Anticoagulant peptides from Ancylostoma caninum are immunologically distinct and localize to separate structures within the adult hookworm

Molecular and Biochemical Parasitology

Volumn 133, Issue 2, 2004, Pages 319-323

  Mieszczanek, Juliusz ^a,b   Harrison, Lisa M ^a   Vlasuk, George P ^c   Cappello, Michael ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WARSAW UNIVERSITY OF LIFE SCIENCES   (Poland)

^c Dendreon   (United States)

Author keywords

Ancylostoma; Coagulation; Factor VIIa; Factor Xa; Hookworm; Nematode; Tissue factor

Indexed keywords

AMINO ACID; ANTICOAGULANT AGENT; ANTICOAGULANT PEPTIDE 5; ANTICOAGULANT PROTEIN C 2; ANTICOAGULANT PROTEIN C 3; ANTICOAGULANT PROTEIN C 4; BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 7A; COMPLEMENTARY DNA; CYSTEINE; ENZYME; HISTIDINE; PEPTIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANCYLOSTOMA CANINUM; ARTICLE; BLOOD CLOTTING TIME; BODY REGIONS; CARBOXY TERMINAL SEQUENCE; CODON; CONTROLLED STUDY; DRUG ACTIVITY; DRUG TARGETING; ESCHERICHIA COLI; GENE AMPLIFICATION; GENETIC CODE; HOOKWORM; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; IN VITRO STUDY; MICROTITER PLATE ASSAY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRECURSOR; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; THROMBOPLASTIN TIME;

ANCYLOSTOMA; ANCYLOSTOMA CANINUM; ANCYLOSTOMATOIDEA; ESCHERICHIA COLI;

EID: 0348048783     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2003.10.015     Document Type: Article

References (24)

1. Albonico M., Savioli L. Hookworm infection and disease: advances for control. Ann. Ist. Super Sanita. 33:1997;567-579.
2. Crompton D.W.T. The public health importance of hookworm. J. Parasitol. 121:2000;S39-S50.
3. Kalkofen U.P. Attachment and feeding behavior of Ancylostoma caninum. Z. Parasitenkd. 33:1970;339-354.
4. Kalkofen U.P. Intestinal trauma resulting from feeding activities of Ancylostoma caninum. Am. J. Trop Med. Hyg. 23:1974;1046-1053.
5. Harrison L.M., Nerlinger A., Bungiro R.D., Cordova J.L., Kuzmic P., Cappello M. Molecular characterization of Ancylostoma inhibitors of coagulation factor Xa. Hookworm anticoagulant activity in vitro predicts parasite bloodfeeding in vivo. J. Biol. Chem. 277:2002;6223-6229.
6. Harrison L.M., Cordova J.L., Cappello M. Ancylostoma caninum anticoagulant peptide-5: immunolocalization and in vitro neutralization of a major hookworm anti-thrombotic. Mol. Biochem. Parasitol. 115:2001;101-107.
7. Chadderdon R.C., Cappello M. The hookworm platelet inhibitor: functional blockade of integrins GPIIb/IIIa (alphaIIbbeta3) and GPIa/IIa (alpha2beta1) inhibits platelet aggregation and adhesion in vitro. J. Infect Dis. 179:1999;1235-1241.
8. Stassens P., Bergum P.W., Gansemans Y.et al. Anticoagulant repertoire of the hookworm Ancylostoma caninum. Proc. Natl. Acad. Sci. U.S.A. 93:1996;2149-2154.
9. Cappello M., Hawdon J.M., Jones B.F., Kennedy W.P., Hotez P.J. Ancylostoma caninum anticoagulant peptide: cloning by PCR and expression of soluble, active protein in E. coli. Mol. Biochem. Parasitol. 80:1996;113-117.
10. Cappello M., Vlasuk G.P., Bergum P.W., Huang S., Hotez P.J. Ancylostoma caninum anticoagulant peptide: a hookworm-derived inhibitor of human coagulation factor Xa. Proc. Natl. Acad. Sci. U.S.A. 92:1995;6152-6156.
11. Cappello M., Clyne L.P., McPhedran P., Hotez P.J. Ancylostoma factor Xa inhibitor: partial purification and its identification as a major hookworm-derived anticoagulant in vitro. J. Infect Dis. 167:1993;1474-1477.
12. Del Valle A., Jones B.F., Harrison L.M., Chadderdon R.C., Cappello M. Isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult Ancylostoma caninum. Mol. Biochem. Parasitol. 129:2003;167-177.
13. Furmidge B.A., Horn L.A., Pritchard D.I. The anti-haemostatic strategies of the human hookworm Necator americanus. Parasitology. 112(Pt 1):1996;81-87.
14. Buddai S.K., Toulokhonova L., Bergum P.W., Vlasuk G.P., Krishnaswamy S. Nematode anticoagulant protein c2 reveals a site on factor Xa that is important for macromolecular substrate binding to human prothrombinase. J. Biol. Chem. 277:2002;26689-26698.
15. Bergum P.W., Cruikshank A., Maki S.L., Kelly C.R., Ruf W., Vlasuk G.P. Role of zymogen and activated factor X as scaffolds for the inhibition of the blood coagulation factor VIIa-tissue factor complex by recombinant nematode anticoagulant protein c2. J. Biol. Chem. 276:2001;10063-10071.
16. Cappello M.H.L., Bungiro R.D., Chadderdon C.R., Del Valle A., Milstone A.M., Chu D.et al. Molecular pathogenesis of hookworm anemia: prospects for a disease based vaccine. J. Parasitol. 89:2003;S158-S164.
17. Hotez P.J., Ghosh K., Hawdon J.M.et al. Experimental approaches to the development of a recombinant hookworm vaccine. Immunol. Rev. 171:1999;163-171.
18. Eiff J.A. Nature of an anticoagulant from the cephalic glands of Ancylostoma caninum. J. Parasitol. 52:1966;833-843.
19. Grasberger B.L., Clore G.M., Gronenborn A.M. High-resolution structure of Ascaris trypsin inhibitor in solution: direct evidence for a pH-induced conformational transition in the reactive site. Structure. 2:1994;669-678.
20. Huang K., Strynadka N.C., Bernard V.D., Peanasky R.J., James M.N. The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase. Structure. 2:1994;679-689.
21. Burland T.G. DNASTAR's Lasergene sequence analysis software. Methods Mol. Biol. 132:2000;71-91.
22. Donnelly K.M., Bromberg M.E., Milstone A.et al. Ancylostoma caninum anticoagulant peptide blocks metastasis in vivo and inhibits factor Xa binding to melanoma cells in vitro. Thromb. Haemost. 79:1998;1041-1047.
23. Cappello M., Bergum P.W., Vlasuk G.P., Furmidge B.A., Pritchard D.I., Aksoy S. Isolation and characterization of the tsetse thrombin inhibitor: a potent antithrombotic peptide from the saliva of Glossina morsitans morsitans. Am. J. Trop. Med. Hyg. 54:1996;475-480.
24. Cappello M., Li S., Chen X.et al. Tsetse thrombin inhibitor: bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proc. Natl. Acad. Sci. U.S.A. 95:1998;14290-14295.