The catalytic role of tyrosine 254 in flavocytochrome b2 (L-lactate dehydrogenase from baker's yeast): Comparison between the Y254F and Y254L mutant proteins

European Journal of Biochemistry

Volumn 268, Issue 18, 2001, Pages 4918-4927

  Gondry, Muriel ^a,b   Dubois, Joëlle ^a,c   Terrier, Micheline ^a   Lederer, Florence ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

^b CEA SACLAY   (France)

^c CNRS   (France)

Author keywords

Enzyme mechanism; Flavocytochrome b2; Hydrogen bond; L hydroxy acid oxidation; Site directed mutagenesis

Indexed keywords

ALANINE; ATROLACTIC ACID; ENZYME INHIBITOR; FLAVOPROTEIN; HISTONE; HYDROGEN; HYDROXY ACID OXIDASE A; ION; LACTATE DEHYDROGENASE; LACTATE DEHYDROGENASE (CYTOCHROME); LACTIC ACID; LEUCINE; MANDELIC ACID; MUTANT PROTEIN; PYRUVIC ACID; TYROSINE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CATALYSIS; CATALYST; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; MOLECULAR MODEL; MUTATION; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SACCHAROMYCES CEREVISIAE;

AMINO ACID SUBSTITUTION; BINDING SITES; CATALYSIS; ENZYME INHIBITORS; HYDROGEN BONDING; KINETICS; L-LACTATE DEHYDROGENASE; L-LACTATE DEHYDROGENASE (CYTOCHROME); LACTATES; LACTIC ACID; LIGANDS; MANDELIC ACIDS; MUTATION; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; TYROSINE;

ANIMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0034799618     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02424.x     Document Type: Article

References (59)

1. Utilization by yeast of D-lactate and L-lactate as sources of energy in the presence of antimycin
2. 2
3. 2 at 2.4 Å resolution
4. Amino acid sequence of long chain α-hydroxy-acid oxidase from rat kidney, a member of the family of FMN-dependent α-hydroxy-acid-oxidizing enzymes
5. L-lactate oxidase and L-lactate monooxygenase: Mechanistic variations on a common structural theme
6. Refined structure of spinach glycolate oxidase at 2 Å resolution
7. 2 are structurally homologous and evolutionarily related enzymes with distinctly different function and flavin mononucleotide binding
8. L-lactate oxidase
9. Dehydrogenation mechanism in flavoprotein catalysis
10. 2
11. 2 (baker's yeast). Deuterium isotope effect studied by rapid-kinetic methods as a probe for the mechanism of electron transfer
12. 2 from baker's yeast
13. The chemical mechanism of flavoprotein-catalysed α-hydroxy-acid dehydrogenation: A mutational analysis
14. 2: Consequences on catalysis of lactate dehydrogenation
15. Weakly polar interactions in proteins
16. Role of tyrosine 129 in the active site of spinach glycolate oxidase
17. Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase
18. 2
19. 2 are expressed in E coli as a mixture of holoenzyme and flavin-free protein
20. 2 (yeast L-lactate dehydrogenase)
21. (S) -Mandelate dehydrogenase from Pseudomonas putida: Mechanistic studies with alternate substrates and pH and kinetic isotope effects
22. 2 mutants forms Y254L and D282N
23. 2 in Escherichia coli
24. Determination of enzyme inhibition constants
25. A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors
26. 2)
27. 2
28. 2. Primary kinetic isotope effect studies with a phenylalanine mutant
29. 2 (yeast L-lactate dehydrogenase)
30. Calculation of substrate dissociation constants from steady-state isotope effects in enzymecatalyzed reactions
31. Steady-state analysis of kinetic isotope effects in enzymic reactions
32. 2); the flavodehydrogenase function
33. 2
34. 2: Role of the driving force in intramolecular electron transfer kinetics
35. 2) from Saccharomyces cerevisiae
36. 2: Introduction of L-mandelate dehydrogenase activity
37. The reactivity of flavoproteins with sulfite. Possible relevance to the problem of oxygen reactivity
38. 2
39. 2-sulfite complex
40. 2 from baker's yeast
41. Différence entre les propriétés de la L-lactico-déshydrogénase physiologique et la L-lacticodéshydrogénase cristallisée de la levure
42. 2 (L-(+) lactate dehydrogenase)
43. 2
44. 2-by product binding to the semiquinone transient: Loss of reactivity towards monoelectronic acceptors
45. 2 (L-lactate dehydrogenase): Substitution of Asp282 with asparagine
46. Studies on the mechanism of action of the flavoenzyme lactate oxidase. Proton uptake and release during the binding of transition state analogs
47. Lactate monooxygenase. III. Additive contributions of active site residues to catalytic efficiency and stabilization of an anionic transition state analogue
48. 2: A study with wild-type and Y143F mutant enzymes
49. 2
50. 2 mutant crystallized in the presence of lactate or phenyllactate
51. The hydrogen bond in molecular recognition
52. Basis of biological specificity
53. Differential binding energy: A detailed evaluation of the influence of hydrogen bonding and hydrophobic groups on the inhibition of thermolysin by phosphorous-containing inhibitors
54. 2
55. Studies on the mechanism of action of D-amino acid oxidase. Evidence for removal of substrate α-hydrogen as a proton
56. Studies on the mechanism of action of the flavoenzyme lactate oxidase. Oxidation and elimination with β-chlorolactate
57. 2
58. Three-dimensional structure of porcine kidney D-amino acid oxidase at 3.0 Å resolution
59. TURBO-FRODO