Automated higher-throughput compound screening on ion channel targets based on the Xenopus laevis oocyte expression system

Assay and Drug Development Technologies

Volumn 2, Issue 5, 2004, Pages 515-524

  Pehl, Ulrich ^a   Leisgen, Christine ^b   Gampe, Kristine ^a   Guenther, Elke ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b MULTI CHANNEL SYSTEMS MCS GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID A RECEPTOR; BICUCULLINE; COMPLEMENTARY DNA; COMPLEMENTARY RNA; ION CHANNEL; POTASSIUM;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTOMATION; CONTROLLED STUDY; DRUG SCREENING; DRUG TARGETING; FEMALE; GENE EXPRESSION SYSTEM; HIGH THROUGHPUT SCREENING; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYTE; POTASSIUM CURRENT; QUALITY CONTROL; REPRODUCIBILITY; VOLTAGE CLAMP; XENOPUS LAEVIS;

ANIMALS; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DELIVERY SYSTEMS; FEMALE; GAMMA-AMINOBUTYRIC ACID; GENE EXPRESSION REGULATION; INJECTIONS; ION CHANNELS; OOCYTES; RECEPTORS, GABA-A; ROBOTICS; XENOPUS LAEVIS;

EID: 10244256419     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/1540658042584071     Document Type: Article

References (41)

