Effect of ethanol on cell growth and cholesterol metabolism in cultured Hep G2 cells

Biochemistry and Cell Biology

Volumn 81, Issue 6, 2003, Pages 379-386

  Polo, Mónica P ^a   De Bravo, Margarita G ^a,b   De Alaniz, María J T ^a  

^a INSTITUTO DE INVESTIGACIONES BIOQUÍMICAS DE LA PLATA INIBIOLP   (Argentina)

^b Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

Author keywords

Cholesterol; Ethanol; Hepatoma cells; HMG CoA reductase; Lipid metabolism

Indexed keywords

CHOLESTEROL; ENZYME INHIBITION; ENZYMES; ETHANOL; METABOLISM;

CELL GROWTH;

CELLS;

3 HYDROXY 3 METHYLGLUTARYL COENZYME A; ALCOHOL; CHOLESTEROL; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; MEVALONIC ACID;

ARTICLE; CELL GROWTH; CELL STRAIN HEPG2; CHOLESTEROL BLOOD LEVEL; CHOLESTEROL METABOLISM; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; EVOKED RESPONSE; GROWTH RATE; HEPATOMA CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; INCUBATION TIME; LIVER CELL CARCINOMA; REGULATORY MECHANISM;

CARCINOMA, HEPATOCELLULAR; CELL DIVISION; CHOLESTEROL; ENZYME INHIBITORS; ETHANOL; HUMANS; HYDROXYMETHYLGLUTARYL COA REDUCTASES; ISOTOPE LABELING; LIVER NEOPLASMS; MEVALONIC ACID; TUMOR CELLS, CULTURED;

EID: 0442307428     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o03-066     Document Type: Article

References (38)

