Jellyfish green fluorescent protein as a useful reporter for transient expression and stable transformation in Medicago sativa L.

Plant Cell Reports

Volumn 22, Issue 5, 2003, Pages 328-337

  Bellucci, M ^a   De Marchis, F ^a   Mannucci, R ^b   Arcioni, S ^a  

^a CNR   (Italy)

^b UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Bombardment; Green fluorescent protein; Plastid targeting; Transgenic alfalfa; Transit peptide

Indexed keywords

CROPS; FLUORESCENCE; GENES; PROTEINS;

CHLOROPLASTS;

PLANTS (BOTANY);

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; VEGETABLE PROTEIN; ZEIN;

ALFALFA; ARTICLE; CHLOROPLAST; CONFOCAL MICROSCOPY; CYTOLOGY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MUTATION; TRANSGENIC PLANT;

CHLOROPLASTS; GENE EXPRESSION REGULATION, PLANT; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MEDICAGO SATIVA; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MUTATION; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT FUSION PROTEINS; ZEIN;

MEDICAGO; MEDICAGO SATIVA; SCYPHOZOA; ZEA MAYS;

EID: 0347415867     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-003-0693-7     Document Type: Article

References (36)

1. Bagga S, Adams HP, Rodriguez FD, Kemp JD, Sengupta-Gopalan C (1997) Coexpression of the maize δ-zein and β-zein genes results in stable accumulation of δ-zein in endoplasmic reticulum-derived protein bodies formed by β-zein. Plant Cell 9:1683-1696
2. Bellucci M, Arcioni S (2002) Correct balance of the protein content in food and fodder crops through genetic engineering. In: Pandalai SG (ed) Recent research developments in plant biology. Research Signpost 2:137-146
3. Bellucci M, Alpini A, Arcioni S (2002) Coexpression of γ-zein:KDEL and β-zein:KDEL polypeptides in tobacco leaf and zein accumulation in forage species (Lotus corniculatus and Medicago sativa). Plant Cell Rep 20:848-856
4. Brown DCW, Atanassov A (1985) Role of genetic background in somatic embryogenesis in Medicago. Plant Cell Tissue Organ Cult 4:111-122
5. Davis SJ, Vierstra RD (1998) Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol 36:521-528
6. Elliott AR, Campbell JA, Dugdale B, Brettel RIS, Grof CPL (1999) Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells. Plant Cell Rep 18:707-714
7. Ferro M, Salvi D, Riviere-Rolland H, Vermat T, Seigneurin-Berny D, Grunwald D, Garin J, Joyard J, Rolland R (2002) Integral membrane proteins of the chloroplast envelope: identification and subcellular localization of new transporters. Proc Natl Acad Sci USA 99:11487-11492
8. Geest AHM van der, Petolino JF (1998) Expression of a modified green fluorescent protein gene in transgenic maize plants and progeny. Plant Cell Rep 17:760-764
9. Guerineau F, Woolston S, Brooks L, Mullineaux P (1988) An expression cassette for targeting foreign proteins into chloroplasts. Nucleic Acids Res 16:11380
10. Harper BK, Mabon SA, Leffel SM, Halfhill MD, Richards HA, Moyer KA, Stewart CN Jr (1999) Green fluorescent protein as a marker for expression of a second gene in transgenic plants. Nat Biotechnol 17:1125-1129
11. Haseloff J, Amos B (1995) GFP in plants. Trends Genet 11:328-329
12. Haseloff J, Siemering KR (1998) The uses of GFP in plants. In: Chalfie M, Kain SR (eds) Green fluorescent protein: properties, applications, and protocols. Wiley, Chichester, pp 191-220
13. Haseloff J, Siemering KR, Prasher DC, Hodge S (1997) Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc Natl Acad Sci USA 94:2122-2127
14. Heim R, Prasher DC, Tsien RY (1994) Wavelength mutation and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA 91:12501-12504
15. Heim R, Cubitt AB, Tsien RY (1995) Improved green fluorescence. Nature 373:663-664
16. Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42:819-832
17. Hibbered JM, Linley PJ, Khan MS, Gray JC (1998) Transient expression of green fluorescent protein in various plastid types following microprojectile bombardment. Plant J 16:627-632.
18. Horton RM, Hunt HD, Ho SN, Pullen JK, Pease LR (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68
19. Jang IC, Nahm BH, Kim JK (1999) Subcellular targeting of green fluorescent protein to plastids in transgenic rice plants provides a high-level expression system. Mol Breed 5:453-461
20. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901-3908
21. Kim CS, Woo Y, Clore A, Burnett RJ, Carneiro NP, Larkins BA (2002) Zein protein interactions, rather than the asymmetric distribution of zein mRNAs on endoplasmic reticulum membranes, influence protein body formation in maize endosperm. Plant Cell 14:655-672
22. Köhler RH, Cao J, Zipfel WR, Webb WW, Hanson MR (1997) Exchange of protein molecules through connection between higher plant plastids. Science 276:2039-2042
23. Leffel SM, Mabon SA, Stewart CN Jr (1997) Green fluorescent protein as a marker for monitoring transgenic plants. In: McLean GD, Waterhouse PM, Evans G, Gibbs MJ (eds) Commercialisation of transgenic crops: risk, benefit and trade considerations. CRC for Plant Science and Bureau of Resources Sciences, Canberra, pp 193-199
24. Mercuri A, Sacchetti A, De Benedetti L, Schiva T, Alberti S (2001) Green fluorescent flowers. Plant Sci 161:961-968
25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497
26. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85-87
27. Pang SZ, DeBoer DL, Wan Y, Ye G, Layton JG, Neher MK, Armstrong CL, Fry JE, Hinchee MAW, Fromm M (1996) An improved green fluorescent protein gene as a vital marker in plants. Plant Physiol 112:893-900
28. Pereira LF, Erickson L (1995) Stable transformation of alfalfa (Medicago sativa L.) by particle bombardment. Plant Cell Rep 14:290-293
29. Ponappa T, Brzozowski AE, Finer JJ (1999) Transient expression and stable transformation of soybean using the jellyfish green fluorescent protein. Plant Cell Rep 19:6-12
30. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229-233
31. Reichel C, Mathur J, Eckes P, Langenkemper K, Koncz C, Schell J, Reiss B, Maas C (1996) Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc Natl Acad Sci USA 93:5888-5893
32. Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW (1995) Green Fluorescent Protein as a new vital marker in plant cells. Plant J 8:777-784
33. Siemering KR, Golbik R, Sever R, Haseloff J (1996) Mutations that suppress the thermosensitivity of green fluorescent protein. Curr Biol 6:1653-1663
34. Stewart CN Jr (2001) The utility of green fluorescent protein in transgenic plants. Plant Cell Rep 20:376-382
35. Vain P, Worland B, Kohli A, Snape J, Christou P (1998) The green fluorescent protein as a vital screenable marker in rice transformation. Theor Appl Genet 96:164-169
36. Wang S, Hazelrigg T (1994) Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature 369:400-403