'Restriction-PCR' - A Superior Replacement for Restriction Endonucleases in DNA Cloning Applications

Journal of Biochemistry and Molecular Biology

Volumn 33, Issue 2, 2000, Pages 162-165

  Klimkait, Thomas ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Cloning; Ligase free; Partial digest; PCR; Restriction endonuclease

Indexed keywords

EID: 0034422801     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Ailenberg, M. and Silverman, M. (1996) Description of a one step staggered reannealing method for directional cloning of PCR-generated DNA using sticky-end ligation without employing restriction enzymes. Biochem. Mol. Biol. Int. 39, 771-779.
2. Clark, J.M. (1988) Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 16, 9677-9686.
3. Liu, Z. (1996) Hetero-stagger cloning: efficient and rapid cloning of PCR products. Nucleic Acids Res. 24, 2458-2459.
4. Mezei, L.M. and Storts, D.R. (1994) In: PCR technology: Current Innovations, Griffin H.G. and Griffin, A.M., (Eds.), CRC Press, Boca Raton, FL, 21
5. Newton, C.R. and Graham, A. (1994) In PCR. BIOS Scientific Publishers, Ltd., Oxford, UK, 13