A mouse model of familial hypertrophic cardiomyopathy

Science

Volumn 272, Issue 5262, 1996, Pages 731-734

  Geisterfer Lowrance, Anja A T ^b   Christe, Michael ^b   Conner, David A ^b   Ingwall, Joanne S ^b   Schoen, Frederick J ^b   Seidman, Christine E ^b   Seidman, J G ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

MYOSIN HEAVY CHAIN;

AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEMALE; GENE MUTATION; HEART DISEASE; HEART HYPERTROPHY; HEART MUSCLE FIBROSIS; HISTOPATHOLOGY; HYPERTROPHIC CARDIOMYOPATHY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SEX DIFFERENCE;

ANIMALIA;

EID: 0030067394     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5262.731     Document Type: Article

References (29)

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18. The subaortic interventricular septum and left ventricular free wall were 2.5 and 3 times as thick, respectively, as the apical wall (0 9 to 1.1 mm).
19. Exons 13 and 14 are fused into a single exon (designated exon 13 in Fig. 1) in mouse α and β cardiac MHC genes (A A. T. Geisterfer-Lowrance, C. E Seidman, J. G. Seidman, unpublished data).
20. J. H. Weis and T Quertermous, in Current Protocols in Molecular Biology, F. M Ausubel et al , Eds. (Wiley, New York, 1994), chap 5.
21. The primers used for introducing the R403Q mutation were 5′-GCCTGTGTCACCCTCAGGTGAAG-GTGGGGAACGAGTATGTC-3′ and 5′-TTCCCCA-CCTTCACCTGAGGGTGACACAGGCCCTTGAGC-AG-3′
22. B A. Hendrickson et el , J. Exp Med 182 1905 (1995); W Zhang et al., ibid. 183, 1 (1996) The construct was linearized with Eco RV (located in intron 8 of the α cardiac MHC gene), introduced into C1 ES cells by electraporation, and grown in selective media.
23. B A. Hendrickson et el , J. Exp Med 182 1905 (1995); W Zhang et al., ibid. 183, 1 (1996) The construct was linearized with Eco RV (located in intron 8 of the α cardiac MHC gene), introduced into C1 ES cells by electraporation, and grown in selective media.
24. The targeting construct and the endogenous α cardiac MHC gene underwent homologous recombination in about I of 50 neomycin-resistant cells (A A T Geisterfer-Lowrance, M. Christe, D. A. Conner, J. S Ingwall, F. J. Schoen, C. E. Seidman, J. G. Seidman, data not shown).
25. 6 targeted ES cells grew in fialuridine (FIAU)-containing media The desired intrachromosomal recombination event occurred more frequently in cells that were grown for only a few hours without G418 before selection in HAD (A A. T. Geisterfer-Lowrance, M Christe, D. A. Conner, J. S. Ingwall, F J. Schoen, C. E Seidman, J. G. Seidman, data not shown).
26. Because the pUC18 sequences in the targeting construct contained three Dra I sites, the Dra I fragment encoding the endogenous exon 13/14 of the α cardiac MHC gene was shorter after the insertion of the targeting construct.
27. Exon 13/14 was amplified with primers 5′-GGACA-AAGGAATGGAGGTACTGAAA-3′ and 5′-CTGATG-GTCTGAGTGGGTAGGTGAG-3′.
28. -1 (calibrated at constant flow). Positive and negative dP/dt were determined on-line.
29. The care of all animals used in these studies was in accordance with institutional and IACUC guidelines. Supported in part by grants from the Howard Hughes Medical Institute and NIH.