Characterization and performance evaluation of in vivo and in vitro produced monoclonal anti-TNT antibodies for the detection of TNT

Journal of Immunological Methods

Volumn 284, Issue 1-2, 2004, Pages 15-26

  Charles, Paul T ^a   Shriver Lake, Lisa C ^a   Francesconi, Stephen C ^b   Churilla, Albert M ^a   Rangasammy, Jermain G ^a   Patterson Jr , Charles H ^a   Deschamps, Jeffrey R ^a   Kusterbeck, Anne W ^a  

^a NAVAL RESEARCH LABORATORY   (United States)

^b NOVA RESEARCH INC   (United States)

Author keywords

Antibody; Explosives; Fluorescence; Immunosensor; Microcapillary; TNT

Indexed keywords

AROMATIC NITRO COMPOUND; FLUORESCENT DYE; METHYL 2,4,6 TRINITROTOLUENE; MONOCLONAL ANTIBODY; TRINITROBENZENE; TRINITROTOLUENE ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY DETECTION; ARTICLE; BINDING AFFINITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENTAL TEST; FLUORESCENCE; IC 50; IMMUNOASSAY; IMMUNOSENSOR; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0347003699     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2003.09.010     Document Type: Article

References (27)

1. Altstein M., Bronshtein A., Glattstein B., Zeichner A., Tamiri T., Almog J. Immunochemical approaches for purification and detection of TNT traces by antibodies entrapped in a sol-gel matrix. Anal. Chem. 73:2001;2461-2467.
2. Bakaltcheva I.B., Ligler F.S., Patterson C.H. Jr., Shriver-Lake L.C. Multi-analyte explosive detection using a fiber optic biosensor. Anal. Chim. Acta. 399:1999;13-20.
3. Buttner W.J., Findlay M., Vickers W., Davis W.M., Cespedes E.R., Cooper S., Adams J.W. In-situ detection of trinitrotoluene and other nitrated explosives in soil. Anal. Chim. Acta. 341:1997;63-71.
4. Cell Essentials, www.cell-essentials.com/custom-monoclonal-frameset.htm.
5. Charles P.T., Kusterbeck A.W. Trace level detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by microimmunosensor. Biosens. Bioelectron. 14:1999;387-396.
6. Charles P.T., Gauger P.R., Patterson C.H. Jr., Kusterbeck A.W. On-site immunoanalysis of nitrate and nitroaromatic compounds in groundwater. Environ. Sci. Technol. 34:2000;4641-4650.
7. Crockett A.B., Craig H.D., Jenkins T.F., Sisk W.E. Field sampling and selecting on-site analytical methods for explosives in soil. Federal Facilities Forum Issue; EPA/540/R-97/50. 1996;1-32 U.S. Government Printing Office, Washington, DC.
8. de Boer M., Admiraal P., Kok K., Ossendorp F.A., de Vijlder J.J., Tager J.M. Characterization of five monoclonal antibodies obtained after immunization in vitro with a synthetic 19-amino acid peptide of thyroglobulin. Hybridoma. 6(6):1987;655-662.
9. Gauger P.R., Holt D.B., Patterson C.H. Jr., Charles P.T., Shriver-Lake L., Kusterbeck A.W. Explosives detection in soil using a field-portable continuous flow immunosensor. J. Hazard. Mater. 83:2001;51-63.
10. Goode A., Wolfsy L., Henry C., Kimura J. Mishell B.B., Shiigi S.M. Selected Methods in Cellular Immunology. 1980;345 Freeman, New York.
11. Greene T.M., Charles P.T., Anderson G.P. Detection of 2,4,6-trinitrotoluene in seawater using a reversed-displacement immunosensor. Anal. Biochem. 310:2002;36-41.
12. Heiss C., Weller M.G., Niessner R. Dip-and-read test strips for the determination of trinitrotoluene (TNT) in drinking water. Anal. Chim. Acta. 396:1999;309-316.
13. Helmerson K., Kishore R., Phillips W.D., Weetall H.H. An optical tweezers-based immunosensor for detection of femtomoles-per-liter concentrations of antigens. Appl. Biochem. Biotechnol. 96:2001;205-213.
14. Holt D.B., Gauger P.R., Kusterbeck A.W., Ligler F.S. Fabrication of capillary immunosensor in polymethyl methacrylate. Biosens. Bioelectron. 17:2002;95-103.
15. Kusterbeck A.W., Charles P.T. Field demonstration of a portable flow immunosensor. Field Anal. Chem. Technol. 2:1998;341-350.
16. Kusterbeck A.W., Charles P., Patterson C., Gauger P. Displacement immunoassay for TNT and RDX using continuous flow immunosensor. Lopez-Avila V., Barcelo D., Beckert W., Goheen S.T., Jinno K., Keith L.H., Rittenburg J.H. Current Protocol in Field Analytical Chemistry. Suppl. 4:2000;2C.11.1-2C.11.14 John Wiley & Sons, Inc. New York, NY.
17. Naal Z., Park J.H., Bernhard S., Shapleigh J., Batt C.A., Abruna H.D. Amperometric TNT biosensor based on the oriented immobilization of a nitroreductase maltose binding protein fusion. Anal. Chem. 74:2002;140-148.
18. Narang U., Gauger P.R., Ligler F.S. A displacement flow immunosensor for explosive detection using microcapillaries. Anal. Chem. 69:1997;2779-2785.
19. Narang U., Gauger P.R., Kusterbeck A.W., Ligler F.S. Multi-analyte detection using a capillary-based flow immunosensor. Anal. Biochem. 255:1998;13-19.
20. Rabbany S.Y., Kusterbeck A.W., Bredehorst R., Ligler F.S. Binding kinetics of immobilized antibodies in flow immunosensor. Sens. Actuators, B. 29:1995;72-78.
21. Rabbany S.Y., Marganski W.A., Kusterbeck A.W., Ligler F.S. A membrane-based displacement flow immunoassay. Biosens. Bioelectron. 13:1998;939-944.
22. Rabbany S.Y., Piervincenzi R.T., Kusterbeck A.W., Bredehorst R., Ligler F.S. Dissociation rate kinetics in a solid-phase flow immunoassay. Anal. Lett. 31:1998;1663-1675.
23. Sapsford K.E., Charles P.T., Patterson C.H. Jr., Ligler F.S. Demonstration of four immunoassay formats using the array biosensor. Anal. Chem. 74:2002;1061-1068.
24. Shriver-Lake L.C., Breslin K.A., Charles P.T., Conrad D.W., Golden J.P., Ligler F.S. Detection of TNT in water using an evanescent wave fiber-optic biosensor. Anal. Chem. 67:1995;2431-2435.
25. Van Bergen S.K., Bakaltcheva I.B., Lundgren J.S., Shriver-Lake L.C. On-site detection of explosives in groundwater with a fiber optic biosensor. Environ. Sci. Technol. 34:2000;704-708.
26. Yinon J., Zitrin S. Modern Methods and Application in Analysis of Explosives. 1993;Wiley, New York, NY.
27. Zeck A., Weller M., Niessner R. Characterization of a monoclonal anti-TNT antibody by measurement of the cross-reactivities of nitroaromatic compounds. Fresenius' J. Anal. Chem. 364:1999;113-120.