1. Lehmann-Horn F, Jurkat-Rott K: Voltage-gated ion channels and hereditary disease. Physiol Rev 1999;79:1317-1372.
2. Willumsen NJ, Bech M, Olesen SP, Jensen BS, Korsgaard MPG, Christophersen P: High throughput electrophysiology: new perspectives for ion channel drug discovery. Receptors Channels 2003;9:3-12.
3. Scolnick E: The re-emergence of ion channel drug discovery. Windhover's Rev Emerg Med Ventures 2003;8(5):9.
4. Anderson RN, Smith BL: Deaths: leading causes for 2001. Natl Vital Stat Rep 2003;52(9):1-85.
5. Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, et al.: The sequence of the human genome. Science 2001;291:1304-1351.
6. Xu J, Wang X, Ensign B, Li M, Wu L, Guia A: Ion-channel assay technologies: quo vadis? Drug Discov Today 2001;6:1278-1287.
7. Owen D, Silverthorne A: Channelling drug discovery. Current trends in ion channel drug discovery research. Drug Discov World 2002;3(2):48-61.
8. Xu J, Wu L, Wang X, Guia A, Yang D, Huang M, Cui C, Xu J: Novel chip designs for high-throughput electrophysiology [abstract 786]. In: Biophysics Society Meeting. Biophysics Society, San Francisco, 2002.
9. Soreq H: The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes. Crit Rev Biochem 1985;18:199-238.
10. Miledi R, Parker I, Sumikawa K: Synthesis of chick brain GABA receptors by frog oocytes. Proc R Soc Lond B Biol Sci 1982;216:509-515.
11. Methfessel C, Witzemann V, Takahashi T, Mishina M, Numa S, Sakmann B: Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and 19 implanted acetylcholine receptor and sodium channels. Pflugers Arch 1986; 407:577-588.
12. Dascal N: The use of Xenopus oocytes for the study of ion channels. Crit Rev Biochem 1987;22:317-387.
13. Gurdon JB, Lane CD, Woodland HR, Marbaix G: Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature 1971;233:177-182.
14. Gurdon JB, Wickens MP: The use of Xenopus oocytes for the expression of cloned genes. Methods Enzymol 1983; 101:370-386.
15. Stühmer W, Parekh AB: Recording from Xenopus oocytes. In: Single Channel Recording, 2nd ed. (Sakmann B, and Neher E, eds.), pp. 341-356. Plenum, New York, 1995.
16. Stühmer W, Terlau H, Heinemann SH: Xenopus oocytes for two-electrode and patch clamp Recording. In: Practical Electrophysiological Methods (Kettenmann H, Grantyn R, eds.), pp. 121-125. Wiley-Liss, New York, 1992.
17. Snutch TP: The use of Xenopus oocytes to probe synaptic communication. Trends Neurosci 1988;11:250-256.
18. Miledi R, Parker I, Sumikawa K: Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes. EMBO J 1982;1:1307-1312.
19. Timpe LC, Schwarz TL, Tempel BL, Papazian DM, Jan YN, Jan LY: Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes. Nature 1988; 331:143-145.
20. Marshall J, Buckingham SD, Shingai R, Lunt GG, Goosey MW, Darlison MG, Sattelle DB, Barnard EA: Sequence and functional expression of a single alpha subunit of an insect nicotinic acetylcholine receptor. EMBO J 1990;9: 4391-4398.
21. Bertrand D, Ballivet M, Gomez M, Bertrand S, Phannavong B, Gundelfinger ED: Physiological properties of neuronal nicotinic receptors reconstituted from the vertebrate beta 2 subunit and Drosophila alpha subunits. Eur J Neurosci 1994;6:869-875.
22. Bertrand D, Buisson B, Krause RM, Hu HY, Bertrand S: Electrophysiology: a method to investigate the functional properties of ligand-gated channels. J Recept Signal Transduct Res 1997;17:227-242.
23. Schroeder JI: Heterologous expression of higher plant transport proteins and repression of endogenous ion currents in Xenopus oocytes. Methods Cell Biol 1995;50: 519-533.
24. A-benzodiazepine receptors. Trends Pharmacol Sci 1989;10:407-411.
25. A receptor channels: from subunits to functional entities. Curr Opin Neurobiol 1992;2:263-269.
26. Lolait O'Carroll AM, Kusano K, Mahan LC: Pharmacological characterization and region-specific expression in brain of the beta 2- and beta 3-subunits of the rat GABAA receptor. FEBS Lett 1989;258:17-21.
27. Wagner DA, Czajkowski C: Structure and dynamics of the GABA binding pocket: a narrowing cleft that constricts during activation. J Neurosci 2001;21:67-74.
28. Ks in guinea pig cardiac myocytes. Biochem Biophys Res Commun 1994;202:265-270.
29. Ks cardiac potassium current. Nature 1996; 384:78-80.
30. Ks potassium channel. Nature 1996;384:80-83.
31. minK channel complex. Trends Pharmacol Sci 1997; 18:26-29.
32. Schnizler K, Küster M, Methfessel C, Fejtl M: The Roboocyte: automated cDNA/mRNA injection and subsequent TEVC recording on Xenopus oocytes in 96-well microtiter plates. Receptors Channels 2003;9:41-48.
33. Dubin A, Defazio T, Rohrbacher J: Identifying hERG modulators with an automated patch clamp system. Soc Biomol Screen Conf 2003;9:1403.
34. sK channels by the chromanol 293B. Pflugers Arch 1996;432: 1094-1096.
35. sK channels in epithelia. FEBS Lett 1996; 396:271-275.
36. Ks by chromanols. Br J Pharmacol 2000;131:1503-1506.
37. Dumont JN: Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol 1972;136:153-179.
38. Connolly CN, Krishek BJ, McDonald BJ, Smart TG, Moss SJ: Assembly and cell surface expression of heteromeric and homomeric γ-aminobutyric acid type A receptors. J Biol Chem 1996; 271:89-96.
39. + channel KCNQ1 to cytosolic PH changes and alters its pharmacology and sensitivity to temperature. Pflugers Arch 2000; 441:368-378.
40. + current by chromanol 293B in guinea-pig ventricular cells. Br J Pharmacol 2000; 129:1007-1013.
41. Sun Z-Q, Thomas GP, Antzelevitch C: Chromanol 293B inhibits slowly activating delayed rectifier and transient outward currents in canine left ventricular myocytes. J Cardiovasc Electrophysiol 2001;12:472-478.