Ablation of the murine α myosin heavy chain gene leads to dosage effects and functional deficits in the heart

Journal of Clinical Investigation

Volumn 98, Issue 8, 1996, Pages 1906-1917

  Jones, W Keith ^a   Grupp, Ingrid L ^b   Doetschman, Thomas ^b   Grupp, Gunter ^c   Osinska, Hanna ^a   Hewett, Timothy E ^a   Boivin, Greg ^c   Gulick, James ^a   Ng, Willie A ^a   Robbins, Jeffrey ^a  

^a CINCINNATI CHILDREN S HOSPITAL RESEARCH FOUNDATION   (United States)

^b Dept Pharmacol and Cell Biophys ^*

^c UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

embryonic stem cells; gene dosage; gene targeting; left ventricular function; myofibrils; myosin

Indexed keywords

CONTRACTILE PROTEIN; MYOSIN HEAVY CHAIN;

ALLELE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; GENE DOSAGE; GENE MUTATION; GENE TARGETING; HEART DISEASE; HEART LEFT VENTRICLE FUNCTION; HEART MUSCLE CONTRACTILITY; HETEROZYGOTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SARCOMERE; STEM CELL;

EID: 10244221064     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI118992     Document Type: Article

References (29)

1. Lyons, G.E., S. Schiaffino, D. Sassoon, P. Barton, and M. Buckingham. 1990. Developmental regulation of myosin gene expression in mouse cardiac muscle. J. Cell Biol. 111:2427-2436.
2. Ng, W.A., I.L. Grupp, A. Subramaniam, and J. Robbins. 1991. Cardiac myosin heavy chain mRNA expression and myocardial function in the mouse heart. Circ. Res. 68:1742-1750.
3. Izumo, S., B. Nadal-Ginard, and V. Mahdavi. 1986. All members of the MHC multigene family respond to thyroid hormone in a highly tissue-specific manner. Science (Wash. DC). 231:597-600.
4. Seidman, C.E., and J.G. Seidman. 1991. Mutations in cardiac myosin heavy chain genes cause familial hypertrophic cardiomyopathy. Mol. Biol. Med. 8:159-166.
5. Lankford, E.B., N.D. Epstein, L. Fananapazir, and H.L. Sweeney. 1995. Abnormal contractile properties of muscle fibers expressing beta-myosin heavy chain gene mutations in patients with hypertrophic cardiomyopathy. J. Clin. Invest. 95:1409-1414.
6. Straceski, A., C.E. Geisterfer-Lowrance, C.E. Seidman, J.G. Seidman, and L. Leinwand. 1994. Functional analysis of myosin missense mutations in familial hypertrophic cardiomyopathy. Proc. Natl. Acad. Sci. USA. 91:589-593.
7. Geisterfer-Lowrance, A.A.T., M. Christe, D.A. Conner, J.S. Ingwall, F.J. Schoen, C.E. Seidman, and J.G. Seidman. 1996. A mouse model of familial hypertrophic cardiomyopathy. Science (Wash. DC). 272:731-734.
8. Beall, C.J., M.A. Sepanski, and E.A. Fyrberg. 1989. Genetic dissection of Drosophila myofibril formation: effects of actin and myosin heavy chain null alleles. Genes Dev. 3:131-140.
9. Bernstein, S.I., P.T. O'Donnell, and R.M. Cripps. 1993. Molecular genetic analysis of muscle development, structure, and function in Drosophila. Int. Rev. Cytol. 143:63-152.
10. Brown, D.G., V.N. Warren, P. Pahlsson, and S.J. Kimber. 1993. Carbohydrate antigen expression in murine embryonic stem cells and embryos, I. Histochem. J. 25:452-463.
11. Kimber, S.J., D.G. Brown, P. Pahlsson, B. Nilsson, D.G. Brown, V. N. Warren, P. Pahlsson, S.J. Kimber, S. Sawai, A. Shimono, et al. 1993. Carbohydrate antigen expression in murine embryonic stem cells and embryos, II. Histochem. J. 25:628-641.
