Thermostable DNA-polymerase from Thermus thermophilus B35: Isolation and characterization of some properties

Biochemistry (Moscow)

Volumn 63, Issue 11, 1998, Pages 1266-1270

  Rechkunova, N I ^a   Akishev, A G ^b   Lebedeva, N A ^c   Khodyreva, S N ^a   Deglyarev, S Kh ^b   Lavrik, O I ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

^b INSTITUTE OF MOLECULAR BIOLOGY AND BIOPHYSICS   (Russian Federation)

^c NOVOSIBIRSK STATE UNIVERSITY   (Russian Federation)

Author keywords

DNA amplification; Isolation; Properties; Thermostable DNA polymerase; Thermus

Indexed keywords

THERMUS; THERMUS THERMOPHILUS;

DIVALENT CATION; DNA DIRECTED DNA POLYMERASE; HYDROXYAPATITE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; CHROMATOGRAPHY; ENZYME STABILITY; ENZYMOLOGY; HEAT; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; THERMODYNAMICS; THERMUS THERMOPHILUS;

CATIONS, DIVALENT; CHROMATOGRAPHY; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; DNA-DIRECTED DNA POLYMERASE; DURAPATITE; ENZYME STABILITY; HEAT; KINETICS; MOLECULAR WEIGHT; THERMODYNAMICS; THERMUS THERMOPHILUS;

EID: 0032199401     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988) Science, 239, 487-491.
2. Innis, M. A., Myambo, K. B., Gelfand, D. H., and Brow, M. A. D. (1988) Proc. Natl. Acad. Sci. USA, 85, 9436-9440.
3. Bej, A. K., and Mahbubani, M. H. (1994) in PCR Technology: Current Innovation CRC Press Inc., pp. 219-237.
4. Carothers, A. M., Urlaub, G., Mucha, J., Grunberger, D., and Chasin, L. A. (1989) BioTechniques, 7, 494-499.
5. Kocher, T. D., and Wilson, A. C. (1991) in Essential Molecular Biology. A Practical Approach Pt. 2, Oxford University Press, Oxford, pp. 185-207.
6. Korolev, S., Nayal, M., Barnes, W. M., di Cera, E., and Waksman, G. (1995) Proc. Natl. Acad. Sci. USA, 92, 9264-9268.
7. Carballeira, N., Nazabal, M., Brito, J., and Garcia, O. (1990) BioTechniques 9, 276-281.
8. Myers, T. W., and Gelfand, D. H. (1991) Biochemistry, 30, 7661-7666.
9. Kaledin, A. S., Slyusarenko, A. G., and Gorodetsky, S. I. (1980) Biokhimiya, 45, 644-651.
10. Kaledin, A. S., Slyusarenko, A. G., and Gorodetsky, S. I. (1981) Biokhimiya, 46, 1576-1584.
11. Kaledin, A. S., Slyusarenko, A. G., and Gorodetsky, S. I. (1982) Biokhimiya, 47, 1785-1791.
12. Laemmli, U. K. (1992) Nature, 227, 680-685.
13. Lavrik, O. I., Nevinsky, G. A., Podust, V. N., and Khalabuda, O. V. (1990) Mol. Biol. (Moscow), 23, 388-399.
14. Lawyer, F. C., Stoffel, S., Saiki, R. K., Myambo, K., Drummond, R., and Gelfand, D. H. (1989) J. Biol. Chem., 264, 6427-6437.
15. Asakura, K., Komatsubara, H., Soga, S., Yomo, T., Oka, M., Emi, S., and Urabe, I. (1993) J. Ferment. Bioeng., 76, 265-269.
16. Elie, C., de Recondo, A. M., and Forterre, P. (1989) Eur. J. Biochem, 178, 619-626.
17. Sato, S., Nakasawa, K., and Shinomiya, T. (1980) J. Biochem, 88, 731-747.