Evaluation of molecular and immunoenzymatic assays for detecting Enterohemorrhagic E coli in food borne outbreaks;Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias

Revista Medica de Chile

Volumn 130, Issue 6, 2002, Pages 603-609

  Vidal O , Maricel ^a   Carreño C , Mónica ^a   Vidal A , Roberto ^a   Arellano C , Carolina ^a   Solari G , Verónica ^a   Prado J , Valeria ^a  

^a UNIVERSITY OF CHILE   (Chile)

Author keywords

DNA probes; Enzyme linked immunosorbent assay; Escherichia coli infections; Escherichia coli O157

Indexed keywords

CYTOTOXIN; OLIGONUCLEOTIDE;

ARTICLE; CHILE; COMPARATIVE STUDY; ENTEROBACTER INFECTION; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIDEMIC; ESCHERICHIA COLI O157; FOOD POISONING; GASTROENTERITIS; GENETIC PROCEDURES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

BASE SEQUENCE; CHILE; CYTOTOXINS; DISEASE OUTBREAKS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI O157; FOOD POISONING; GASTROENTERITIS; GENETIC TECHNIQUES; HUMANS; IMMUNOENZYME TECHNIQUES; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0346678521     PISSN: 00349887     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. HAYES P. Microbiología e higiene de los alimentos. En: Frazier Microbiología de los alimentos. Zaragoza: Editorial Acribia 1993; 23-68.
2. PRADO V, MARTÍNEZ J, ARELLANO C, LEVINE MM. Variación temporal de genotipos y serogrupos de E coli enterohemorrágico aislados en ninos chilenos con infección intestinal o Síndrome Hemolítico Urémico. Rev Méd Chile 1997; 125: 291-7.
3. ORR P, LORENCZ B, BROWN R, KIELLY R, TAN B, HOLTON D ET AL. An outbreak of diarrhea due to Verotoxin producing Escherichia coli in the Canadian Northwest territories. Scand J Infect Dis 1994; 26: 675-8.
4. SAMADPOUR M, ONGERTH J, LISTON J, TRAN N, NGUYEN D, WHITTAM T ET AL. Ocurrence of Shiga-Like-Toxin producing Escherichia coli in retail fresh seafood, beef, lamb, pork and poultry from grocery stores in Seattle, Washington. Appl Environ Microbiol 1994; 60: 1038-40.
5. TESH V. Virulence of enterohemorrhagic Escherichia coli: role of molecular crosstalk. TIMPS 1998; 6: 228-33.
6. JARVIS KG, KAPER JB. Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system. Infect Immun 1996; 64: 4826-9.
7. TESH VL, O'BRIEN AD. The pathogenic mechanisms of Shiga toxin and the Shiga-like toxins. Mol Microbiol 1991; 5: 1817-22.
8. NATARO JP, KAPER JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev 1998; 11: 142-201.
9. VIZCAYA MC, SANDOVAL C, SALÍN MP, PRADO V. Impacto del síndrome hemolítico urémico en las distintas areas de salud de la Región Metropolitana. Rev Chil Infect 1996; 13: 223-30.
10. CEBULA TA, PAYNE WL, FENG P. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. J Clin Microbiol 1995; 33: 248-50.
11. PARK CH, VANDEL NM, HIXON DL. Rapid immunoassay for detection of Escherichia coli O157 directly from stool specimens. J Clin Microbiol 1996; 34: 988-90.
12. MAURER JJ, SCHMIDT D, PETROSKO P, SÁNCHEZ S, BOLTON L, LEE M. Development of primers to O-antigen biosynthesis genes for specific detection of Escherichia coli O157 by PCR. Appl Environ Microbiol 1999; 65: 2954-60.
13. th edition. Difco Laboratories Detroit, Michigan, US.
14. GICQUELAIS K, BALDINI M, MARTÍNEZ J, MAGGI L, MARTIN W, PRADO V ET AL. Practical and Economical Method for Using Biotinylated DNA Probes with Bacterial Colony Blots to Identify Diarrhea-Causing Escherichia coli. J Clin Microbiol 1990; 28: 2485-90.
15. LIN Z, KARAZONO H, YAMAZAKI S, TAKEDA Y. Detection of various variant Verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol Immunol 1993; 37: 543-8.
16. RAMOTAR K, WALDHART B, CHURCH D, SZUMSKI R, LOUIE T. Direct detection of Verotoxin-Producing Escherichia coli in Stool Samples by PCR. J Clin Microbiol 1995; 33: 519-24.
17. KARMALI MA. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 1989; 2: 15-38.