Purification of Electron-Transferring Flavoprotein from Megasphaera elsdenii and Binding of Additional FAD with an Unusual Absorption Spectrum

Journal of Biochemistry

Volumn 134, Issue 5, 2003, Pages 719-729

  Sato, Kyosuke ^a   Nishina, Yasuzo ^a   Shiga, Kiyoshi ^a  

^a KUMAMOTO UNIVERSITY   (Japan)

Author keywords

Electron transferring flavoprotein; FAD binding; FAD release; Megasphaera elsdenii; Unusual absorption spectrum

Indexed keywords

BUTYRYL CYCLIC AMP; FLAVINE ADENINE NUCLEOTIDE; FLAVOPROTEIN; GUANIDINE; LACTATE DEHYDROGENASE; OXIDOREDUCTASE;

ABSORPTION SPECTROPHOTOMETRY; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; CHEMICAL MODIFICATION; CRYSTALLOGRAPHY; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME SUBUNIT; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN PURIFICATION;

ACIDAMINOCOCCACEAE; AMINO ACID SEQUENCE; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTRON-TRANSFERRING FLAVOPROTEINS; FLAVIN-ADENINE DINUCLEOTIDE; LASERS; LIGHT; MOLECULAR WEIGHT; OXIDATION-REDUCTION; SPECTROPHOTOMETRY, INFRARED; SPECTROPHOTOMETRY, ULTRAVIOLET;

ANIMALIA; BACTERIA (MICROORGANISMS); MEGASPHAERA ELSDENII; SUS SCROFA;

EID: 0347363568     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg199     Document Type: Article

References (39)