1. Abrams, M.A., and Cooper, C. 1976. Mechanism of increased hepatic uptake of unesterified fatty acid from serum of ethanol-treated rats. Biochem. J. 156: 47-54.
2. Angeletti, C., and Alaniz, M.J.T. de. 1995. Fatty acid uptake and metabolism in Hep G2 human-hepatoma cells. Mol. Cell. Biochem. 143: 99-105.
3. Angeletti, C., and Alaniz, M.J.T. de. 1996. Effect of ethanol on glycerolipid and fatty acid metabolism in Hep G2 human-hepatoma cells. Acta Physiol. Pharmacol. Ther. Latinoam. 42: 57-69.
4. Avogaro, P., and Cazzolato, G. 1975. Changes in the composition and physico-chemical characteristics of serum lipoproteins during ethanol-induced lipaemia in alcoholic subjects. Metabolism, 24: 1231-1242.
5. Bailey, J.M., and Dunbar, L.M. 1971. Lipid metabolism in cultured cells: growth of tumor cells deficient in essential fatty acids. Cancer Res. 31: 91-97.
6. Baraona, E., and Lieber, C.S. 1979. Effects of ethanol on lipid metabolism. J. Lipid Res. 20: 289-315.
7. Baraona, E., and Lieber, C.S. 1998. Alcohol and lipids. In Recent developments in alcoholism. Vol. 14. Edited by M. Galanter. Plenum Publishing Corp., New York. pp. 97-134.
8. Barboriak, J.J. 1984. Alcohol, lipids and heart disease. Alcohol, 1: 341-345.
9. Baskin, G., Henderson, G.I., and Schenker, S. 1988. Ethanol and hepatic regeneration. Hepatology, 8: 408-411.
10. Benoist, F., and Grand-Perret, T. 1995. A sensitive method to analyze in vitro secretion of lipoproteins: distribution of apolipoproteins is modulated by oleic acid in HepG2 cells. J. Lipid Res. 36: 2243-2250.
11. Brown, M.S., Goldstein, J.L., and Dietschy, J.M. 1979. Active and inactive forms of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver of the rat. Comparison with the rate of cholesterol synthesis in different physiological states. J. Biol. Chem. 254: 5144-5149.
12. Cameron, R.G., Neuman, M.G., Shear, N.H., Katz, G., Bellentani, S., and Tiribelli, C. 1998. Modulation of liver-specific cellular response to ethanol in vitro in Hep G2 cells. Toxicol. In Vitro, 12: 111-122.
13. Cavenee, W.K., Chen, H.W., and Kandutsch, A.A. 1981. Regulation of cholesterol biosynthesis in enucleated cells. J. Biol. Chem. 256: 2675-2681.
14. Dashti, N., Franklin, F.A., and Abrahamson, D.R. 1996. Effect of ethanol on the synthesis and secretion of apoA-I- and apoB-containing lipoproteins in HepG2 cells. J. Lipid Res. 37: 810-824.
15. Frohlich, J.J. 1996. Effects of alcohol on plasma lipoprotein metabolism. Clin. Chim. Acta, 246: 39-49.
16. Goldstein, J.L., and Brown, M.S. 1990. Regulation of mevalonate pathway. Nature (Lond.), 343: 425-430.
17. Higgins, P.J. 1987. Cell cycle phase-specific perturbation of hepatic tumor cell growth kinetics during short-term in vitro exposure to ethanol. Alcohol Clin. Exp. Res. 11: 550-555.
18. Higgins, P.J., and Borenfreud, E. 1986a. Alterations in growth rate and cell cycle kinetics of rat liver tumor cells cultured in ethanol-containing medium. In vitro model of proliferative restriction in response to ethanol exposure. Biochem. Pharmacol. 35: 3857-3862.
19. Higgins, P.J., and Borenfreud, E. 1986b. Incubation of rat hepatic tumor cells with ethanol and acetaldehyde in vitro: effects on growth rate, albumin secretion and cellular protein content. Digestion, 34: 161-168.
20. Jaen, M.C. 1971 Direct blood injection method for gas chromatographic determination of alcohol and other volatiles compounds. Clin. Chem. 17: 82-85.
21. Jauregui, H.O., Hayner, N.T., Driscoll, J.L., Williams-Holland, R., Lipsky, M.H., and Galletti, P.M. 1981. Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes freshly isolated cells, cell suspensions, and primary monolayer cultures. In Vitro, 17: 1100-1110.
22. Levitt, M.D., Levitt, D.G., Furne, J., and DeMaster, E.G. 1994. Can liver account for first-pass metabolism of ethanol in the rat? Am. J. Physiol. 267: G452-G457.
23. Lieber, C.S. 1978. Pathogenesis and early diagnosis of alcoholic liver injury. Pathogenesis and early diagnosis of alcoholic liver injury. N. Engl. J. Med. 298: 888-893.
24. Lieber, C.S. 1994. Alcohol and the liver: 1994 update. Gastroenterology, 106: 1085-1105.
25. Musanti, R., Giorgini, L., Lovisolo, P.P., Pirillo, A., Chiari, A., and Ghiselli, G. 1996. Inhibition of acyl-CoA: cholesterol acyltransferase decreases apolipoprotein B-100-containing lipoprotein secretion from HepG2 cells. J. Lipid Res. 37: 1-14.
26. Neuman, M.G., Cameron, R.G., Shear, N.H., Bellentani, S., and Tiribelli, C. 1995. Effect of taurourso-deoxycholate and ursodeoxycholic acid on ethanol-induced cell injuries in human Hep G2 cell line. Gastroenterology, 109: 555-563.
27. Nikkila, E.A., and Ojala, K. 1963. Role of hepatic L-α- glycerophosphate and triglyceride synthesis in the production of fatty liver by ethanol. Proc. Soc. Exp. Biol. Med. 113: 814-817.
28. Ontko, J.A. 1973. Effects of ethanol on the metabolism of free fatty acids in isolated liver cells. J. Lipid Res. 14: 78-86.
29. Patterson, M.K. 1979. Measurement of growth and viability of cells in culture. Methods Enzymol. 58: 141-152.
30. Polokoff, M.A., Iwahashi, M., and Simon, F.R. 1983. Ethanol treatment increases triacylglycerol and cholesteryl ester content of cultured hepatoma cells. J. Lipid Res. 24: 1030-1038.
31. Richter, A., Sanford, R.R., and Evans, V. 1972. Influence of oxygen and culture media on plating efficiency of some mammalian tissue cells. J. Natl. Cancer Inst. 49: 1705-1712.
32. Sabesin, M.D. 1981. Lipid and lipoprotein abnormalities in alcoholic liver disease. Circulation, 64(Suppl. III): 72-84.
33. Sambrook, J., and Russell, D.W. 2001. Measurement of radioactivity in nucleic acids. Appendix 8. Commonly used techniques in molecular cloning. In Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Plainview, N.Y. pp. A8.25-A8.26.
34. Saucier, S.E., and Kandutsch, A.A. 1979. Inactive 3-hydroxy-3- methylglutaryl-coenzyme A reductase in broken cell preparations of various mammalian tissues and cell cultures. Biochim. Biophys. Acta, 572: 541-556.
35. Shapiro, D.J., Nordstrom, J.L., Mitschelen, J.J., Rodwell, V.W., and Schimke, R.T. 1974. Biochim. Biophys. Acta, 370: 369-377.
36. Steinberg, D., Pearson, T.A., and Kuller, L.H. 1991. Alcohol and atherosclerosis. Ann. Intern. Med. 114: 967-976.
37. Taskinen, M.R., Nikkila, E.A., Valimaki, M., Sane, T., Kuusi, T., Kesaniemi, A., and Ylikahri, R. 1987. Alcohol-induced changes in serum lipoproteins and in their metabolism. Am. Heart J. 113: 458-464.
38. Visioli, F., Monti, S., Colombo, C., and Galli, C. 1998. Ethanol enhances cholesterol synthesis and secretion in human hepatomal cells. Alcohol, 15: 299-303.