Deletion analysis identifies key functional domains of the cytadherence-associated protein HMW2 of Mycoplasma pneumoniae

Molecular Microbiology

Volumn 50, Issue 5, 2003, Pages 1507-1516

  Balish, Mitchell F ^a   Ross, Stephanie M ^a   Fisseha, Makda ^a,b   Krause, Duncan C ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b WORLD HEALTH ORGANIZATION   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION PROTEIN P1; BACTERIAL PROTEIN; CYTOADHERENCE ASSOCIATED PROTEIN; HMW1 PROTEIN; HMW2 PROTEIN; HMW3 PROTEIN; P65 PROTEIN; PROTEIN P28; TRITON X 100; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIUM ADHERENCE; BACTERIUM MUTANT; BIOGENESIS; C TERMINAL DOMAIN; CELL ADHESION; CELL ORGANELLE; CELL STRUCTURE; CELLULAR DISTRIBUTION; CLUSTER ANALYSIS; CYTOSKELETON; DELETION ANALYSIS; DELETION MUTANT; DNA VECTOR; FRAMESHIFT MUTATION; HMW2 GENE; MUTATIONAL ANALYSIS; MYCOPLASMA PNEUMONIAE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN STABILITY; RECOMBINANT GENE; STRUCTURE ACTIVITY RELATION; TRANSLATION INITIATION; VIRUS CELL TRANSFORMATION;

ADHESINS, BACTERIAL; BACTERIAL ADHESION; BACTERIAL PROTEINS; ESCHERICHIA COLI; GENE DELETION; MICROSCOPY, ELECTRON, SCANNING; MYCOPLASMA PNEUMONIAE; ORGANELLES; RECOMBINATION, GENETIC; STRUCTURE-ACTIVITY RELATIONSHIP; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); MYCOPLASMA; MYCOPLASMA PNEUMONIAE; RICKETTSIALES;

EID: 0346887117     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2003.03807.x     Document Type: Article

References (36)

