Soybean galactinol synthase forms fagopyritol B1 but not galactopinitols: Substrate feeding of isolated embryos and heterologous expression

Seed Science Research

Volumn 14, Issue 4, 2004, Pages 321-333

  Obendorf, Ralph L ^a   Odorcic, Silvia ^a   Ueda, Takashi ^a,b   Coseo, Mark P ^a   Vassallo, Elizabeth ^a  

^a Cornell University ^*  (United States)

^b FLORIDA GULF COAST UNIVERSITY   (United States)

Author keywords

Cyclitol substrate; D chiro inositol; Fagopyritol synthesis; Galactinol synthase; Glycine max; Heterologous expression; Soybean embryo

Indexed keywords

ESCHERICHIA COLI; GLYCINE MAX;

EID: 10044241389     PISSN: 09602585     EISSN: None     Source Type: Journal    
DOI: 10.1079/SSR2004186     Document Type: Article

References (35)

1. Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 100, 225-230.
2. Carmi, N., Zhang, G.F., Petreikov, M., Gao, Z.F., Eyal, Y., Granot, D. and Schaffer, A.A. (2003) Cloning and functional expression of alkaline α-galactosidase from melon fruit: similarity to plant SIP proteins uncovers a novel family of plant glycosyl hydrolases. Plant Journal 33, 97-106.
3. Chanprame, S., Kuo, T.M. and Widholm, J.M. (1998) Soluble carbohydrate content of soybean [Glycine max (L.) Merr.] somatic and zygotic embryos during development. In Vitro Cellular and Developmental Biology-Plant 34, 64-68.
4. Dittrich, P. and Brandl, A. (1987) Revision of the pathway of D-pinitol formation in Leguminosae. Phytochemistry 26, 1925-1926.
5. Frydman, R.B. and Neufeld, E.F. (1963) Synthesis of galactosylinositol by extracts from peas. Biochemical and Biophysical Research Communications 12, 121-125.
6. Handley, L.W., Pharr, D.M. and McFeeters, R.E (1983) Relationship between galactinol synthase activity and sugar composition of leaves and seeds of several crop species. Journal of the American Society for Horticultural Science 108, 600-605.
7. Hegeman, C.E., Good, L.L. and Grabau, E.A. (2001) Expression of D-myo-inositol-3-phosphate synthase in soybean. Implications for phytic acid biosynthesis. Plant Physiology 125, 1941-1948.
8. Hitz, W.D., Carlson, T.J., Kerr, P.S. and Sebastian, S.A. (2002) Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds. Plant Physiology 128, 650-660.
9. Hoch, G., Peterbauer, T. and Richter, A. (1999) Purification and characterization of stachyose synthase from lentil (Lens culinaris) seeds: Galactopinitol and stachyose synthesis. Archives of Biochemistry and Biophysics 366, 75-81.
10. Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: Dependence on flatulence-producing oligosaccharides and cyclitols - review and survey. Seed Science Research 4, 385-405.
11. Horbowicz, M., Obendorf, R.L., McKersie, B.D. and Viands, D.R. (1995) Soluble saccharides and cyclitols in alfalfa (Medicago sativa L.) somatic embryos, leaflets, and mature seeds. Plant Science 109, 191-198.
12. Horbowicz, M., Brenac, P. and Obendorf, R.L. (1998) Fagopyritol B1, O-α-D-galactopyranosyl-(1→2)-D-chiro-inositol, a galactosyl cyclitol in maturing buckwheat seeds associated with desiccation tolerance. Planta 205, 1-11.
13. Kerr, P.S., Pearlstein, R.W., Schweiger, B.J., Becker-Manley, M.F. and Pierce, J.W. (inventors) (1997) Nucleotide sequences of galactinol synthase from zucchini and soybean. United States Patent Number 5648210, 15 July 1997.
14. Kuo, T.M., Lowell, C.A. and Nelsen, T.C. (1997a) Occurrence of pinitol in developing soybean seed tissues. Phytochemistry 45, 29-35.
15. Kuo, T.M., Lowell, C.A. and Smith, P.T. (1997b) Changes in soluble carbohydrates and enzymatic activities in maturing soybean seed tissues. Plant Science 125, 1-11.
16. Loewus, F.A. and Murthy, P.P.N. (2000) myo-Inositol metabolism in plants. Plant Science 150, 1-19.
17. Lowell, C.A. and Kuo, T.M. (1989) Oligosaccharide metabolism and accumulation in developing soybean seeds. Crop Science 29, 459-465.
