Functional interaction between BMPR-II and Tctex-1, a light chain of dynein, is isoform-specific and disrupted by mutations underlying primary pulmonary hypertension

Human Molecular Genetics

Volumn 12, Issue 24, 2003, Pages 3277-3286

  Machado, Rajiv D ^a   Rudarakanchana, Nung ^b   Atkinson, Carl ^b   Flanagan, Julia A ^a   Harrison, Rachel ^a   Morrell, Nicholas W ^b   Trembath, Richard C ^a,c  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BONE MORPHOGENETIC PROTEIN RECEPTOR; CELL SURFACE RECEPTOR; DYNEIN ADENOSINE TRIPHOSPHATASE; ISOPROTEIN; LIGAND; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

ARTERY MEDIA; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CYTOPLASM; ENDOTHELIUM; EXON; GENE DISRUPTION; GENE MUTATION; HETEROZYGOSITY; HUMAN; HUMAN CELL; HUMAN TISSUE; INHERITANCE; LIGAND BINDING; LUNG ARTERY; MOLECULAR MECHANICS; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PULMONARY HYPERTENSION; SIGNAL TRANSDUCTION; SMOOTH MUSCLE; TRANSCRIPTION REGULATION;

BONE MORPHOGENETIC PROTEIN RECEPTORS, TYPE II; DYNEIN ATPASE; HELA CELLS; HUMANS; HYPERTENSION, PULMONARY; LUNG; MICROTUBULE-ASSOCIATED PROTEINS; MODELS, BIOLOGICAL; MUTATION; NUCLEAR PROTEINS; PHOSPHORYLATION; PLASMIDS; PROTEIN ISOFORMS; PROTEIN-SERINE-THREONINE KINASES; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 0348013127     PISSN: 09646906     EISSN: None     Source Type: Journal    
DOI: 10.1093/hmg/ddg365     Document Type: Article

References (33)

