Detection of Cryptosporidium parvum in secondary effluents using a most probable number-polymerase chain reaction assay

Water Environment Research

Volumn 75, Issue 4, 2003, Pages 292-299

  Tsuchihashi, Ryujiro ^a   Loge, Frank J ^b   Darby, Jeannie L ^a  

^a University of California   (United States)

^b Washington State University   (United States)

Author keywords

Cryptosporidium parvum; Particle association; Polymerase chain reaction; Wastewater treatment

Indexed keywords

CELLS; CONCENTRATION (PROCESS); ENZYME KINETICS; ENZYMES; EXTRACTION; NUCLEIC ACIDS; POISSON DISTRIBUTION; PURIFICATION; REACTION KINETICS;

CRYSTOSPORODIUM PARVUM; NUCLEIC ACID EXTRACTS; SECONDARY EFFLUENTS;

EFFLUENTS;

NUCLEIC ACID; PHOSPHATE BUFFERED SALINE;

ACTIVATED SLUDGE; DETECTION METHOD; MICROBIAL POLLUTANT; WASTEWATER TREATMENT;

ACTIVATED SLUDGE; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; EFFLUENT; EXTRACTION; MEAN RESIDENCE TIME; MICROORGANISM DETECTION; MOST PROBABLE NUMBER; NONHUMAN; OOCYST; PARTICLE SIZE; POISSON DISTRIBUTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WASTE WATER MANAGEMENT; ANIMAL; ENTEROBACTERIACEAE; ISOLATION AND PURIFICATION; METHODOLOGY; PARASITOLOGY; SEWAGE;

CRYPTOSPORIDIUM PARVUM;

ANIMALS; CRYPTOSPORIDIUM PARVUM; ENTEROBACTERIACEAE; OOCYSTS; POISSON DISTRIBUTION; POLYMERASE CHAIN REACTION; SEWAGE;

EID: 0642307074     PISSN: 10614303     EISSN: None     Source Type: Journal    
DOI: 10.2175/106143003X141097     Document Type: Article

References (28)

