Heterologous overexpression and purification of four common subunits of nuclear RNA polymerases I, II and III of Schizosaccharomyces pombe

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 800, Issue 1-2, 2004, Pages 121-126

  Proshkin, Sergey A ^a   Shpakovski, George V ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

Cloning; Expression; Nuclear RNA polymerases; Purification

Indexed keywords

AFFINITY CHROMATOGRAPHY; ELECTROPHORESIS; ENZYME KINETICS; ESCHERICHIA COLI; FILTRATION; GEL PERMEATION CHROMATOGRAPHY; GENETIC ENGINEERING; MASS SPECTROMETRY; POLYAMIDES; PROTEINS;

GEL ELECTROPHORESIS; GENE EXPRESSION; GENE TRANSCRIPTION;

RNA;

DNA DIRECTED RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE III; FUNGAL PROTEIN; HYBRID PROTEIN; PROTEIN RPB10; PROTEIN RPB5; PROTEIN RPB8; PROTEIN RPC10; PROTEIN SUBUNIT; RECOMBINANT PROTEIN; RNA POLYMERASE II; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; CONFERENCE PAPER; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL FILTRATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY;

ESCHERICHIA COLI; EUKARYOTA; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE; VECTORS;

EID: 0346786414     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2003.09.008     Document Type: Conference Paper

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