Gene dosage effects on polyhydroxyalkanoates synthesis from n-alcohols in Paracoccus denitrificans

Biotechnology and Bioengineering

Volumn 60, Issue 1, 1998, Pages 61-69

  Maehara, Akira ^a   Ikai, Kaoru ^a   Ueda, Shunsaku ^b   Yamane, Tsuneo ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b UTSUNOMIYA UNIVERSITY   (Japan)

Author keywords

Biodegradable plastics; Fed batch culture; Methylotroph; Molecular weight; Polyhydroxyalkanoate

Indexed keywords

BACTERIA; BATCH CELL CULTURE; BIOMASS; BIOSYNTHESIS; COMPOSITION EFFECTS; ENZYMES; ETHANOL; MOLECULAR WEIGHT; OXIDATION;

FED-BATCH CELL CULTURE; PENTANOL; POLYHYDROXYALKANOATES;

CLONING;

POLYHYDROXYALKANOIC ACID;

ARTICLE; BACTERIAL GENE; FATTY ACID OXIDATION; GENE DOSAGE; MOLECULAR WEIGHT; NONHUMAN; PARACOCCUS DENITRIFICANS; PROTEIN EXPRESSION;

3-HYDROXYBUTYRIC ACID; ACYLTRANSFERASES; ALCOHOLS; AMINO ACID SEQUENCE; BASE SEQUENCE; BIOTECHNOLOGY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE DOSAGE; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PARACOCCUS DENITRIFICANS; POLYESTERS; RECOMBINANT PROTEINS; RESTRICTION MAPPING;

EID: 0032487349     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19981005)60:1<61::AID-BIT7>3.0.CO;2-Z     Document Type: Article

References (26)

1. Anderson, A. J., Dawes, E. A. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54: 450-472.
2. Babel, W. 1992. Peculiarities of methylotrophs concerning overflow metabolism, especially the synthesis of polyhydroxyalkanoates. FEMS Microbiol. Rev. 103: 141-148.
3. Bourque, D., Ouellette, B., André, G., Groleau, D. 1992. Production of poly-β-hydroxybutyrate from methanol: characterization of a new isolate of Methylobacterium extorquens. Appl. Microbiol. Biotechnol. 37: 7-12.
4. Ditta, G., Schmidhauser, T., Yakobson, E., Peter, L., Xiao-Wu, L., Deborah, R. F., Donald, G., Helinski, D. R. 1985. Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid 13: 149-153.
5. Haywood, G. W., Anderson, A. J., Chu, L., Dawes, E. A. 1988. Characterization of two 3-ketothiolases possessing differing substrate specificities in the polyhydroxyalkanoate synthesizing organism Alcaligenes eutrophus. FEMS Microbiol. Lett. 52: 91-96.
6. Haywood, G. W., Anderson, A. J., Dawes, E. A. 1989. A survey of the accumulation of novel polyhydroxyalkanoates by bacteria. Biotechnol. Lett. 11: 471-476.
7. Hilger, U., Sattler, K., Littkowsky, U. 1991. Studies on the growth-associated accumulation of poly-β-hydroxybutyric acid with Methylobacterium rhodesianum Z. Zentraibl. Mikrobiol. 146: 83-88.
8. Lee, S. Y. 1996a. Plastic bacteria? Progress and prospects of polyhydroxyalkanoate production in bacteria. Trends Biotechnol. 14: 431-438.
9. Lee, S. Y. 1996b. Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng. 49: 1-14.
10. Laemmli, U. K. 1971. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
11. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
12. Park, J. S., Park, H. C., Huh, T. L., Lee, Y. H. 1995. Production of β-hydroxybutyrate by Alcaligenes eutrophus transformants harboring cloned phbCAB genes. Biotechnol. Lett. 17: 735-740.
13. Peoples, O. P., Sinskey, A. J. 1989. Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264: 15298-15303.
14. Sambrook, J., Fritsch, E. F., Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd edition. Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.
15. Schubert, P., Krüger, N., Steinbüchel, A. 1991. Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate)-. PHB-, biosynthetic operon: identification of the N-terminus of PHB synthase and identification of the promoter. J. Bacteriol. 173: 168-175.
16. Schubert, P., Steinbüchel, A., Schlegel, H. G. 1988. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J. Bacteriol. 170: 5837-5847.
17. Sim, S. J., Snell, K. D., Hogan, S. A., Stubbe, J., Rha, C., Sinskey, A. 1997. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nature Biotechnol. 15: 63-67.
18. Steinrücke, P., Ludwig, B. 1993. Genetics of Paracoccus denitrificans. FEMS Microbiol. Rev. 104: 83-118.
19. Ueda, S., Matsumoto, S., Takagi, A., Yamane. T. 1992. n-Amyl alcohol as a substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by bacteria. FEMS Microbiol. Lett. 98: 57-60.
20. Ueda, S., Yabutani, T., Maehara, A., Yamane, T. 1996. Molecular analysis of the poly(3-hydroxyalkanoate) synthase gene from a methylotrophic bacterium, Paracoccus denitrificans. J. Bacteriol. 178: 774-779.
21. Wieczorek, R., Steinbüchel, A., Schmidt, B. 1996. Occurrence of polyhydroxyalkanoic acid granule-associated proteins related to the Alcaligenes eutrophus H16 GA24 protein in other bacteria. FEMS Microbiol. Lett. 135: 23-30.
22. Yabutani, T., Maehara, A., Ueda, S., Yamane, T. 1995. Analysis of β-ketothiolase and acetoacetyl-CoA reductase genes of a methylotrophic bacterium, Paracoccus denitrificans, and their expression in Escherichia coli. FEMS Microbiol. Lett. 133: 85-90.
23. Yamane, T., Chen, X. F., Ueda, S. 1996a. Polyhydroxyalkanoate synthesis from alcohols during the growth of Paracoccus denitrificans. FEMS Microbiol. Lett. 135: 207-211.
24. Yamane, T., Chen, X. F., Ueda, S. 1996b. Growth-associated production of poly(3-hydroxyvalerate) from n-pentanol by a Methylotrophic bacterium, Paracoccus denitrificans. Appl. Environ. Microbiol. 62: 380-384.
25. Yamane, T., Matsuda, M., Sada, E. 1981. Application of porous Teflon tubing method to automatic fed-batch culture of microorganism. I. Mass transfer through porous Teflon tubing. Biotechnol. Bioeng. 23: 2493-2507.
26. Yamane, T., Matsuda, M., Sada, E. 1981. Application of porous Teflon tubing method to automatic fed-batch culture of microorganism. II. Automatic constant-value control of fed substrate (ethanol) concentration in semi-batch culture of yeast. Biotechnol. Bioeng. 23: 2509-2524.