Optimal Preparation of Rhesus Macaque Blood for Cytokine Flow Cytometric Analysis

Cytometry Part A

Volumn 53, Issue 1, 2003, Pages 28-38

  Nomura, Laurel E ^a   DeHaro, Eileen D ^b   Martin, Louis N ^b   Maecker, Holden T ^a  

^a BD BIOSCIENCES   (United States)

^b TULANE NATIONAL PRIMATE RESEARCH CENTER   (United States)

Author keywords

Cellular immune response; Flow cytometry; Intracellular cytokines; Rhesus macaque; Simian immunodeficiency virus; T lymphocytes

Indexed keywords

CD4 ANTIGEN; CD69 ANTIGEN; CD8 ANTIGEN; CYTOKINE; FLUOROCHROME; GAG PROTEIN; MONOCLONAL ANTIBODY; STAPHYLOCOCCUS ENTEROTOXIN B; SUPERANTIGEN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; BLOOD SMEAR; CELL COUNT; CELL SEPARATION; CELLULAR IMMUNITY; CONTROLLED STUDY; CYTOKINE FLOW CYTOMETRY; DENSITY GRADIENT; ERYTHROCYTE PRESERVATION; FEMALE; FICOLL DENSITY GRADIENT SEPARATION; FLOW CYTOMETRY; FLUORESCENCE; FREEZING; HUMAN IMMUNODEFICIENCY VIRUS; IMMUNE RESPONSE; LABORATORY DIAGNOSIS; LYMPHOCYTE COUNT; MALE; MONONUCLEAR CELL; NONHUMAN; PRIORITY JOURNAL; RHESUS MONKEY; SIMIAN IMMUNODEFICIENCY VIRUS; STAINING; T LYMPHOCYTE SUBPOPULATION; TEST TUBE; TUBE;

EID: 0346754919     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10038     Document Type: Article

References (31)

