Solvent and Primary Deuterium Isotope Effects Show That Lactate CH and OH Bond Cleavages Are Concerted in Y254F Flavocytochrome b2, Consistent with a Hydride Transfer Mechanism

Biochemistry

Volumn 42, Issue 51, 2003, Pages 15208-15214

  Sobrado, Pablo ^a,b   Fitzpatrick, Paul F ^a,b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b UNIVERSITY OF CHILE   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

AROMATIC COMPOUNDS; CATALYSIS; CHEMICAL BONDS; DEUTERIUM; ENZYMES; OXIDATION; SOLVENTS;

TRANSFER MECHANISM;

YEAST;

CARBON; DEUTERIUM; FLAVOPROTEIN; HYDROGEN; HYDROXYACID; ISOTOPE; LACTATE DEHYDROGENASE (CYTOCHROME); LACTIC ACID; OXYGEN; PHENYLALANINE; SOLVENT; TYROSINE;

ARTICLE; CATALYSIS; CHEMICAL BINDING; CHEMICAL BOND; CHEMICAL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME STRUCTURE; MEASUREMENT; NONHUMAN; OXIDATION; PH; PRIORITY JOURNAL; PROTEIN FAMILY; WILD TYPE;

CATALYSIS; DEUTERIUM; DEUTERIUM EXCHANGE MEASUREMENT; HYDROGEN; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; HYDROXY ACIDS; KINETICS; L-LACTATE DEHYDROGENASE (CYTOCHROME); LACTIC ACID; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; SACCHAROMYCES CEREVISIAE PROTEINS; SOLVENTS; TYROSINE;

EID: 0346963173     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035546n     Document Type: Article

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