Detection of non-host viable contaminants in Pichia pastoris cultures and fermentation broths

Journal of Industrial Microbiology and Biotechnology

Volumn 30, Issue 11, 2003, Pages 643-650

  Plantz, Bradley A ^a   Andersen, Jackie ^a   Smith, Leonard A ^b   Meagher, Michael M ^a   Schlegel, Vicki L ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^b UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

Author keywords

Fermentation; Non host contaminants; Pichia pastoris

Indexed keywords

RECOMBINANT PROTEIN;

ARTICLE; ASSAY; BACTERIUM; FERMENTATION; FUNGAL CONTAMINATION; FUNGAL DETECTION; FUNGAL STRAIN; FUNGUS CULTURE; GENETICS; GROWTH, DEVELOPMENT AND AGING; HOST; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; NONHUMAN; PHENOTYPE; PICHIA; PICHIA PASTORIS; PROTEIN EXPRESSION; YEAST;

BACTERIOLOGICAL TECHNIQUES; FERMENTATION; INDUSTRIAL MICROBIOLOGY; PHENOTYPE; PICHIA; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); FUNGI; PICHIA; PICHIA PASTORIS;

EID: 0346688595     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10295-003-0089-2     Document Type: Article

References (20)

1. Bar KA, Hopkins SA, Sreekrshna K (1992) Protocol for efficient secretion of HAS developed from P. pastoris. Pharm Eng 12:48-51
2. Barnett JA, Payne PW, Yarrow D (1979) In: Barnett JA, Payne RW, Yorrow D (eds) A guide to identifying and classifying yeasts. Cambridge University Press, Cambridge
3. Center for Biologics Evaluation and Research (1993) Points to consider in the characterization of cell lines used to produce biologicals. Food and Drug Administration, Rockville, Maryland, USA
4. Code of Federal Regulations, General biological product standards: sterility, Title 21 Part 610.12. US Government Printing Office, Washington, DC,http://www.fda.gov/cdrh/ devadvice/part610.html
5. Sneath PHA, Mair, NS, Sharpe ME (1984) In: Holt G (ed) Bergey's manual of systematic bacteriology, vol 2. Williams and Wilkins, Baltimore, pp 1016-1017, 1122-1123
6. Cregg JM, Tschopp JF, Stillman C, Siegel R, Akong M, Craig WS, Buckholz RG, Maden KR, Kellais PA, Davis GR, Smiley BL, Cruze J, Rorregrossa R, Velicelibi G, Thill GP (1987) High Expression and efficient assembly of hepatitis B surface antigen in methylotrophic yeast, P. Pastoris. Bio/Technol 5:479-485
7. Diggon ME, Tschoop JF, Grinna L, Lair SV, Craig WS, Velicelebi GR, Thill GP (1988) Secretion of heterologous proteins from methylotrophic yeast, P. pastoris. Dev Ind Microbiol 29:59-65
8. Higgins DR, Cregg JM (1998) Introduction to Pichia Pastoris. In: Higgins DR, Cregg JM (eds) Pichia protocols. Humana, Totowa, New Jersey, pp 1-15
9. International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (1997) Q5D: Derivation and characterization of cell substrates used for production of biotechnological/ biological broduct. Geneva, Switzerland
10. Larouch Y, Strme V, DeMuetter J, Messens J, Lauwerys M (1994) High level secretion and very efficient isotopic labeling of tich anticoagulent peptide (TAP) expressed in the methylotrophic yeast, P. pastoris. Bio/Technol 12:1119-1124
11. Lodder J, (ed) (1970) In: The yeasts. North Holland, Amsterdam
12. Peng YC, Acheson NH (1997) Production of active polyomavirus large T antigen in yeast, P. pastoris. Virus Res 49:41-47
13. Siegel RS, Brierley RA (1989) Methylotrophic yeast P. pastoris produced in high cell density fermentations with high cell yields as a vehicle for recombinant protein production. Biotechnol Bioeng 34:403-404
14. Smith PM, Suphioglu D, Griffith IJ, Theriault K, Know RB, Singh MB (1996) Cloning and expression in yeast, P. pastoris, of a biologically active form of Cynd1, the major allergen of Bermuda grass pollen. J Allergy Clin Immunol 93:331-343
15. Stratton J, Chinuvolu V, Meagher M (1998) High cell density fermentation. In: Higgins DR, Cregg JM (eds) Pichia protocols. Humana, Totowa, New Jersey, pp. 95-106
16. Tscopp JF, Sverlow G, Kosson, Craig RW, Ginna L (1989) High level secretion of glycosylated invertase in methylotrophic yeast, P. pastoris. Bio/Technol. 5:1305-1308
17. United States Pharmacopeia and National Formulary, (January 2000), Rockville, Maryland
18. van der Walt JP (1970) Criteria and methods used in classification. In: Lodder J (ed) The yeasts. North Holland, Amsterdam, pp 34-113
19. Zhang W, Bevins MA, Plantz BA, Smith LA, Meagher MM (2000) Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng 70:1-8
20. Zhang W, Inan M, Meagher MM (2000) Fermentation strategies for recombinant protein expression in the methylotrophic yeast Pichia pastoris. Biotechnol Bioprocess Eng 5:275-287