A Simple and Reliable Method for Preparation of Cross-Contamination-Free Plant Genomic DNA for PCR-Based Detection of Transgenes

Journal of Biochemistry and Molecular Biology

Volumn 33, Issue 6, 2000, Pages 537-540

  Hwang, Seon Kap ^a   Kim, Young Mi ^a  

^a National Institute of Animal Science   (South Korea)

Author keywords

Genetically Modified Plant; Genomic DNA; Modified CTAB; PCR; Transgene

Indexed keywords

EID: 0347899526     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Aeschbacher, R. A., Müller, J., Boller, T. and Wiemken, A. (1999) Purification of the trehalase GMTRE1 from soybean nodules and cloning of its cDNA. GMTRE1 is expressed at a low level in multiple tissues. Plant Physiol. 119, 489-495.
2. Aljanabi, S. M. and Martinez, I. (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res. 25, 4692-4693.
3. De Boer, S. H., Ward, L. J., Li, X. and Chittaranjan, S. (1995) Attenuation of PCR inhibition in the presence of plant compounds by addition of BLOTTO. Nucleic Acids Res. 23, 2567-2568.
4. Gill, K. S., Lubbers, E. L., Gill, B. S., Raupp, W. J. and Cox, T. S. (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34, 362-374.
5. Kim, C. S., Lee, C. H., Shin, J. S., Chung, Y. S. and Hyung, N. I. (1997) A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP. Nucleic Acids Res. 25, 1085-1086.
6. Klimkait, T. (2000) 'Restriction-PCR'-a superior replacement for restriction endonucleases in DNA cloning applications. J. Biochem. Mol. Biol. 33, 162-165.
7. Langridge, U., Schwall, M. and Langridge, P. (1991) Squashes of plant tissue as substrate for PCR. Nucleic Acids Res. 19, 6954.
8. Lee, K.-O., Hong, S.-H., Kim, M.-J., Park, T.-K., Kim, Y.-J. and Lee, K.-P. (1997) Molecular analysis of HLA-C using polymerase chain reaction-sequence specific primers. J. Biochem. Mol. Biol. 30, 26-32.
9. Lin, J. J., Fleming, R., Kuo, J., Matthews, B. S. and Saunders, J. A. (2000) Detection of plant genes using a rapid, nonorganic DNA purification method. BioTechniques 28, 346-350.
10. Lipp, M., Brodmann, P., Pietsch, K., Pauwels, J., Anklam, E., Borchers, T., Braunschweiger, G., Busch, U., Eklund, E., Eriksen, F. D., Fagan, J., Fellinger, A., Gaugitsch, H., Hayes, D., Hertel, C., Hortner, H., Joudrier, P., Kruse, L., Meyer, R., Miraglia, M., Muller, W., Phillipp, P., Popping, B., Rentsch, R., Wurtz, A. (1999) IUPAC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder. J. AOAC. Int. 82, 923-928.
11. Rogers, S. G. (1998) Biotechnology and the soybean. Am. J. Clin. Nutr. 68 (suppl.), 1330S-1332S.
12. Steiner, J. J., Poklemba, C. J., Fjellstrom, R. G. and Elliott, L. F. (1995) A rapid one-tube genomic DNA extraction process for PCR and RAPD analyses. Nucleic Acids Res. 23, 2569-2570.
13. Stewart, C. N. Jr. and Via, L. E. (1993) A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques 14, 748-750.