Protein kinase A regulates AKAP250 (gravin) scaffold binding to the β2-adrenergic receptor

EMBO Journal

Volumn 22, Issue 24, 2003, Pages 6419-6429

  Tao, Jiangchuan ^a   Wang, Hsien Yu ^a   Malbon, Craig C ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

adrenergic receptor; AKAP; Gravin; Protein kinase A; Protein kinase C; Scaffold

Indexed keywords

A KINASE ANCHORING PROTEIN 250; BETA 2 ADRENERGIC RECEPTOR; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; PHOSPHOPROTEIN PHOSPHATASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME REGULATION; HUMAN; HUMAN CELL; METABOLISM; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; RECEPTOR BINDING;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CARCINOMA, SQUAMOUS CELL; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DNA PRIMERS; GREEN FLUORESCENT PROTEINS; HUMANS; KINETICS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN KINASE C; PROTEINS; RECEPTORS, ADRENERGIC, BETA-2; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; TUMOR CELLS, CULTURED;

EID: 0346993722     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdg628     Document Type: Article

References (19)

1. Alto, N., Carlisle Michel, J.J., Dodge, K.L., Langeberg, L.K. and Scott, J.D. (2002) Intracellular targeting of protein kinases and phosphatases. Diabetes, 51, Suppl. 8.
2. Bauman, A.L. and Scott, J.D. (2002) Kinase- and phosphatase-anchoring proteins: harnessing the dynamic duo. Nature Cell Biol., 4, 203-206.
3. Colledge, M. and Scott, J.D. (1999) AKAPs: from structure to function. Trends Cell Biol., 9, 216-221.
4. Cong, M., Perry, S.J., Lin, F.T., Fraser, I.D., Hu, L.A., Chen, W., Pitcher, J.A., Scott, J.D. and Lefkowitz, R.J. (2001) Regulation of membrane targeting of the G protein-coupled receptor kinase 2 by protein kinase A and its anchoring protein AKAP79. J. Biol. Chem., 276, 15192-15199.
5. Dell'Acqua, M.L., Dodge, K.L., Tavalin, S.J. and Scott, J.D. (2002) Mapping the protein phosphatase-2B anchoring site on AKAP79. Binding and inhibition of phosphatase activity are mediated by residues 315-360. J. Biol. Chem., 277, 48796-48802.
6. Fan, G., Shumay, E., Malbon, C.C. and Wang, H. (2001a) c-Src tyrosine kinase binds the β2-adrenergic receptor via phospho-Tyr-350, phosphorylates G-protein-linked receptor kinase 2 and mediates agonist-induced receptor desensitization. J. Biol. Chem., 276, 13240-13247.
7. Fan, G., Shumay, E., Wang, H.H. and Malbon, C.C. (2001b) The scaffold protein gravin (AKAP250) binds the β2-adrenergic receptor via the receptor cytoplasmic R329 to L413 domain and provides a mobile scaffold during desensitization. J. Biol. Chem., 276, 24005-24014.
8. Gelman, I.H. (2002) The role of SSeCKS/gravin/AKAP12 scaffolding proteins in the spaciotemporal control of signaling pathways in oncogenesis and development. Front. Biosci., 7, 1782-1797.
9. Hausken, Z.E. and Scott, J.D. (1996) Properties of A-kinase anchoring proteins. Biochem. Soc. Trans, 24, 986-991.
10. Karoor, V. and Malbon, C.C. (1996) Insulin-like growth factor receptor-1 stimulates phosphorylation of the β2-adrenergic receptor in vivo on sites distinct from those phosphorylated in response to insulin. J. Biol. Chem., 271, 29347-29352.
11. Kim, S.H., Lee, S.K. and Choi, K.Y. (1998) Saccharomyces cerevisiae STE11 may contribute to the stabilities of a scaffold protein, STE5, in the pheromone signaling pathway. Mol. Cell, 8, 130-137.
12. Lin, F., Wang, H. and Malbon, C.C. (2000) Gravin-mediated formation of signaling complexes in β2-adrenergic receptor desensitization and resensitization. J. Biol. Chem., 275, 19025-19034.
13. Michel, J.J. and Scott, J.D. (2002) AKAP mediated signal transduction. Annu. Rev. Pharmacol. Toxicol., 42, 235-257.
14. Morris, A.J. and Malbon, C.C. (1999) Physiological regulation of G protein-linked signaling. Physiol. Rev., 79, 1373-1430.
15. Nauert, J.B., Klauck, T.M., Langeberg, L.K. and Scott, J.D. (1997) Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Curr. Biol., 7, 52-62.
16. Oliveria, S.F., Gomez, L.L. and Dell'Acqua, M.L. (2003) Imaging kinase-AKAP79-phosphatase scaffold complexes at the plasma membrane in living cells using FRET microscopy. J. Cell Biol., 160, 101-112.
17. Pawson, T. and Scott, J.D. (1997) Signaling through scaffold, anchoring and adaptor proteins. Science, 278, 2075-2080.
18. Shih, M. and Malbon, C.C. (1994) Oligodeoxynucleotides antisense to mRNA encoding protein kinase A, protein kinase C and β-adrenergic receptor kinase reveal distinctive cell-type-specific roles in agonist-induced desensitization. Proc. Natl Acad. Sci. USA, 91, 12193-12197.
19. Shih, M., Lin, F., Scott, J.D., Wang, H.Y. and Malbon, C.C. (1999) Dynamic complexes of β2-adrenergic receptors with protein kinases and phosphatases and the role of gravin. J. Biol. Chem., 274, 1588-1595.