Expression Profiles of Polyhydroxyalkanoate Synthesis-Related Genes in Paracoccus denitrificans

Journal of Bioscience and Bioengineering

Volumn 97, Issue 1, 2004, Pages 45-53

  Kojima, Takaaki ^a   Nishiyama, Tatsuaki ^a   Maehara, Akira ^b   Ueda, Shunsaku ^c   Nakano, Hideo ^a   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b MITSUBISHI GAS CHEMICAL COMPANY INC   (Japan)

^c UTSUNOMIYA UNIVERSITY   (Japan)

Author keywords

Acetyl coenzyme A; Paracoccus denitrificans; Polyhydroxyalkanoate

Indexed keywords

BACTERIA; CLONING; DENITRIFICATION; GENES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ORGANIC COMPOUNDS; PROTEINS; RNA; SYNTHESIS (CHEMICAL);

REGULATION MECHANISM; TRANSLATIONAL LEVELS;

BIOMEDICAL ENGINEERING;

ACETYL COENZYME A; MESSENGER RNA; NITROGEN; POLYHYDROXYALKANOIC ACID; PROTEIN PHAA; PROTEIN PHAB; PROTEIN PHAC; PROTEIN PHAP; PROTEIN PHAR; PROTEIN PHAZ; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; CONTROLLED STUDY; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METHYLOTROPHIC BACTERIUM; MOLECULAR CLONING; NONHUMAN; PARACOCCUS DENITRIFICANS; POLYMERIZATION; PROTEIN CONTENT; REGULATORY MECHANISM; RNA TRANSLATION;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PARACOCCUS DENITRIFICANS;

EID: 0442297959     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(04)70164-4     Document Type: Review

References (23)

1. Anderson, A. J. and Dawes, E. A.: Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev., 54, 450-472 (1990).
2. Madison, L. L. and Huisman, G. W.: Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev., 63, 21-53 (1999).
3. Yamane, T., Chen, X. F., and Ueda, S.: Polyhydroxyalkanoate synthesis from alcohols during the growth of Paracoccus denitrificans. FEMS Microbiol. Lett., 135, 207-211 (1996).
4. Yamane, T., Chen, X. F., and Ueda, S.: Growth-associated production of poly(3-hydroxyvalerate) from n-pentanol by a methylotrophic bacterium, Paracoccus denitrificans. Appl. Environ. Microbiol., 62, 380-384 (1996).
5. Ueda, S., Matsumoto, S., Takagi, A., and Yamane, T.: Synthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from methanol and n-amyl alcohol by the methylotrophic bacteria Paracoccus denitrificans and Methylobacterium extorquens. Appl. Environ. Microbiol., 58, 3574-3579 (1992).
6. Yabutani, T., Maehara, A., and Yamane, T.: Analysis of β-ketothiolase and acetoacetyl-CoA reductase genes of a methylotrophic bacterium, Paracoccus denitrificans, and their expression in Escherichia coli. FEMS Microbiol. Lett., 133, 85-90 (1999).
7. Ueda, S., Yabutani, T., Maehara, A., and Yamane, T.: Molecular analysis of the poly(3-hydroxyalkanoate) synthase gene from a methylotrophic bacterium, Paracoccus denitrificans. J. Bacteriol., 178, 774-779 (1996).
8. Maehara, A., Ueda, S., Nakano, H., and Yamane, T.: Analysis of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitrificans. J. Bacteriol., 177, 2425-2435 (1999).
9. Steinbüchel, A., Aerts, K., Babel, W., Föllmer, C., Liebergesell, M., Madkour, M. H., Mayer, F., Pieper-Fürst, U., Pries, A., Valentin, H. E., and Wieczorek, R.: Consideration on the structure and biochemistry of bacterial polyhydroxyalkanoic acid inclusions. Can. J. Microbiol., 41, 94-105 (1995).
10. Pieper-Fürst, U., Madkour, M. H., Mayer, F., and Steinbüchel, A.: Purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in Rhodococcus ruber. J. Bacteriol., 176, 4328-4337 (1994).
11. Wieczorek, R., Pries, A., Steinbüchel, A., and Mayer, F.: Analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in Alcaligenes eutrophus. J. Bacteriol., 177, 2425-2435 (1995).
12. Maehara, A., Doi, Y., Nishiyama, T., Takagi, Y., Ueda, S., Nakano, H., and Yamane, T.: PhaR, a protein of unknown function conserved among short-chain-length polyhydroxyalkanoic acids producing bacteria, is a DNA-binding protein and represses Paracoccus denitrificans phaP expression in vitro. FEMS Microbiol. Lett., 200, 9-15 (2001).
13. Bullock, W. O., Fernandez, J. M., and Stuart, J. M.: XL-1 Blue: a high efficiency plasmid transforming recA Escherichia coli strain with betagalactosidase selection. BioTechniques, 5, 376-379 (1987).
14. Maehara, A., Ikai, K., Ueda, S., and Yamane, T.: Gene dosage effects on polyhydroxyalkanoates synthesis from n-alcohols in Paracoccus denitrificans. Biotechnol. Bioeng., 60, 61-69 (1998).
15. Sambrook, J., Fritsch, E. F., and Maniatis, T.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
16. Yan, W., Jian-Ming, L., Richmond, C., Blattner, F., Rafalski, J., and Larossa, R.: High-density microarray-mediated gene expression profiling of Escherichia coli. J. Bacteriol., 183, 545-556 (2001).
17. Gao, D., Maehara, A., Yamane, T., and Ueda, S.: Identification of the intracellular polyhydroxyalkanoate depolymerase gene of Paracoccus denitrificans and some properties of the gene product. FEMS Microbiol. Lett., 196, 159-164 (2001).
18. Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
19. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
20. Burnette, W. N.: "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem., 112, 195-203 (1981).
21. MacGregor, L. C. and Matschinsky, F. M.: An enzymatic fluorimetric assay for myo-inositol. Anal. Biochem., 141, 382-389 (1984).
22. King, M. T., Reiss, P. D., and Cornell, N. W.: Determination of short-chain coenzyme A compounds by reverse-phase high-performance liquid chromatography. Methods Enzymol., 166, 70-79 (1988).
23. Maehara, A., Taguchi, S., Nishiyama, T., Yamane, T., and Doi, Y.: A repressor protein, PhaR, regulates polyhydroxyalkanoate (PHA) synthesis via its direct interaction with PHA. J. Bacteriol., 184, 3992-4002 (2002).