1. Desrosiers RC. The simian immunodeficiency viruses. Annu Rev Immunol 1990;8:557-578.
2. Baskin GB, Murphey-Corb M, Watson E, Martin LN. Necropsy findings in rhesus monkeys experimentally infected with cultured simian immunodeficiency virus (SIV)/Delta. Vet Pathol 1988;25:456-467.
3. Letvin NL, Schmitz JE, Jordan HL, Seth A, Hirsch VM, Reimann KA, Kuroda MJ. Cytotoxic T lymphocytes specific for the simian immunodeficiency virus. Immunol Rev 1999;70:127-134.
4. Lori F, Lewis MG, Xu J, Varga G, Zinn DE Jr., Crabbs C, Wagner W, Greenhouse J, Silvera P, Yalley-Ogunro J, Tinelli C, Lisziewicz J. Control of SIV rebound through structured treatment interruptions during early infection. Science 2000;290:1591-1593.
5. Gauduin M-C, Glickman RL, Ahmad S, Yilma T, Johnson RP. Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and β-chemokine production. Proc Natl Acad Sci USA 1999;96:14031-14036.
6. + lymphocytes in control of simian immunodeficiency virus infection and resistance to rechallenge after transient early antiretroviral treatment. J Virol 2001;75:10187-10199.
7. Miller MD, Lord CI, Stallard V, Mazzara GP, Letvin NL. Gag-specific cytotoxic T lymphocytes in rhesus monkeys infected with the simian immunodeficiency virus of macaques. J Immunol 1990;144:122-128.
8. Kaur A, Grant RM, Means RE, McClure H, Feinberg M, Johnson RP. Diverse host responses and outcomes following simian immunodeficiency viras SIVmac239 infection in sooty mangabeys and rhesus monkeys. J Virol 1998;72:9597-9611.
9. + T-lymphocyte proliferation and activation in acute simian immunodeficiency virus infection. J Virol 2000;74:8413-8424.
10. Amara RR, Villinger F, Altman JD, Lydy SL, O'Neil SP, Staprans SI, Montefiori DC, Xu Y, Herndon JG, Wyatt LS, Candido MA, Kozyr NL, Earl PL, Smith JM, Ma H-L, Grimm BD, Hulsey ML, Miller J, McClure HM, McNicholl JM, Moss B, Robinson HL. Control of a mucosal challenge and prevention of AIDS by a multiprotein DMA/MVA vaccine. Science 2001;292:69-74.
11. Hel A, Nacsa J, Kelsall B, Tsai W-P, Letvin N, Parks RW, Tryniszewska E, Picker L, Lewis MG, Edgehill-Smith Y, Moniuszko M, Pal R, Stevceva L, Altman JD, Allen TM, Watkins D, Torres JV, Berzofsky JV, Belyakov IM, Strober W, Franchini G. Impairment of gag-specific CD8+ T-cell function in mucosal and systemic compartments of simian immunodeficiency virus mac251- and simian-human immunodeficiency virus KU2-infected macaques. J Virol 2001;75:11483-11495.
12. Schmitz JE, Kuroda MJ, Veazy RS, Seth A, Taylor WM, Nickerson CE, Lifton MA, Dailey PJ, Forman MA, Racz P, Tenner-Racz K, Letvin NL. Simian immunodeficiency virus (SIV)-specific CTL are present in large numbers in livers of SIV-infected rhesus monkeys. J Immunol 2000;164:6015-6019.
13. Schmitz JE, Veazey RS, Kuroda MJ, Levy DB, Seth A, Mansfield KG, Nickerson, CE, Lifton MA, Alvarez X, Lackner AA, Letvin NL. Simian immunodeficiency virus (SIV)-specific cytotoxic T lymphocytes in gastrointestinal tissues of chronically SIV-infected rhesus monkeys. Blood 2001;98:3757-3761.
14. Barouch DH, Santra S, Schmitz JE, Kuroda MJ, Fu T-M, Wagner W, Bilska M, Craiu A, Zheng XX, Krivulka GR, Beaudry K, Lifton MA, Nickerson CE, Trigona WL, Punt K, Freed DC, Guan L, Dubey S, Casimiro D, Simon A, Davies M-E, Chastain M, Strom TB, Gelman RS, Montefiori DC, Lewis MG, Emini EA, Shiver JW, Letvin NL. Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science 2000;290:486-492.
15. + T lymphocytes in naïve and simian immunodeficiency virus-infected rhesus monkeys. AIDS Res Hum Retroviruses 2001;17:873-886.
16. Pitcher CJ, Hagen SI, Walker JM, Lum R, Mitchell BL, Maino VC, Axthelm MK, Picker LJ. Development and homeostasis of T cell memory in rhesus macaque. J Immunol 2002;168:29-43.
17. Maecker HT, Maino VC, Picker LJ. Immunofluorescence analysis of T-cell responses in health and disease. J Clin Immunol 2000;20:391-399.
18. Murphey-Corb M, Martin LN, Rangan SRS, Baskin BB, Gormus BJ, Wolf RH, Andes WA, West M, Montelaro RC. Isolation of an HTLV-III-related retrovirus from macaques with simian AIDS and its possible origin in asymptomatic mangabeys. Nature 1986;321:435-437.
19. Amedee AM, Lacour N, Gierman JL, Martin LN, Clements JE, Bohm R Jr, Harrison RB, Murphey-Corb M. Genotypic selection of simian immunodeficiency virus in macaque infants infected transplacentally. J Virol 1995;69:7982-7990.
20. Miller CJ, Marthas M, Torten J, Alexander NJ, Moore JP, Doncel GF, Hendrickx AG. Intravaginal inoculation of rhesus macaques with cell-free simian immunodeficiency virus results in persistent or transient viremia. J Virol 1994;68:6391-6400.
21. Trivedi P, Horejsh D, Hinds SB, Hinds PW II, Wu MS, Salvato MS, Pauza CD. Intrarectal transmission of simian immunodeficiency virus in rhesus macaques: selective amplification and host responses to transient or persistent viremia. J Virol 1996;70:6876-6883.
22. Martin LN, Murphey-Corb M, Mack P, Baskin GB, Pantaleo G, Vacarezza M, Fox CH, Fauci AS. Cyclosporin A modulation of early virologic and immunologic events during primary simian immunodeficiency virus infection. J Infect Dis 1997;176:374-383.
23. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J Immunol Methods 1998;212:89-98.
24. + T cells. Cytometry 2000;40:60-68.
25. Waldrop SL, Davis KA, Maino VC, Picker LJ. Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cy-tokine synthesis. J Immunol 1998;161:5284-5295.
26. Openshaw P, Murphy EE, Hosken NA, Maino V, Davis K, Murphy K, O'Garra A. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med 1995;182:1357-1367.
27. Chardin P, McCormick F. Brefeldin A: the advantage of being uncompetitive. Cell 1999;97:153-155.
28. Capitanio JP, Mendoza SP, McChesney M. Influences of blood sampling procedures on basal hypothalamic-pituitary-adrenal hormone levels and leukocyte values in rhesus macaques (Macaca mulatta). J Med Primatol 1996;25:26-33.
29. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation. Cytometry 1995;20:127-133.
30. Cole SC, Rowles JL, Jagerski BA, Murphey-Corb M, Unangst T, Clements JE, Robinson J, Wyand MS, Desrosiers RC, Montelaro RC. Evolution of envelope-specific antibody responses in monkeys experimentally infected or immunized with simian immunodeficiency virus and its association with the development of protective immunity. J Virol 1997;71:5069-5079.
31. + T lymphocyte turnover in simian immunodeficiency virus-infected macaques. Proc Natl Acad Sci USA 1998;95:6388-6393.