Insertional inactivation of the lpxA gene involved in the biosynthesis of lipid A in Neisseria meningitidis resulted in lpxA/lpxA::aph-3′ heterodiploids

FEMS Microbiology Letters

Volumn 226, Issue 1, 2003, Pages 51-56

  Zarantonelli, Maria Leticia ^a   Carlier, Jean Philippe ^a   Alonso, Jean Michel ^a   Taha, Muhamed Kheir ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

Endotoxin; Gene inactivation; Heterodiploid; Lipid A; Lipooligosaccharide; LOS, lipooligosaccharide; lpxA; Neisseria meningitidis; ORF, open reading frame; PAGE, polyacrylamide gel electrophoresis

Indexed keywords

BACTERIAL PROTEIN; KANAMYCIN; LIPID A; LIPOOLIGOSACCHARIDE; PROTEIN LPXA; UNCLASSIFIED DRUG; ADENOSINE DIPHOSPHATE L GLYCERO D MANNOHEPTOSE 6 EPIMERASE; ADP-L-GLYCERO-D-MANNOHEPTOSE-6-EPIMERASE; EPIMERASE; LIPID LINKED OLIGOSACCHARIDES; LIPID-LINKED OLIGOSACCHARIDES; LIPOPOLYSACCHARIDE;

ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIUM MUTANT; DIPLOIDY; GENE CASSETTE; GENE FREQUENCY; GENE INACTIVATION; GENE INSERTION; LIPOGENESIS; NEISSERIA MENINGITIDIS; NONHUMAN; PRIORITY JOURNAL; WILD TYPE; ANTIBIOTIC RESISTANCE; BIOSYNTHESIS; ESSENTIAL GENE; GAS CHROMATOGRAPHY; GENE DUPLICATION; GENE EXPRESSION REGULATION; GENE ORDER; GENETICS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SOUTHWESTERN BLOTTING;

INSERTION SEQUENCES; NEGIBACTERIA; NEISSERIA; NEISSERIA MENINGITIDIS;

BLOTTING, SOUTHWESTERN; CARBOHYDRATE EPIMERASES; CHROMATOGRAPHY, GAS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE DUPLICATION; GENE ORDER; GENES, BACTERIAL; GENES, ESSENTIAL; KANAMYCIN RESISTANCE; LIPID A; LIPOPOLYSACCHARIDES; MUTAGENESIS, INSERTIONAL; NEISSERIA MENINGITIDIS; POLYMERASE CHAIN REACTION;

EID: 0642369580     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00558-5     Document Type: Article

References (19)

1. Kahler C.M., Stephens D.S. Genetic basis for biosynthesis, structure, and function of meningococcal lipooligosaccharide (endotoxin). Crit. Rev. Microbiol. 24:1998;281-334.
2. Steeghs L., Jennings M.P., Poolman J.T., van der Ley P. Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis. Gene. 190:1997;263-270.
3. Galloway S.M., Raetz C.R. A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis. J. Biol. Chem. 265:1990;6394-6402.
4. Steeghs L., den Hartog R., den Boer A., Zomer B., Roholl P., van der Ley P. Meningitis bacterium is viable without endotoxin. Nature. 392:1998;449-450.
5. Steeghs L., de Cock H., Evers E., Zomer B., Tommassen J., van der Ley P. Outer membrane composition of a lipopolysaccharide-deficient Neisseria meningitidis mutant. EMBO J. 20:2000;6937-6945.
6. Albiger B., Johansson L., Jonsson A.-B. Lipooligosaccharide-deficient Neisseria meningitidis shows altered pilus-associated characteristics. Infect. Immun. 71:2003;155-162.
7. Taha M.-K. Neisseria meningitidis induces the expression of the TNF-α gene in endothelial cells. Cytokine. 12:2000;21-25.
8. Kellogg D.S., Peacock W.L., Deacon W.E., Brown L., Pirkle C.I. Neisseria gonorrhoeae. I. Virulence genetically linked to clonal variation. J. Bacteriol. 85:1963;1274-1279.
9. Taha M.K., So M., Seifert H.S., Billyard E., Marchal C. Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control. EMBO J. 7:1988;4367-4378.
10. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Tettelin H., Saunders N.J., Heidelberg J., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., et al. Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science. 287:2000;1809-1815.
12. Meyer T.F., Mlawer N., So M. Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement. Cell. 30:1982;45-52.
13. Hitchcock P.J., Brown T.M. Morphological heterogeneity among Salmonella lipooligosaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:1983;269-277.
14. Tsai C.M., Frasch C.E. A sensitive silver stain for detecting lipopolysaccharide in polyacrylamide gels. Anal. Biochem. 119:1982;115-119.
15. Ghanem F.M., Ridpath A.C., Moore W.E.C., Moore L.V.H. Identification of Clostridium botulinum, Clostridium argentinense, and related organisms by cellular fatty acid analysis. J. Clin. Microbiol. 29:1991;1114-1124.
16. Coleman W.G. The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6- epimerase, an enzyme required for lipopolysaccharide core biosynthesis. J. Biol. Chem. 258:1983;1985-1990.
17. Taha M.-K., Giorgini D., Nassif X. The pilA regulatory gene modulates the pilus-mediated adhesion of Neisseria meningitidis by controlling the transcription of pilC1. Mol. Microbiol. 19:1996;1073-1084.
18. Horii T., Ozawa N., Ogawa T., Ogawa H. Inhibitory effects of N- and C-terminal truncated Escherichia coli recA gene products on functions of the wild-type recA gene. J. Mol. Biol. 223:1992;105-114.
19. Ogawa T., Wabiko H., Tsurimoto T., Horii T., Masukata H., Ogawa H. Characteristic of purified RecA protein and the regulation of its synthesis in vivo. Cold Spring Harbor Symp. Quant. Biol. 2:1979;909-915.