1. American Public Health Association; American Water Works Association; Water Environment Federation (1998) Standard Methods for the Examination of Water and Wastewater, 20th ed.; Washington, D.C.
2. Bisogni, J. J. J.; Lawrence, A. M. (1971) Relationships Between Biological Solids Retention Time, and Settling Characteristics of Activated Sludge. Water Res., 5, 753.
3. Bukhari, Z.; Smith, H. V.; Sykes, N.; Humphreys, S. W.; Paton, C. A.; Girdwood, R. W. A.; Fricker, C. R. (1997) Occurrence of Cryptosporidium spp. Oocysts and Giardia spp. Cysts in Sewage Influents and Effluents from Treatment Plants in England. Water Sci. Technol., 35 (11/12), 385.
4. Chao, A. C.; Keinath, T. M. (1979) Influence of Process Loading Intensity on Sludge Clarification and Thickening Characteristics. Water Res., 13, 1213.
5. Chauret, C.; Springthorpe, S.; Sattar, S. (1999) Fate of Cryptosporidium Oocysts, Giardia Cysts, and Microbial Indicators During Wastewater Treatmentand Anaerobic Sludge Digestion. Can. J. Microbiol., 45, 257.
6. Cullen, D. W.; Hirsch, P. R. (1998) Simple and Rapid Method for Direct Extraction of Microbial DNA from Soil for PCR. Soil Biol. Biochem., 30, 983.
7. Degrange, V.; Bardin, R. (1995) Detection and Counting of Nitrobacter Populations in Soil by PCR. Appl. Environ. Microbiol., 61, 2093.
8. Deng, M. Q.; Cliver, D. O.; Mariam, T. W. (1997) Immunomagnetic Capture PCR to Detect Viable Cryptosporidium parvum Oocysts from Environmental Samples. Appl. Environ. Microbiol., 63, 3134.
9. Emerick, R. W.; Loge, F. J.; Thompson, D. E.; Darby, J. L. (1999) Factors Influencing UV Disinfection Performance-Part II: Association of Coliform Bacteria with Wastewater Particles. Water Environ. Res., 71, 1178.
10. Enriquez, V.; Vinluan, E.; Rose, J. B.; Gerba, C. P. (1994) Occurrence of Cryptosporidium and Giardia in Secondary Treated Wastewater. Abstracts Gen. Meeting Am. Soc. Microbiol., 94, 425.
11. Johnson, D. W.; Pieniazek, N. J.; Griffin, D. W.; Misener, L.; Rose, J. B. (1995) Development of a PCR Protocol for Sensitive Detection of Cryptosporidium Oocysts in Water Samples. Appl Environ. Microbiol., 61, 3849.
12. Kaucner, C.; Stinear, T. (1998) Sensitive and Rapid Detection of Viable Giardia Cysts and Cryptosporidium parvum Oocysts in Large-Volume Water Samples with Wound Fiberglass Cartridge Filters and Reverse Transcription-PCR. Appl. Environ. Microbiol, 64, 1743.
13. Loge, F. J.; Emerick, R. W.; Ginn, T. R.; Darby, J. L. (2002) Association of Coliform Bacteria with Wastewater Particles; Impact of Operational Parameters of the Activated Sludge Process. Water Res., 36, 41.
14. Madore, M. S.; Rose, J. B.; Gerba, C. P.; Arrowood, M. J.; Sterling, C. R. (1987) Occurrence of Cryptosporidium Oocysts in Sewage Effluents and Selected Surface Waters. J. Parasit., 73, 702.
15. Mantynen, V.; Niemela, S.; Kaijalainen, S.; Pirhonen, T.; Lindstrom, K. (1997) MPN-PCR-Quantification Method for Staphylococcal Enterotoxin C1 Gene from Fresh Cheese. Int. J. Food Microbiol., 36, 135.
16. Mayer, C. L.; Palmer, C. J. (1996) Evaluation of PCR, Nested PCR, and Fluorescent Antibodies for Detection of Giardia and Cryptosporidium Species in Wastewater. Appl. Environ. Microbiol., 62, 2081.
17. Mayer, C. L.; Tsai, Y.; Palmer, C. J. (1995) The Use of PCR to Monitor the Decrease of Giardia and Cryptosporidium through the Wastewater Treatment Process. Abstracts Gen. Meeting Am. Soc. Microbiol., 95, 443.
18. Medema, G. J.; Schets, F. M.; Teunis, P. F. M.; Havelaar, A. H. (1998) Sedimentation of Free and Attached Cryptosporidium Oocysts and Giardia Cysts in Water. Appl. Environ. Microbiol., 64, 4460.
19. Miller, D. N.; Bryant, J. E.; Madsen, E. L.; Ghiorse, W. C. (1999) Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Sediment Samples. Appl. Environ. Microbiol, 65, 4715.
20. Roach, P. D.; Olson, M. E.; Whitley, G.; Wallis, P. M. (1993) Waterborne Giardia Cysts and Cryptosporidium Oocysts in the Yukon, Canada. Appl. Environ. Microbiol., 59, 67.
21. Robertson, L. J.; Paton, C. A.; Campbell, A. T.; Smith, P. G.; Jackson. M. H.; Gilmour, R. A.; Black, S. E.; Stevenson, D. A.; Smith, H. V. (2000) Giardia Cysts and Cryptosporidium Oocysts at Sewage Treatment Works in Scotland, UK. Water Res., 34, 2310.
22. Rochelle, P. A.; De Leon, R.; Stewart, M. H.; Wolfe, R. L. (1997) Comparison of Primers and Optimization of PCR Conditions for Detection of Cryptosporidium parvum and Giardia lamblia in Water. Appl. Environ, Microbiol., 63, 106.
23. Rose, J. B. (1988) Occurrence and Significance of Cryptosporidium in Water. J.-Am. Water Works Assoc., 82 (2), 53.
24. Rose, J. B.; Cifrino, A.; Madore, M. S.; Gerba, C. P.; Sterling; C. R.; Arrowood, M. J. (1986) Detection of Cryptosporidium from Wastewater and Freshwater Environments. Water Sci. Technol., 18 (10), 233.
25. Tchobanoglous, G.; Schroeder, E. D. (1987) Water Quality; Addison-Wesley: Reading, Massachusetts.
26. U.S. Environmental Protection Agency (1999) Method 1622: Cryptosporidium in Water by Filtration/TMS/IFA; Washington, D.C.
27. Yoshinaka, T.; Yoshimizu, M.; Ezura, Y. (1999) A Comparison of Filter Capture and Precultivation for Quantitative Detection of Infectious Hematopoietic Necrosis Virus (IHNV) by Using Polymerase Chain Reaction (PCR) Combined with the Most Probable Number (MPN) Method. J. Aquat. Anim. Health, 11, 154.
28. Zuckerman, U.; Gold, D.; Shelef, G.; Armon, R. (1997) The Presence of Giardia and Cryptosporidium in Surface Waters and Effluents in Israel. Water Sci. Technol., 35 (11/12), 381.