DNA-based subtyping of verocytotoxin-producing Escherichia coli (VTEC) O128ab:H2 strains from human and raw meat sources

Letters in Applied Microbiology

Volumn 37, Issue 6, 2003, Pages 433-437

  Domingue, G ^a,b   Willshaw, G A ^a   Smith, H R ^a   Perry, N ^a   Radford, D ^a   Cheasty, T ^a  

^a PUBLIC HEALTH ENGLAND   (United Kingdom)

^b UNIVERSITY OF EXETER   (United Kingdom)

Author keywords

Long PCR; Pulsed field gel electrophoresis; Subtyping; Verocytotoxin producing Escherichia coli O128ab:H2

Indexed keywords

ELECTROPHORESIS; FORMAL LANGUAGES; GENES; POLYMERASE CHAIN REACTION; STRAIN;

ENCODINGS; INTIMIN; LONG-PCR; PLASMID PROFILE; PULSED FIELD GEL ELECTROPHORESIS; RANDOMLY AMPLIFIED POLYMORPHIC DNA; RAW MEATS; SUBTYPINGS; VEROCYTOTOXIN; VEROCYTOTOXIN- PRODUCING ESCHERICHIA COLI O128AB:H2;

ESCHERICHIA COLI;

GENOMIC DNA; VEROTOXIN;

BACTERIUM;

ARTICLE; BACTERIUM ISOLATE; DNA SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI O157; FOOD CONTAMINATION; MEAT; NONHUMAN; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; RANDOM AMPLIFIED POLYMORPHIC DNA; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0347517760     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.2003.01424.x     Document Type: Article

References (25)

1. Barrett, T.J., Lior, H., Green, J.H., Khakhria, R., Wells, J.G., Bell, B.P., Greene, K.D., Lewis, J. et al. (1994) Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing. Journal of Clinical Microbiology 32, 3013-3017.
2. Bastian, S.N., Carle, I. and Grimont, F. (1998) Comparison of 14 PCR systems for the detection and subtyping of stx genes in Shiga-producing Escherichia coli. Research Microbiology 149, 457-472.
3. Bettelheim, K.A. (2000) Role of non-O157 VTEC. Journal of Applied Bacteriology Symposium Supplement 88, 38S-50S.
4. Beutin, L., Geier, D., Zimmermann, Z., Aleksic, S., Gillespie, H.A. and Whittam, T.S. (1997) Epidemiological relatedness and clonal types of natural populations of Escherichia coli strains producing Shiga toxins in separate populations of cattle and sheep. Applied and Environmental Microbiology 63, 2175-2180.
5. Birch, M., Denning, D.W. and Law, D. (1996) Rapid genotyping of Escherichia coli O157 isolates by random amplification of polymorphic DNA. European Journal of Clinical Microbiology and Infectious Diseases 15, 297-302.
6. Birnboim, H.C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant DNA. Nucleic Acid Research 1, 1513-1523.
7. 1c is the most common Shiga toxin 1 subtype among Shiga toxin-producing Escherichia coli isolates from sheep but not among isolates from cattle. Journal of Clinical Microbiology 41, 926-936.
8. Datz, M., Janetzki-Mittman, C, Franke, S., Gunzer, F., Schmidt, H. and Karch, H. (1996) Analysis of the enterohemorrhagic Escherichia coli O157 DNA region containing lamboid phage gene p and Shiga-like toxin structural genes. Applied and Environmental Microbiology 62, 791-797.
9. Goldwater, P.N. and Bettelheim, K.A. (1998) New perspectives on the role of Escherichia coli O157:H7 and other enterohaemorraghic Escherichia coli serotypes in human disease. Journal of Medical Microbiology 47, 1039-1045.
10. Heuvelink, A.E., van de Kar, N.C.A.J., Meis, J.F.G.M., Monnens, L.A.H. and Melchers, W.J.G. (1995) Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uraemic syndrome in Western Europe. Epidemiology and Infection 115, 1-14.
11. Kaper, J.B. and O'Brien, A.D. (1998) Escherichia coli O157:H7 and Other Shiga Toxin-Producing E. coli Strains. Washington, DC: American Society for Microbiology Press.
12. Karch, H., Bielaszewska, M., Bitzan, M. and Schmidt, H. (1999) Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. Diagnostic Microbiology and Infectious Disease 34, 229-243.
13. Law, D. (2000) Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli. Journal of Applied Microbiology 88, 729-745.
14. Lin, Z., Kurazono, H., Yamasaki, S. and Takeda, Y. (1993) Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiology and Immunology 37, 543-548.
15. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
16. Pierard, D., Muyldermans, G., Moriau, L., Stevens, D. and Lauwers, S. (1998) Identification of new verocytoxin type 2 variant B-subunit genes in human and animal isolates. Journal of Clinical Microbiology 36, 3317-3322.
17. Schmidt, H., Geitz, C., Tarr, P.I., Frosch, M. and Karch, H. (1999) Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality. Journal of Infectious Diseases 179, 115-123.
18. Stephan, R., Ragettli, S. and Untermann, F. (2000) Prevalence and characteristics of verotoxin-producing Escherichia coli (VTEC) in stool samples from asymptomatic human carriers working in the meat processing industry in Switzerland. Journal of Applied Microbiology 88, 335-341.
19. Thomas, A., Smith, H.R. and Rowe, B. (1993) Use of digoxigenin-labelled oligonucleotide DNA probes for VT2 and VT2 human variant genes to differentiate vero cytotoxin-producing Escherichia coli strains of serogroup O157. Journal of Clinical Microbiology 31, 1700-1703.
20. Watanabe, H., Wada, A., Inagaki, Y., Itoh, K. and Tamura, K. (1996) Outbreaks of enterohaemorrhagic Escherichia coli O157:H7 infection by two different genotype strains in Japan. Lancet 348, 831-832.
21. Willshaw, G.A., Scotland, S.M., Smith, H.R. and Rowe, B. (1992) Properties of vero cytotoxin-producing Escherichia coli of human origin of O serogroups other than 0157. Journal of Infectious Diseases 166, 797-802.
22. Willshaw, G.A., Smith, H.R., Roberts, D., Thirlwell, J., Cheasty, T. and Rowe, B. (1993) Examination of raw beef products for the presence of verocytotoxin producing Escherichia coli, particularly those of sero-group O157. Journal of Applied Bacteriology 75, 420-426.
23. Willshaw, G.A., Smith, H.R., Cheasty, T., Wall, P.G. and Rowe, B. (1997) Vero cytotoxin-producing Escherichia coli O157 outbreaks in England and Wales, 1995: phenotypic methods and genotypic subtyping. Emerging Infectious Diseases 3, 561-565.
24. Willshaw, G.A., Cheasty, T., Smith, H.R., O'Brien, S.J. and Adak, G.K. (2001) Vero cytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995 to 1998. Journal of Medical Microbiology 50, 135-142.
25. 1c) in Escherichia coli strains isolated from humans. Journal of Clinical Microbiology 40, 1441-1446.