., Fruth, A., Tschäpe, H., Subtyping of pathogenic Escherichia coli strains using flagellar (H)-antigens: serotyping versus fliC polymorphisms. Int. J. Med. Microbiol. 2003, 292, 477-486.
24. Rüssmann, H., Schmidt, H., Heesemann, J., Caprioli, A., Karch, H., Variants of Shiga-like toxin II constitute a major toxin component in Escherichia coli O157 strains from patients with haemolytic uraemic syndrome. J. Med. Microbiol. 1994, 40, 338-343.
25. Reischl, U., Youssef, M. T., Kilwinski, J., Lehn, N., et al., Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli. J. Clin. Microbiol. 2002, 40, 2555-2565.
26. Livak, K. J., Flood, S. J., Marmaro, J., Giusti, W., Deetz, K., Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 1995, 4, 357-362.
27. Wittwer, C. T., Herrmann, M. G., Moss, A. A., Rasmussen, R. P., Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques 1997, 22, 134-138.
28. Wittwer, C. T., Ririe, K. M., Andrew, R. V., David, D. A., et al., The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. BioTechniques 1997, 22, 176-181.
29. Zhang, W., Kohler, B., Oswald, E., Beutin, L., et al., Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J. Clin. Microbiol. 2002, 40, 4486-4492
30. Bielaszewska, M., Fell, M., Greune, L., Prager, R., et al., Characterization of cytolethal distending toxin genes and expression in Shiga toxin-producing Escherichia coli strains of non-O157 serogroups. Infect. Immun. 2004, 72, 1812-1816.
31. Dobrindt, U., Agerer, F., Michaelis, K., Janka, A., et al., Analysis of genome plasticity in pathogenic and commensal Escherichia coli isolates by use of DNA arrays. J. Bacteriol. 2003, 185, 1831-1840.
32. Ludwig, K., Sarkim, V., Bitzan, M., Karmali, M. A., et al., Shiga toxin-producing Escherichia coli infection and antibodies against Stx2 and Stx1 in household contacts of children with enteropathic hemolytic-uremic syndrome. J. Clin. Microbiol. 2002, 40, 1773-1782.