NPAS2: A gas-responsive transcription factor

Science

Volumn 298, Issue 5602, 2002, Pages 2385-2387

  Dioum, Elhadji M ^a   Rutter, Jared ^b   Tuckerman, Jason R ^a   Gonzalez, Gonzalo ^a   Gilles Gonzalez, Marie Alda ^a   McKnight, Steven L ^b  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON MONOXIDE; CONCENTRATION (PROCESS); DIMERIZATION; DIMERS; DNA; GENES; OXIDATION;

PROSTHETIC GROUP;

PROTEINS;

CARBON MONOXIDE; DIMER; HEME; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PROTEIN; PROTEIN BMAL1; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NPAS2; UNCLASSIFIED DRUG;

DNA; GENE;

ARTICLE; CIRCADIAN RHYTHM; CONCENTRATION (PARAMETERS); DIMERIZATION; GAS; GENE EXPRESSION; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; REDUCTION; SENSOR;

ANIMALS; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CARBON MONOXIDE; CIRCADIAN RHYTHM; DIMERIZATION; DNA; HELIX-LOOP-HELIX MOTIFS; HEME; LIGANDS; MYOGLOBIN; NADP; NERVE TISSUE PROTEINS; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; TRANSCRIPTION FACTORS;

MAMMALIA;

EID: 0346668332     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1078456     Document Type: Article

References (31)

1. E. C. Hoffman et al., Science 252, 954 (1991).
2. B. L. Taylor, I. B. Zhulin, Microbiol. Mol. Biol. Rev. 63, 479 (1999).
3. M. A. Gilles-Gonzalez, G. S. Ditta, D. R. Helinski, Nature 350, 170 (1991).
4. W. D. Hoff et al., Biochemistry 33, 139S9 (1994).
5. J. M. Christie, M. Salomon, K. Nozue, M. Wada, W. R. Briggs, Proc. Natl. Acad. Sci. U.S.A. 96, 8779 (1999).
6. S. Hill, S. Austin, T. Eydmann, T. Jones, R. Dixon, Proc. Natl. Acad. Sci. U.S.A. 93, 2143 (1996).
7. M. S. Denison, A. Pandini, S. R. Nagy, E. P. Baldwin, L. Bonati, Chem. Biol. Interact. 146, 3 (2002).
8. U. K. Genick et al., Science 275, 1471 (1997).
9. W. Gong et al., Proc. Natl. Acad. Sci. U.S.A. 95, 15177 (1998).
10. , J. H. Morais-Cabral et al., Cell 95, 649 (1998).
11. S. Crosson, K. Moffat, Proc. Natl. Acad. Sci. U.S.A. 98, 299S (2001).
12. C. A. Amezcua, S. M. Harper, J. Rutter, K. H. Gardner, Structure 10, 1349 (2002).
13. M. A. Gilies-Gonzalez, IUBMB Life 51, 16S (2001).
14. F. Ascoli, M. R. Rossi-Fanelli, E. Antonini, Methods Enzymol. 76, 72 (1981).
15. The insoluble fraction of cell lysates was clarified with 0.5% SDS, which was then removed by KCl precipitation. Get filtration (Sephacryl S-200) and anion-exchange (DEAE-sephacel chromatography yielded ∼90% pure NPAS2 apoprotein. This protein [∼20 μM apo-protein in 0.10 M sodium phosphate, 15% v/v glycerol, and 0.1 mM EDTA (pH 7.5)] was titrated with 2 mM hemin in 10 mM NAOH. The resulting holo-protein was transferred to 0.10 M sodium phosphate, 15% v/v glycerol, and 0.1 mM EDTA (pH 7.5) by get filtration (Sephacryl S-200).
16. M. S. Hargrove et al., J. Biol. Chem. 269, 4207 (1994).
17. C. A. Appleby, in Methods for Evaluating Biological Nitrogen Fixation, F. J. Bergerson, Ed. (Wiley, New York, 1980), pp. 315-335.
18. All three NPAS2 species in this study were truncated forms of the native NPAS-2 protein. They were as follows: bHLH-PAS-A-PAS-B, residues 1 to 416; bHLH-PAS-A, residues 1 to 246; and PAS-B, residues 160 to 346.
19. M.A. Gilles-Gonzalez et al., Biochemistry 33, 8067 (1994).
20. V. M. Delgado-Nixon, G. Gonzalez, M. A. Gilles-Gonzalez, Biochemistry 39, 2685 (2000).
21. D. Shelver, R. L. Kerby, Y. He, G. P. Roberts, Proc. Natl. Acad. Sci. U.S.A. 94, 11216 (1997).
22. G. Gonzalez et al., Biochemistry 41, 8414 (2002).
23. W. N. Lanzitotta et al., Nature Struct. Biol. 7, 876 (2000).
24. M. S. Hargrove, Biophys. J. 79, 2733 (2000).
25. J. Rutter, M. Reick, L. C. Wu, S. L. McKnight, Science 293, 510 (2001).
26. E. M. Dioum et al., data not shown.
27. T. Ingi, G. Chiang, G. V. Ronnett, J. Neurosci. 16, 5621 (1996).
28. M. Reick, J. A. Garcia, C. Dudley, S. L. McKnight, Science 293, 506 (2001).
29. S. R. Vincent, S. Das, M. D. Maines, Neuroscience 63, 223 (1994).
30. B. H. Zheng et al., Cell 105, 683 (2001).
31. We thank M. S. Hargrove and J. S. Olson for H64Y/V68F sperm whale myoglobin and R.W. Estabrook and J. A. Peterson for helpful discussions. Supported by NIH grants R01 HL640381 (M.-A.G.-G.) and NIHS- T32-GM08-291-12 (J.R.), NIMH grant R01 MH5938805 (S.L.M.), and unrestricted funds from an anonymous donor.