A simple method for evaluating Cryptosporidium-specific antibodies used in monitoring environmental water samples

Letters in Applied Microbiology

Volumn 25, Issue 5, 1997, Pages 316-320

  Vesey, G ^a   Deere, D ^a   Weir, C J ^a   Ashbolt, N ^b   Williams, K L ^a   Veal, D A ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

FLOW CYTOMETRY; QUALITY CONTROL;

BINDING SPECIFICITIES; BIVARIATE; CRYPTOSPORIDIUM; DOTPLOT; ENVIRONMENTAL WATER SAMPLES; NON-SPECIFIC BINDING; OOCYSTS; QUANTITATIVE TOOL; SIMPLE METHOD; SIMPLE++;

ANTIBODIES;

ANTIBODY; SURFACE WATER;

ARTICLE; CRYPTOSPORIDIUM; ENVIRONMENTAL MONITORING; FLOW CYTOMETRY; IMMUNOFLUORESCENCE TEST; LIGHT SCATTERING; QUALITY CONTROL; WATER ANALYSIS;

CRYPTOSPORIDIUM;

EID: 0030698324     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1997.00237.x     Document Type: Article

References (14)

1. Anon. (1992) A survey of Cryptosporidium oocysts in surface and ground waters in the UK. By the National Cryptosporidium survey group. Journal of the Institute of Water Engineering and Management 6, 697-703.
2. ASTM (American Society for Testing and Materials) (1991) Proposed test method for Giardia cysts and Cryptosporidium oocysts in low-turbidity water by a fluorescent antibody procedure. In Annual Book of ASTM Standards 11.01. pp. 925-935. Philadelphia, PA: American Society for Testing and Materials.
3. Campbell, A.T., Robertson, L.J. and Smith, H.V. (1993) Detection of oocysts of Cryptosporidium by enhanced chemiluminescence. Journal of Microbiological Methods 17, 297-303.
4. Graczyk, T.K., Cranfield, M.R. and Fayer, R. (1996) Evaluation uf commercial enzyme immunoassay (EIA) and immunofluorescent antibody (IFA) test kits for detection of Cryptosporidium oocysts of species other than Cryptosporidium parvum. American Journal of Tropical Medicine and Hygiene 54, 274-279.
5. Haugland, R.P. (1995) Coupling of monoclonal antibodies with fluorophores. In Methods in Molecular Biology, Vol. 45: Monoclonal Antibody Protocols ed. Davis, W.C. pp. 205-221. Totowa, NJ: Davis Humana Press Inc.
6. LeChevallier, M.W., Norton, W.D. and Lee, R.G. (1991) Occurrence of Giardia spp. and Cryptosporidium spp. in surface water supplies [published erratum appears in Applied und Environmental Microbiology 1992, 58, 780], Applied and Environmental Microbiology 57, 2610-2616.
7. Lisle, J.T. and Rose, J.B. (1995) Cryptosporidium contamination of water in the USA and UK - a mini review. Aqua 44, 103-117.
8. O'Donoghue, P.J. (1995) Cryptosporidium and cryptosporidiosis in man and animals. International Journal for Parasitology 25, 1-55.
9. Rodgers, M.R., Flanigan, D.J. and Jakubowski, W. (1995) Identification of algae which interfere with the detection of Giardia cysts and Cryptosporidium oocysts and a method for alleviating this interference. Applied and Environmental Microbiology 61, 3759-3763.
10. Vesey, G., Hutton, P.E., Champion, A.C. et al. (1994a) Application of flow cytometric methods for the routine detection of Cryptosporidium and Gairdia in water. Cytometry 16, 1-6.
11. Vesey, G., Narai, J., Ashbolt, N., Williams, K.L. and Veal, D.A. (1994b) Detection of specific microorganisms in environmental samples using flow cytometry. In Methods in Cell Biology, Vol. 42: Flow Cytometry, 2nd edn ed. Darzynkiewicz, Z., Robinson, J.P. and Crissman, H.A. pp. 489-522. New York: Academic Press Inc.
12. Vesey, G., Slade, J.S., Byrne, M., Shepherd, K., Dennis, P.J. and Fricker, C.R. (1993a) Routine monitoring of Cryptosporidium oocysts in water using flow cytometry. Journal of Applied Bacteriology 75, 87-90.
13. Vesey, G., Slade, J.S., Byrne, M., Shepherd, K. and Fricker, C.R. (1993b) new method for the concentration of Cryptosporidium oocysts from water. Journal of Applied Bacteriology 75, 82-86.
14. Vesey, G., Slade, J.S. and Fricker, C.R. (1991) taking the eye strain out of environmental Cryptosporidium analysis. Letters in Applied Microbiology 13, 62-65.