1. Balish, M.F., Hahn, T.-W., Popham, P.L., and Krause, D.C. (2001) Stability of Mycoplasma pneumonias cytadherence-accessory protein HMW1 correlates with its association with the triton shell. J Bacteriol 183: 3680-3688.
2. Balish, M.F., Santurri, R.T., Ricci, A., Lee, K.K., and Krause, D.C. (2003) Localization of Mycoplasma pneumoniae cytadherence-associated protein HMW2 by fusion with green fluorescent protein: implications for attachment organelle structure. Mol Microbiol 47: 49-60.
3. Baseman, J.B., Cole, R.M., Krause, D.C., and Leith, D.K. (1982) Molecular basis for cytadsorption of Mycoplasma pneumoniae. J Bacteriol 151: 1514-1522.
4. Cheng, S., Fockler, C., Barnes, W.M., and Higuchi, R. (1994) Effective amplification of long targets from cloned inserts and human genomic DNA. Proc Natl Acad Sci USA 91: 5695-5699.
5. Dallo, S.F., Kannan, T.R., Blaylock, M.W., and Baseman, J.B. (2002) Elongation factor Tu and E1 beta subunit of pyruvate dehydrogenase complex act as fibronectin binding proteins in Mycoplasma pneumoniae. Mol Microbiol 46: 1041-1051.
6. Feldner, J., Göbel, U., and Bredt, W. (1982) Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature (London) 298: 765-767.
7. Fisseha, M., Göhlmann, H.W.H., Herrmann, R., and Krause, D.C. (1999) Identification and complementation of frame-shift mutations associated with loss of cytadherence in Mycoplasma pneumoniae. J Bacteriol 181: 4404-4410.
8. Göbel, U., Speth, V., and Bredt, W. (1981) Filamentous structures in adherent Mycoplasma pneumoniae cells treated with nonionic detergents. J Cell Biol 91: 537-543.
9. Hahn, T.-W., Willby, M.J., and Krause, D.C. (1998) HMW1 is required for cytadhesin P1 trafficking to the attachment organelle in Mycoplasma pneumoniae. J Bacteriol 180: 1270-1276.
10. Hayflick, L. (1965) Tissue cultures and mycoplasmas. Tex Rep Biol Med 23 (Suppl. 1): 285-303.
11. Hedreyda, C.T., and Krause, D.C. (1995) Identification of a possible cytadherence regulatory locus in Mycoplasma pneumoniae. Infect Immun 63: 3479-3483.
12. Hedreyda, C.T., Lee, K.K., and Krause, D.C. (1993) Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation. Plasmid 30: 170-175.
13. Hilbert, H., Himmelreich, R., Plagens, H., and Herrmann, R. (1996) Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comprising the dnaA region, the atp operon and a cluster of ribosomal protein genes. Nucleic Acids Res 24: 628-639.
14. Himmelreich, R., Hilbert, H., Plagens, H., Pirkl, E., Li, B.-C., and Herrmann, R. (1996) Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae. Nucleic Acids Res 24: 4420-4449.
15. Hu, P.-C., Cole. R.M., Huang, Y.S., Graham, J.A., Gardner, D.E., Collier, A.M., and Clyde, W.A., Jr (1982) Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science 216: 313-316.
16. Jordan, J.L., Berry, K.M., Balish, M.F., and Krause, D.C. (2001) Stability and subcellular localization of cytadherence-associated protein P65 in Mycoplasma pneumoniae. J Bacteriol 183: 7387-7391.
17. Knudtson, K.L., and Minion, F.C. (1993) Construction of Tn4001lac derivatives to be used as promoter probe vectors in mycoplasmas. Gene 137: 217-222.
18. Krause, D.C. (1998) Mycoplasma pneumoniae cytadherence: organization and assembly of the attachment organelle. Trends Microbiol 6: 15-18.
19. Krause, D.C., and Balish, M.F. (2001) Structure, function, and assembly of the terminal organelle of Mycoplasma pneumoniae. FEMS Microbiol Lett 198: 1-7.
20. Krause, D.C., Leith, D.K., Wilson, R.M., and Baseman, J.B. (1982) Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect Immun 35: 809-817.
21. Krause, D.C., Proft, T., Hedreyda, C.T., Hilbert, H., Plagens, H., and Herrmann, R. (1997) Transposon mutagenesis reinforces the correlation between Mycoplasma pneumoniae cytoskeletal protein HMW2 and cytadherence. J Bacteriol 179: 2668-2677.
22. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.
23. Layh-Schmitt, G. (1993) The ORF6 gene product of the P1 operon of Mycoplasma pneumoniae. Zentralbl Bakteriol 278: 287-295.
24. Layh-Schmitt, G., and Herrmann, R. (1994) Spatial arrangement of gene products of the P1 operon in the membrane of Mycoplasma pneumoniae. Infect Immun 62: 974-979.
25. Lipman, R.P., and Clyde, W.A., Jr (1969) The interrelationship of virulence, cytadsorption and peroxide formation in Mycoplasma pneumoniae. Proc Soc Exp Biol Med 131: 1163-1167.
26. Meng, K.E., and Pfister, R.M. (1980) Intracellular structures of Mycoplasma pneumoniae revealed after membrane removal. J Bacteriol 144: 390-399.
27. Morrison-Plummer, J., Leith, D.K., and Baseman, J.B. (1986) Biological effects of anti-lipid and anti-protein monoclonal antibodies on Mycoplasma pneumoniae. Infect Immun 53: 398-403.
28. Popham, P.L., Hahn, T.-W., Krebes, K.A., and Krause, D.C. (1997) Loss of HMW1 and HMW3 in noncytadhering mutants of Mycoplasma pneumoniae occurs post-translationally. Proc Natl Acad Sci USA 94: 13979-13984.
29. Proft, T., and Herrmann, R. (1994) Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins. Mol Microbiol 13: 337-348.
30. Romero-Arroyo, C.E., Jordan, J., Peacock, S.J., Willby, M.J., Farmer, M.A., and Krause, D.C. (1999) Mycoplasma pneumonlae protein P30 is required for cytadherence and associated with proper cell development. J Bacteriol 181: 1079-1087.
31. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Plainview, NY: Cold Spring Harbor Laboratory Press.
32. Seto, S., and Miyata, M. (2003) Attachment organelle formation represented by localization of cytadherence proteins and formation of the electron-dense core in wild-type and mutant strains of Mycoplasma pneumoniae. J Bacteriol 185: 1082-1091.
33. Stevens, M.K., and Krause, D.C. (1991) Localization of the Mycoplasma pneumoniae cytadherence-accessory proteins HMW1 and HMW4 in the cytoskeletonlike Triton shell. J Bacteriol 173: 1041-1050.
34. Stevens, M.K., and Krause, D.C. (1992) Mycoplasma pneumoniae cytadherence phase-variable protein HMW3 is a component of the attachment organelle. J Bacteriol 174: 4265-4274.
35. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
36. Willby, M.J., and Krause, D.C. (2002) Characterization of a Mycoplasma pneumoniae hmw3 mutant: implications for attachment organelle assembly. J Bacteriol 184: 3061-3068.