18. Obendorf, R.L. (1997) Oligosaccharides and galactosyl cyclitols in seed desiccation tolerance (review update). Seed Science Research 7, 63-74.
19. Obendorf, R.L., Ashworth, E.N. and Rytko, G.T. (1980) Influence of seed maturation on germinability in soybean. Crop Science 20, 483-486.
20. Obendorf, R.L., Moon, H., Hildebrand, D.F., Torisky, R. and Collins, G.B. (1996) A comparison of pinitols in somatic and zygotic soybean embryos. Molecular and Cellular Biology of the Soybean 6, 40.
21. Obendorf, R.L., Dickerman, A.M., Pflum, T.M., Kacalanos, M.A. and Smith, M.E. (1998a) Drying rate alters soluble carbohydrates, desiccation tolerance, and subsequent seedling growth of soybean (Glycine max L. Merrill) zygotic embryos during in vitro maturation. Plant Science 132, 1-12.
22. Obendorf, R.L., Horbowicz, M., Dickerman, A.M., Brenac, P. and Smith, M.E. (1998b) Soluble oligosaccharides and galactosyl cyclitols in maturing soybean seeds in planta and in vitro. Crop Science 38, 78-84.
23. Odorcic, S. (2003) The anabolic and catabolic pathways of soluble α-galactosides in soybean (Glycine max (L.) Merrill) seeds. Senior Biology Research Honors Thesis, Cornell University, Ithaca, New York.
24. Odorcic, S. and Obendorf, R.L. (2003) Galactosyl cyclitol accumulation enhanced by substrate feeding of soybean embryos. pp. 51-60 in Nicolás, G.; Bradford, K.J.; Côme, D.; Pritchard, H. (Eds) The biology of seeds: Recent research advances. Wallingford, CABI Publishing.
25. Peterbauer, T. and Richter, A. (1998) Galactosylononitol and stachyose synthesis in seeds of adzuki bean: Purification and characterization of stachyose synthase. Plant Physiology 117, 165-172.
26. Peterbauer, T. and Richter, A. (2001) Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Science Research 11, 185-198.
27. Peterbauer, T., Mach, L., Mucha, J. and Richter, A. (2002a) Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis. Planta 215, 839-846.
28. Peterbauer, T., Mucha, J., Mach, L. and Richter, A. (2002b) Chain-elongation of raffinose in pea seeds: Isolation, characterization and molecular cloning of a multifunctional enzyme catalyzing the synthesis of stachyose and verbascose. Journal of Biological Chemistry 277, 194-200.
29. Saravitz, D.M., Pharr, D.M. and Carter, T.E. (1987) Galactinol synthase activity and soluble sugars in developing seeds of four soybean genotypes. Plant Physiology 83, 185-189.
30. Schweizer, T.F. and Horman, I. (1981) Purification and structure determination of three α-D-galactopyranosylcyclitols from soya beans. Carbohydrate Research 95, 61-71.
31. Shoemaker, R., Keim, P., Vodkin, L., Erpelding, J., Coryell, V., Khanna, A., Bolla, B., Marra, M., Hillier, L., Kucaba, T., Martin, J., Beck, C., Wylie, T., Underwood, K., Steptoe, M., Theising, B., Allen, M., Bowers, Y., Person, B., Swaller, T., Gibbons, M., Pape, D., Harvey, N., Schurk, R., Ritter, E., Kohn, S., Shin, T., Jackson, Y., Cardenas, M., McCann, R., Waterson, R. and Wilson, R. (1999) Public soybean EST project. (unpublished); GenBank BE33077; Genome Systems Clone ID: Gm-c1041-80 (5′), cDNA clone from mature senescing soybean leaf, Genome Systems Inc., 4633 World Parkway Circle, St. Louis, Missouri 63134.
32. Streeter, J.G., Lohnes, D.G. and Fioritto, R.J. (2001) Patterns of pinitol accumulation in soybean plants and relationships to drought tolerance. Plant, Cell and Environment 24, 429-438.
33. Volk, G.M. (1998) Plasmodesmatal formation and galactinol synthase expression in melon. PhD dissertation, Cornell University, Ithaca, New York, pp. 176-187.
34. Wanek, W. and Richter, A. (1997) Biosynthesis and accumulation of D-ononitol in Vigna umbellata in response to drought stress. Physiologia Plantarum 101, 416-424.
35. Whistler, R.L. and Durso, D.F. (1950) Chromatographic separation of sugars on charcoal. Journal of the American Chemical Society 72, 677-679.