1. Massague, J. and Chen, Y.-G. (2000) Controlling TGF-β signalling. Genes Dev., 14, 627-644.
2. Kawabata, M., Chytil, A., and Moses, H.L. (1995) Cloning of a novel type II serine/threonine kinase receptor through interaction with the type I transforming growth factor-β receptor. J. Biol. Chem., 270, 5625-5630.
3. Liu, F., Ventura, F., Doody, J. and Massague, J. (1995) Human type II receptor for bone morphogenetic proteins (BMPs): extension of the two-kinase receptor model to the BMPs. Mol. Cell. Biol., 15, 3479-3486.
4. Iwasaki, S., Iguchi, M., Watanabe, K., Hoshino, R., Tsujimoto, M. and Kohno, M. (1999) Specific activation of the p38 mitogen-activated protein kinase signaling pathway and induction of neurite outgrowth in PC12 cells by bone morphogenetic protein-2. J. Biol. Chem., 274, 26503-26510.
5. The International PPH Consortium, Lane, K.B., Machado, R.D., Pauciulo, M.W., Thomson, J.R., Philips, J.A., Loyd, J.E., Nichols, W.C. and Trembath, R.C. (2000) Heterozygous germ-line mutations in BMPR2, encoding a TGF-β receptor, cause familial primary pulmonary hypertension. Nat. Genet., 26, 81-84.
6. Deng, Z., Morse, J.H., Slager, S.L., Cuervo, N., Moore, K.J., Venetos, G., Kalachikov, S., Cayanis, E., Fischer, S.G., Barst, R.J. et al. (2000) Familial primary pulmonary hypertension (gene PPH1) is caused by mutations in the bone morphogenetic protein receptor-II gene. Am. J. Hum. Genet., 67, 737-744.
7. Thomson, J.R., Machado, R.D., Pauciulo, M.W., Morgan, N.V, Humbert, M., Elliot, G.C., Ward, K., Yacoub, M., Mikhail, G., Rogers, P. et al. (2000) Sporadic primary pulmonary hypertension is associated with germline mutations of the gene encoding BMPR-II, a receptor member of the TGF-β family. J. Med. Genet., 37, 741-745.
8. Rubin, L.J. (1997) Primary pulmonary hypertension. New Engl. J. Med., 336, 111-117.
9. Astrom, A.K., Jin, D., Imamura, T., Roijer, E., Rosenzweig, B., Miyazono, K., ten Dijke, P. and Stenman, G. (1999) Chromosomal localization of three human genes encoding bone morphogenetic protein receptors. Mamm. Genome, 10, 299-302.
10. Machado, R.D., Pauciulo, M.W., Fretwell, N., Veal, C., Thomson, J.R., Vilarino Guell, C., Aldred, M., Brannon, C.A., Trembath, R.C. and Nichols, W.C. (2000) A physical and transcript map based upon refinement of the critical interval for PPH1 a gene for familial primary pulmonary hypertension. The International PPH Consortium. Genomics, 68, 220-228.
11. Rosenzweig, B.L., Imamura, T., Okadome, T., Cox, G.N., Yamashita, H., ten Dijke, P., Heldin, C.-H. and Miyazono, K. (1995) Cloning and characterization of a human type II receptor for bone morphogenetic proteins. Proc. Natl Acad. Sci. USA, 92, 7632-7636.
12. Nohno, T., Ishikawa, T., Saito, T., Hosokawa, K., Noji, S., Wolsing, D.H. and Rosenbaum, J.S. (1995) Identification of a human type II receptor for bone morphogenetic protein-4 that forms differential heteromeric complexes with bone morphogenetic protein type I receptors. J. Biol. Chem., 270, 22522-22526.
13. Nohe, A., Hassel, S., Ehrlich, M., Neubauer, F., Sebald, W, Henis, Y. and Knaus, P. (2001) The mode of BMP receptor oligomerization determines different BMP-2 signaling pathways. J. Biol. Chem., 277, 5330-5338.
14. Machado, R.D., Pauciulo, M.W., Thomson, J.R., Lane, K.B., Morgan. N.V., Wheeler, L., Phillips, J.A., III, Newman, J., Williams, D., Galie, N. et al. (2001) BMPR2 haploinsufficiency as the inherited molecular mechanism for primary pulmonary hypertension. Am. J. Hum. Genet., 68, 92-102.
15. Nishihara, A., Watabe, T., Imamura, T. and Miyazono, K. (2002) Functional heterogeneity of bone morphogenetic protein receptor-II mutants found in patients with primary pulmonary hypertension. Mol. Biol. Cell., 13, 3055-3063.
16. Rudarakanchana, R., Flanagan, J.A., Chen, H., Upton, P.D., Machado, R., Patel, D., Trembath, R.C. and Morrell, N.W. (2002) Functional analysis of bone morphogenetic protein type II receptor mutations underlying primary pulmonary hypertension. Hum. Mol. Genet., 11, 1517-1525.
17. Sweeney, H.L. and Holzbaur, E.L. (1996) Mutational analysis of motor proteins. Annu. Rev. Physiol., 58, 751-792.
18. 467 in the C terminus of smad 2 mediates interaction with smad 4 and is required for transforming growth factor-β signaling. J. Biol. Chem., 272, 28107-28115.
19. Atkinson, C., Stewart, S., Upton, P.D., Machado, R., Thomson, J. R., Trembath, R.C. and Morrell, N.W. (2002) Primary pulmonary hypertension is associated with reduced pulmonary vascular expression of type II bone morphogenetic protein receptor. Circulation, 105, 1672-1678.
20. DiBella, L.M., Benashski, S.E., Tedford, H.W., Harrison, A., Patel-King, R.S. and King, S.M. (2001) The tctex1/tctex2 class of dynein light chains. J. Biol. Chem., 276, 14366-14373.
21. Vaisberg, E.A., Grissom, P.M. and McIntosh, J.R. (1996) Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles. J. Cell Biol., 133, 831-842.
22. Pfister, K.K., Salate, M.W., Dillman, J.F., Vaughan, K.T., Vallee, R.B., Torre, E. and Lye, R.J. (1996) Differential expression and phosphorylation of the 74-kDa intermediate chains of cytoplasmic dynein in cultured neurons and glia. J. Biol. Chem., 271, 1687-1694.
23. Karki, S. and Holzbaur, E.L. (1995) Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex. J. Biol. Chem., 270, 28806-28811.
24. Tai, A.W., Chuang, J.-Z., Bode, C., Wolfrum, U. and Sung, C.-H. (1999) Rhodopsin's carboxy-terminal cytoplasmic tail acts as a membrane receptor for cytoplasmic dynein by binding to the dynein light chain Tctex-1. Cell, 97, 877-887.
25. Tang, Q., Staub, C.M., Gao, G., Jin, Q., Wang, Z., Ding, W., Aurigemma, R.E. and Mulder, K.M. (2002) A novel transforming growth factor-β receptor interacting protein that is also a light chain of the motor protein dynein. Mol. Cell. Biol., 13, 4484-4496.
26. Kamal, A. and Goldstein, L.S.B. (2002) Principles of cargo attachment to cytoplasmic motor proteins. Curr. Opin. Cell. Biol., 14, 63-68.
27. Tai, A.W., Chuang, J.-Z. and Sung, C.-H. (2001) Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport. J. Cell Biol., 153, 1499-1509.
28. Karki, S., Tokito, M.K. and Holzbaur, E.L.F. (1997) Casein kinase II binds to and phosphorylates cytoplasmic dynein. J. Biol. Chem., 272, 5887-5891.
29. Greenwald, J., Groppe, J., Gray, R, Wiater, E., Kwiatkowski, W, Vale, W. and Choe, S. (2003) The BMP7/ActRII extracellular domain complex provides new insights into the cooperative nature of receptor assembly. Mol. Cell, 11, 605-617.
30. Dong, C., Li, Z., Alvarez, R. Jr., Feng, X.H. and Goldschmidt-Clermont, P.J. (2000) Microtubule binding to Smads may regulate TGF beta activity. Mol. Cell, 5, 27-34.
31. James, P., Halladay, J. and Craig, E.A. (1996) Genomic libraries and a host strain designed for highly effective two-hybrid selection in yeast. Genetics, 144, 1425-1436.
32. Agatep, R., Kirkpatrick, R.D., Parchaliuk, D.L., Woods, R.A. and Gietz, R.D. (1998) Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol protocol. Tech. Tips Online, 1(51): P01525.
33. Duttweiler, H.M. (1996) A highly sensitive and non-lethal betagalactosidase plate assay for yeast. Tech. Tips Online, 1, T40040.