12. Gossler, A., T.C. Doetschman, R. Korn, E. Serfling, and R. Kemler. 1986. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc. Natl. Acad. Sci. USA. 83:9065-9069.
13. Chan, S.Y., and M.J. Evans. 1991. In situ freezing of embryonic stem cells in multiwell plates. Trends Genet. 7:76.
14. Robbins, J., J. Gulick, A. Sánchez, P. Howles, and T. Doetschman. 1990. Mouse embryonic stem cells express the cardiac myosin heavy chain genes during development in vitro. J. Biol. Chem. 265:11905-11909.
15. Sánchez, A., W.K. Jones, J. Gulick, T.C. Doetschman, and J. Robbins. 1991. Myosin heavy chain gene expression in mouse embryoid bodies. J. Biol. Chem. 266:22419-22426.
16. Subramaniam, A., W.K. Jones, J. Gulick, S. Wert, J. Neumann, and J. Robbins. 1991. Tissue-specific regulation of the α-myosin heavy chain gene promoter in transgenic mice. J. Biol. Chem. 266:24613-24620.
17. Rudnicki, M.A., G. Jackowski, L. Saggin, and M.W. McBurney. 1990. Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells. Dev. Biol. 138:348-358.
18. Grupp, I.L., A. Subramaniam, T.E. Hewett, J. Robbins, and G. Grupp. 1993. Comparison of normal, hypodynamic, and hyperdynamic mouse hearts using isolated work-performing heart preparations. Am. J. Physiol. 265:H1401-1411.
19. Hewett, T.E., I.L. Grupp, G. Grupp, and J. Robbins. 1994. Alpha-skeletal actin is associated with increased contractility in the mouse heart. Circ. Res. 74:740-746.
20. Daniel, Wayne W. 1987. Biostatistics: A Foundation for Analysis in the Health Sciences. John Wiley and Sons, Inc. Third ed. 1983. 276-289. New York, NY.
21. Bowerman, B.L., R.T. O'Connell, and D.A. Dickey. 1986. Linear Statistical Models: an Applied Approach. PWS/Duxbury Press, Boston, MA. 343-351.
22. Schwinger, R.H., M. Bohm, A. Koch, U. Schmidt, I. Morano, H.J. Eissner, P. Uberfuhr, B. Reichart, and E. Erdmann. 1994. The failing human heart is unable to use the Frank-Starling mechanism. Circ. Res. 74:959-969.
23. Sun, Y., and K.T. Weber. 1996. Angiotensin-converting enzyme and wound healing in diverse tissues of the rat. J. Lab. Clin. Med. 127:94-101.
24. Chun, M., and S. Falkenthal. 1988. Ifm(2)2 is a myosin heavy chain allele that disrupts myofibrillar assembly only in the indirect flight muscle of Drosophila melanogaster. J. Cell. Biol. 107:2613-2621.
25. Boluy, M.O., L.O. Neill, A.L. Meredith, O.H.L. Bing, W.W. Brooks, C.H. Conrad, M.T. Crow, and E.G. Lakatta. 1994. Alterations in cardiac gene expression during the transition from stable hypertrophy to heart failure. Circ. Res. 75:23-32.
26. Schwartz, K., L. Carrier, C. Chassagne, C. Wisnewsky, and K.R. Boheler. 1992. Regulation of myosin heavy chain and actin isogenes during cardiac growth and hypertrophy. Symp. Soc. Exp. Biol. 46:265-272.
27. Schwartz, K., C. Chassagne, and K.R. Boheler. 1993. The molecular biology of heart failure. J. Am. Coll. Cardiol. 22:(4 Supp. A), 30A.
28. Hein, S., D. Scholz, N. Fujitani, H. Rennollet, T. Brand, A. Friedl, and J. Schaper. 1994. Altered expression of titin and contractile proteins in failing human myocardium. J. Mol. Cell Card. 26:1291-1306.
29. Morano, I., K. Hadicke, S. Grom, A. Kock, R.H. Schwinger, M. Bohm, S. Bartel, E. Erdmann, and E.G. Krause. 1994. Titin, myosin light chains and C-protein in the developing and failing human heart. J. Mol. Cell Cardiol. 26: 361-368.