Quantum Dots as a Novel Immunofluorescent Detection System for Cryptosporidium parvum and Giardia lamblia

Applied and Environmental Microbiology

Volumn 70, Issue 1, 2004, Pages 597-598

  Zhu, Liang ^a   Ang, Simon ^b   Liu, Wen Tso ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b University of Arkansas   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYES; FLUORESCENCE; MICROORGANISMS; SIGNAL TO NOISE RATIO;

PHOTOSTABILITY;

SEMICONDUCTOR QUANTUM DOTS;

DYE;

ARTICLE; AUTOFLUORESCENCE; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; FLUORESCENCE; GIARDIA LAMBLIA; IMMUNOFLUORESCENCE; MOLECULAR STABILITY; NONHUMAN; QUANTUM MECHANICS; SEMICONDUCTOR; SIGNAL NOISE RATIO;

ANIMALS; ANTIBODIES, PROTOZOAN; CRYPTOSPORIDIUM PARVUM; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; GIARDIA LAMBLIA; QUANTUM DOTS;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; GIARDIA; GIARDIA INTESTINALIS; PROTOZOA;

EID: 0347634258     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.1.597-598.2004     Document Type: Article

References (9)

1. Bruchez, M., Jr., M. Moronne, P. Gin, S. Weiss, and A. P. Alivisatos. 1998. Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013-2016.
2. Chan, W. C., and S. Nie. 1998. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016-2018.
3. Egyed, Z., T. Sreter, Z. Szell, and I. Varga. 2003. Characterization of Cryptosporidium spp. - recent developments and future needs. Vet. Parasitol. 111:103-114.
4. Fayer, R., U. Morgan, and S. J. Upton. 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol. 30:1305-1322.
5. Goldman, E. R., E. D. Balighian, H. Mattoussi, M. K. Kuno, J. M. Mauro, P. T. Tran, and G. P. Anderson. 2002. Avidin: a natural bridge for quantum dot-antibody conjugates. J. Am. Chem. Soc. 124:6378-0382.
6. Jaiswal, J. K., H. Mattoussi, J. M. Mauro, and S. M. Simon. 2003. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 21:47-51.
7. Quintero-Betancourt, W., E. R. Peele, and J. B. Rose. 2002. Cryptosporidium parvum and Cyclospora cayetanensis: a review of laboratory methods for detection of these waterborne parasites. J. Microbiol. Methods 49:209-224.
8. Vesey, G., D. Deere, M. R. Gauci, K. R. Griffiths, K. L. Williams, and D. A. Veal. 1997. Evaluation of fluorochromes and excitation sources for immunofluorescence in water samples. Cytometry 29:147-154.
9. Wu, X., H. Liu, J. Liu, N. Haley Kari, J. A. Treadway, J. P. Larson, N. Ge, F. Peale, and M. P. Bruchez. 2003. Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat. Biotechnol. 21:41-46.