Application of the measurement of oxidized pyridine dinucleotides with high-performance liquid chromatography-fluorescence detection to assay the uncoupled oxidation of NADPH by neuronal nitric oxide synthase

Analytical Biochemistry

Volumn 326, Issue 1, 2004, Pages 69-77

  Pálfi, Melinda ^a   Halász, Attila Sándor ^a   Tábi, Tamás ^a   Magyar, Kálmán ^a,b   Szöko, Éva ^a  

^a SEMMELWEIS UNIVERSITY   (Hungary)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

HPLC fluorescence detection; Nitric oxide synthase; Oxidized pyridine dinucleotides; Uncoupled reaction

Indexed keywords

ACETONE; ALKALINITY; AMINO ACIDS; ENZYMES; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NITRIC OXIDE; PYRIDINE;

CONDENSATION PRODUCT; DINUCLEOTIDES; FLUORESCENCE DERIVATIZATION; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-FLUORESCENCE DETECTION; HPLC-FLUORESCENCE DETECTION; NEURONAL NITRIC-OXIDE SYNTHASE (NNOS); NITRIC-OXIDE SYNTHASE; UNCOUPLED REACTION;

OXIDATION;

ACETONE; ACETOPHENONE; CARBONYL DERIVATIVE; CITRULLINE; DINUCLEOTIDE; FORMIC ACID; KETONE DERIVATIVE; NICOTINAMIDE; NITRIC OXIDE SYNTHASE; PYRIDINE DERIVATIVE; PYRIDINIUM DERIVATIVE;

ADDITION REACTION; ANALYTIC METHOD; ARTICLE; CHEMICAL REACTION; ENZYMATIC ASSAY; FLUORESCENCE; HEAT TREATMENT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; OXIDATION; POLYMERIZATION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SEPARATION TECHNIQUE; SPECTRAL SENSITIVITY;

EID: 0842303045     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.11.010     Document Type: Article

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