1. Thorpe, C. (1991) Electron-transferring flavoprotein in Chemistry and Biochemistry of Flavoenzymes (Müller, F., ed.) Vol. 2, pp. 471-486, CRC Press, Boca Raton, Ann Arbor, Boston, London
2. Gorelick, R.J., Mizzer, J.P., and Thorpe, C. (1982) Purification and properties of electron-transferring flavoprotein from pig kidney. Biochemistry 21, 6936-6942
3. Sato, K., Nishina, Y., and Shiga, K. (1993) Electron-transferring flavoprotein has an AMP-binding site in addition to the FAD-binding site. J. Biochem. 114, 215-222
4. Steenkamp, D.J. and Gallup, M. (1978) The natural flavoprotein electron acceptor of trimethylamine dehydrogenase. J. Biol. Chem. 253, 4086-4089
5. DuPlessis, E.R., Rohlfs, R.J., Hille, R., and Thorpe, C. (1994) Electron-transferring flavoprotein from pig and the methylotrophic bacterium W3A1 contains AMP as well as FAD. Biochem. Mol. Biol. Int. 32, 195-199
6. Husain, M. and Steenkamp, D.J. (1985) Partial purification and characterization of glutaryl-coenzyme A dehydrogenase, electron transfer flavoprotein, and electron transfer flavoprotein-Q oxidoreductase from Paracoccus denitrificans. J. Bacteriol. 163, 709-715
7. Griffin, K.J., Dwyer, T.M., Manning, M.C., Meyer, J.D., Carpenter, J.F., and Frerman, F.E. (1997) αT244M Mutation affects the redox, kinetic, and in vitro folding properties of Paracoccus denitrificans electron transfer flavoprotein. Biochemistry 36, 4194-4202
8. Whitfield, C.D. and Mayhew, S.G. (1974) Purification and properties of electron-transferring flavoprotein from Peptostreptococcus elsdenii. J. Biol. Chem. 249, 2801-2810
9. Brockman, H.L. and Wood, W.A. (1975) Electron-transferring flavoprotein of Peptostreptococcus elsdenii that functions in the reduction of acrylyl-coenzyme A. J. Bacteriol. 124, 1447-1453
10. Hetzel, M., Brock, M., Selmer, T., Pierik, A.J., Golding, B.T., and Buckel, W. (2003) Acryloyl-CoA reductase from Clostridium propionicum: An enzyme complex of propionyl-CoA dehydrogenase and electron-transferring flavoprotein. Eur. J. Biochem. 270, 902-910
11. Roberts, D.L., Frerman, F.E., and Kim, J.-J.P (1996) Three-dimensional structure of human electron transfer flavoprotein to 2.1-Å resolution. Proc. Natl. Acad. Sci. USA 93, 14355-14360
12. Roberts, D.L., Salazar, D., Fulmer, J.P., Frerman, F.E., and Kim, J.-J.P. (1999) Crystal structural of Paracoccus denitrificans electron transfer flavoprotein: structural and electrostatic analysis of a conserved flavin binding domain. Biochemistry 38, 1977-1989
13. O'Neill, H., Mayhew, S.G., and Butler, G. (1998) Cloning and analysis of the genes for a novel electron-transferring flavoprotein from Megasphaera elsdenii. J. Biol. Chem. 273, 21015-21024
14. Sato, K., Nishina, Y., and Shiga, K. (1997) In vitro assembly of FAD, AMP, and the two subunits of electron-transferring flavoprotein: an important role of AMP related with the conformational change of the apoprotein. J. Biochem. 121, 477-486
15. Mayhew, S.G. and Ludwig, M.L. (1975) Flavodoxins and electron-transferring flavoproteins Enzymes 12, 57-118
16. Lehman, T.C. and Thorpe, C. (1990) Alternate electron acceptors for medium-chain acyl-CoA dehydrogenase: Use of ferricenium salts. Biochemistry 29, 10594-10602
17. Sato, K., Nishina, Y., Setoyama, C., Miura, R., and Shiga, K. (1999) Unusually high standard redox potential of acrylyl-CoA/propionyl-CoA couple among enoyl-CoA/acyl-CoA couples: A reason for the distinct metabolic pathway of propionyl-CoA from longer acyl-CoAs. J. Biochem. 126, 668-675
18. Laemmli, U.K. (1970) Cleavage of structural proteins during the assemble of the head of bacteriophage T4. Nature 227, 680-685
19. Hayashi, Y., Matsui, H., and Takagi, T. (1989) Membrane protein molecular weight determined by low-angle laser light-scattering photometry coupled with high-performance gel chromatography. Methods Enzymol. 172, 514-528
20. Takagi, T. (1990) Application of low-angle laser light scattering detection in the field of biochemistry: Review of recent progress. J. Chromatogr. 506, 409-416
21. Gill, S.C. and von Hippel, P.H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319-326
22. Hendler, R.W. and Shrager, R.I. (1994) Deconvolutions based on singular value decomposition and the pseudoinverse: a guide for beginners. J. Biochem. Biophys. Method. 28, 1-33
23. Stadtman, E.R (1957) Preparation and assay of acyl coenzyme A and other thiol esters; Use of hydroxylamine. Methods Enzymol. 3, 931-941
24. Engel, P.C. (1981) Butyryl-CoA dehydrogenase from Megasphaera elsdenii. Methods Enzymol. 71, 359-366
25. Olson, S.T. and Massey, V. (1979) Purification and properties of the flavoenzyme D-lactate dehydrogenase from Megasphaera elsdenii. Biochemistry 18, 4714-4724
26. Geoghegan, S.M., Mayhew, S.G., Yalloway, G.N., and Butler G. (2000) Cloning, sequencing and expression of the gene for flavodoxin from Megasphaera elsdenii and the effects of removing the protein negative charge that is closest to N(1) of the bound FMN. Eur. J. Biochem. 267, 4434-4444
27. Becker, D.F., Fuchs, J.A., Banfield, D.K., Funk, W.D., MacGillivray, R.T.A., and Stankovich, M.T. (1993) Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii. Biochemistry 32, 10736-10742
28. Engel, P.C. and Massey, V. (1971) The purification and properties of butyryl-coenzyme A dehydrogenase from Peptostreptococcus elsdenii. Biochem. J. 125, 879-887
29. Williamson, G., Engel, P.C., Mizzer, J.P., Thorpe, C., and Massey, V. (1982) Evidence that the greening ligand in native butyryl-CoA dehydrogenase is a CoA persulfide. J. Biol. Chem. 257, 4314-4320
30. Williamson, G. and Engel, P.C. (1982) A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase. Biochim. Biophys. Acta 706, 245-248
31. Tanaka, M., Haniu, M., Yasunobu, K.T., Mayhew, S., and Massey, V. (1973) The primary structure of Peptostreptococcus elsdenii flavodoxin. J. Biol. Chem. 248, 4354-4366
32. Tanaka, M., Haniu, M., Yasunobu, K.T., Mayhew, S., and Massey, V. (1974) Correction of the amino acid sequence of Peptostreptococcus elsdenii flavodoxin. J. Biol. Chem. 249, 4397-4397
33. Mayhew, S.G. and Massey, V. (1969) Purification and characterization of flavodoxin from Peptostreptococcus elsdenii. J. Biol. Chem. 244, 794-802
34. Sato, K., Nishina, Y., and Shiga, K. (1992) Anion-induced conformational change of apo-electron-transferring flavoprotein. J. Biochem. 111, 359-365
35. Dwyer, T.M., Mortl, S., Kemter, K., Bacher, A., Fauq, A., and Frerman, F.E. (1999) The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer. Biochemistry 38, 9735-9745
36. Pace, C.P. and Stankovich, M.T. (1987) Redox properties of electron-transferring flavoprotein from Megasphaera elsdenii. Biochim. Biophys. Acta 911, 267-276
37. Sato, K., Nishina, Y., and Shiga, K. (2002) Reduction of electron-transferring flavoprotein from Megasphaera elsdenii by NADH and D-lactate dehydrogenase in Flavins and Flavoproteins 2002 (Chapman, S., Perham, R., and Scrutton, N., eds.) pp. 641-645, Rudolf Weber, Agency for Scientific Publications, Berlin
38. Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J.-P., Huber, R., and Bacher, A. (2002) Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe. Eur. J. Biochem. 269, 519-526
39. Whitfield, C.D. and Mayhew, S.G. (1974) Evidence that apo-reduced nicotinamide adenine dinucleotide dehydrogenase and apo-electron-transferring flavoprotein from Peptostreptococcus elsdenii are identical. J. Biol. Chem. 249, 2811-2815