Thermostable DNA Polymerase from Thermus thermophilus B35: Preparation and Study of a Modified Form of the Enzyme with High Affinity to ddNTP

Biochemistry (Moscow)

Volumn 68, Issue 12, 2003, Pages 1307-1312

  Akishev, A G ^a   Rechkunova, N I ^b   Tomilova, J E ^c   Lebedeva, N A ^b   Lavrik, O I ^b   Degtyarev, S Kh ^a  

^a INSTITUTE OF MOLECULAR BIOLOGY AND BIOPHYSICS   (Russian Federation)

^b INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

^c SibEnzyme Res and Prod Association   (Russian Federation)

Author keywords

Dideoxynucleoside triphosphate; DNA sequencing; Site directed mutagenesis; Thermostable DNA polymerase

Indexed keywords

AMINO ACIDS; CATALYSIS; CHROMATOGRAPHIC ANALYSIS; ELECTROPHORESIS; ENZYMES; GENES; MOLECULAR STRUCTURE; YEAST;

DNA SEQUENCING; GENE ENCODING;

DNA;

THERMUS; THERMUS THERMOPHILUS;

DNA DIRECTED DNA POLYMERASE; DNA POLYMERASE, THERMUS THERMOPHILUS; NUCLEOSIDE; PRIMER DNA;

ARTICLE; AUTORADIOGRAPHY; CATALYSIS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; TEMPERATURE; THERMUS THERMOPHILUS;

AUTORADIOGRAPHY; BASE SEQUENCE; CATALYSIS; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEOSIDES; SEQUENCE ALIGNMENT; TEMPERATURE; THERMUS THERMOPHILUS;

EID: 0742320835     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BIRY.0000011651.66752.80     Document Type: Article

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