Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis

Gene

Volumn 190, Issue 2, 1997, Pages 263-270

  Steeghs, Liana ^a   Jennings, Michael P ^b   Poolman, Jan T ^a   Van Der Ley, Peter ^a  

^a NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^b GRIFFITH UNIVERSITY   (Australia)

Author keywords

Acyltransferases; Endotoxin; Gram negative bacteria; Hexapeptide repeat; Lipopolysaccharides; Outer membrane

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; LIPID A;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE CLUSTER; GENE ISOLATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC COMPLEMENTATION; LIPOGENESIS; MOLECULAR CLONING; NEISSERIA MENINGITIDIS; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; GRAM-NEGATIVE BACTERIA; HYDRO-LYASES; LIPID A; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; NEISSERIA MENINGITIDIS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0030987731     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00005-X     Document Type: Article

References (22)

1. Allen, S.W., Senti-Willis, A. and Maloy, S.R. (1993) DNA sequence of the putA gene from Salmonella typhimurium: a bifunctional membrane-associated dehydrogenase that binds DNA. Nucleic Acids Res. 21, 1676.
2. Anderson, M.S and Raetz, C.R.H (1987) Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine. J. Biol. Chem. 262, 5159-5169.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.M., Seidman, J.G., Smith, J.A. and Struhl, K. (1989) Current Protocols in Molecular Biology. Greene, New York.
4. Coleman, J. and Raetz, C.R.H. (1988) First committed step of lipid A biosynthesis in Escherichia coli: Sequence of the lpxA gene. J. Bacteriol. 170, 1268-1274.
5. Crowell, D.N., Reznikoff, W.S. and Raetz, C.R.H. (1987) Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase. J. Bacteriol. 169, 5727-5734.
6. Dicker, I.B. and Seetharam, S. (1991) Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173, 334-344.
7. Fleischmann, R.D. et al. (1995) Whole-genome random sequencing and assembly of Haemophilus influenza? Rd. Science 269, 496-512.
8. Hirvas, L., Koski, P. and Vaara, M. (1991) Identification and sequence analysis of the gene mutated in the conditionally lethal outer membrane permeability mutant SSC of Salmonella typhimurium. EMBO J. 10, 1017-1023.
9. Jennings, M.P., Bisercic, M., Dunn, K.L.R., Virji, M., Martin, A., Wilks, K.E., Richards, J.C. and Moxon, E.R. (1995) Cloning and molecular analysis of the lsi-1 (rfaF) gene of Neisseria meningitidis which encodes a heptosyl-2-transferase involved in LPS biosynthesis: evaluation of surface exposed carbohydrates in LPS mediated toxicity for human endothelial cells. Microb. Pathogen. 19, 391-407.
10. Kelly, T.M., Stachula, S.A., Raetz, C.R.H. and Anderson, M.S. (1993) The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. J. Biol. Chem. 268, 19866-19874.
11. Kulshin, V.A., Zähringer, U., Lindner, B., Frasch C.E., Tsai, C., Dimitriev, A. and Rietschel, E.T. (1992) Structural characterization of the lipid A component of pathogenic Neisseria meningitidis. J. Bacteriol. 174, 1793-1800.
12. Lesse, A.J., Campagnari, A.A., Bittner, W.E. and Apicella, M.A. (1990) Increased resolution of lipopolysaccharides and lipooligosaccharides utilising tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Immunol. Methods 126, 109-117.
13. Mohan, S., Kelly, T.M., Eveland, S.S., Raetz, C.R.H. and Anderson, M.S. (1994) An Escherichia coli gene (FabZ) encoding (3R)-hydroxymyristoyl acyl carrier protein dehydrase. J. Biol. Chem. 269, 32896-32903.
14. Raetz, C.R.H. and Roderick, S.L. (1995) A left-handed parallel β helix in the structure of UDP-N-acetylglucosamine acyltransferase. Science 270, 997-1000.
15. Roy, A.M. and Coleman, J. (1994) Mutations in firA, encoding the second acyltransferase in lipopolysaccharide biosynthesis, affect multiple steps in lipopolysaccharide biosynthesis. J. Bacteriol. 176, 1639-1646.
16. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Shaw, E.I. and Wood, D.O. (1994) Characterization of a Rickettsia rickettsii DNA fragment analogous to the firA-ORF17-lpxA region of Escherichia coli. Gene 140, 109-113.
18. Tsai, C.M. and Frasch, C.E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119, 115-119.
19. Verheul, A.F.M., Snippe, H. and Poolman, J.T. (1993) Meningococcal lipopolysaccharides: Virulence factor and potential vaccine component. Microbiol. Rev. 57, 34-49.
20. Vuorio, R., Härkönen, T., Tolvanen, M. and Vaara, M. (1994) The novel hexapeptide motif found in the acyltransferases LpxA and LpxD of lipid A biosynthesis is conserved in various bacteria. FEBS Lett. 337, 289-292.
21. Williamson, J.M., Anderson, M.S. and Raetz, C.R.H. (1991) Acylacyl carrier protein specificity of UDP-GlcNac acyltransferase from Gram-negative bacteria: relationship to lipid A structure. J. Bacteriol. 173, 3591-3596.
22. Xia, M., Zhu, Y., Cao, X., You, L. and Chen, Z. (1995) Cloning, sequencing and analysis of a gene encoding Escherichia coli proline dehydrogenase. FEMS Microbiol. Lett. 127, 235-242.