Identification and characterization of Lactobacillus helveticus PepO2, an endopeptidase with post-proline specificity

Applied and Environmental Microbiology

Volumn 69, Issue 2, 2003, Pages 1276-1282

  Chen, Yo Shen ^a   Christensen, Jeffrey E ^a,c   Broadbent, Jeffery R ^b   Steele, James L ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UTAH STATE UNIVERSITY   (United States)

^c MARSHFIELD CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CELLS; ENZYME KINETICS; HYDROLYSIS;

NUCLEOTIDE SEQUENCES;

MICROBIOLOGY;

BACTERIAL ENZYME; BETA CASEIN; DNA; PROLINE; PROTEINASE; SYNTHETIC PEPTIDE;

BACTERIUM;

ARTICLE; DNA SEQUENCE; ENZYME ANALYSIS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; LACTOBACILLUS HELVETICUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CASEINS; CLONING, MOLECULAR; ENDOPEPTIDASES; GENOMIC LIBRARY; LACTOBACILLUS; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PROLINE; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); LACTOBACILLUS; LACTOBACILLUS HELVETICUS; LACTOBACILLUS RHAMNOSUS; LACTOCOCCUS; LACTOCOCCUS LACTIS; PEPO; PROKARYOTA;

EID: 0346667192     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.2.1276-1282.2003     Document Type: Article

References (32)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Meyers, and D. J. Lipman, 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Bartels, H. J., M. E. Johnson, and N. F. Olson. 1987. Accelerated ripening of Gouda cheese. 1. Effect of heat-shocked thermophilic lactobacilli and streptococci on proteolysis and flavor development. Milchwissenschaft 42:83-88.
3. Bartels, H. J., M. E. Johnson, and N. F. Olson. 1987. Accelerated ripening of Gouda cheese. 1. Effect of freeze-shocked Lactobacillus helveticus on proteolysis and flavor development. Milchwissenschaft 42:139-144.
4. Broadbent, J. R., M. Barnes, C. Brennand, M. Strickland, K. Houck, M. E. Johnson, and J. L. Steele. 2002. Contribution of Lactococcus lactis cell envelope proteinase specificity to peptide accumulation and bitterness in reduced-fat Cheddar cheese. Appl. Environ. Microbiol. 68:1778-1785.
5. Broadbent, J. R., M. Strickland, B. C. Weimer, M. E. Johnson, and J. L. Steele. 1998. Peptide accumulation and bitterness in Cheddar cheese made using single-strain Lactococcus lactis starters with distinct proteinase specificities. J. Dairy Sci. 81:327-337.
6. Chavagnat, F., J. Meyer, and M. G. Casey. 2000. Purification, characterization, cloning and sequencing of the gene encoding oligopeptidase PepO from Streptococcus thermophilus A. FEMS Microbiol. Lett. 191:79-85.
7. Chen, J.-D., and D. A. Morrison. 1988. Construction and properties of a new insertion vector, pJDC9, that is protected by transcriptional terminators and useful for cloning of DNA from Streptococcus pneamoniae. Gene 64:155-164.
8. Chen, Y.-S., and J. L. Steele. 1998. Genetic characterization and physiological role of endopeptidase O from Lactobacillus helveticus CNRZ32. Appl. Environ. Microbiol. 64:3411-3415.
9. Christensen, J. E., E. G. Dudley, J. A. Pederson, and J. L. Steele. 1999. Peptidases and amino acid catabolism in lactic acid bacteria. Antonie Leeuwenhoek 76:217-246.
10. S1-CN(f1-9) and β-CN(f193-209) by Lactobacillus helveticus peptidase deletion mutants indicates the presence of a previously undetected endopeptidase. Appl. Environ. Microbiol. 69:1283-1286.
11. Christensson, C., H. Bratt, L. J. Collins, T. Coolbear, R. Holland, M. W. Lubbers, P. W. O'Toole, and J. R. Reid. 2002. Cloning and expression of an oligopeptidase, PepO, with novel specificity from Lactobacillus rhamnosus HN001 (DR20). Appl. Environ. Microbiol. 68:254-262.
12. DeMan, J., M. Rogosa, and M. E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23:130-135.
13. Fenster, K. M., K. L. Parkin, and J. L. Steele. 1997. Characterization of a thiol-dependent endopeptidase from Lactobacillus helveticus CNRZ32. J. Bacteriol. 179:2529-2533.
14. Froeliger, E. H., J. Oetjen, J. P. Bond, and P. Fives-Taylor. 1999. Streptococcus parasanguis pepO encodes an endopeptidase with structure and activity similar to those of enzymes that modulate peptide receptor signaling in eukaryotic cells. Infect. Immun. 67:5206-5214.
15. Kaminogawa, S., T. R. Yan, N. Azuma, and K. Yamauchi. 1986. Identification of low molecular weight peptides in Gouda-type cheese and evidence for the formation of these peptides from 23 amino terminal residues of alpha-s1-casein by proteinases of Streptococcus cremoris H61. J. Food Sci. 51:1253-1264.
16. Khalid, N. M., and E. H. Marth. 1990. Purification and partial characterization of a prolyldipeptidyl aminopeptidase from Lactobacillus helveticus CNRZ32. Appl. Environ. Microbiol. 56:381-388.
17. Kunji, E. R. S., I. Mierau, A. Hagting, B. Poolman, and W. N. Konings. 1996. The proteolytic systems of lactic acid bacteria. Antonie Leeuwenhoek 70:187-221.
18. Kok, J., and W. M. De Vos. 1994. The proteolytic system of lactic acid bacteria, p. 169-210. In M. J. Gasson and W. M. de Vos (ed.), Genetics and biotechnology of lactic acid bacteria. Blackie Academic and Professional, Glasgow, United Kingdom.
19. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydrophatic character of a protein. J. Mol. Biol. 157:105-132.
20. Lemieux, L., and R. E. Simard, 1992. Bitter flavor in dairy products. II. A review of bitter peptides from caseins: their formation, isolation and identification, structure masking and inhibition. Lait 72:335-382.
21. Madkor, S. A., P. S. Tong, and M. El Soda. 2000. Ripening of cheddar cheese with added attenuated adjunct cultures of lactobacilli. J. Dairy Sci. 83:1684-1691.
22. Mierau, I., P. S. T. Tan, A. J. Haandrikman, J. Kok, K. J. Leenhouts, W. N. Konings, and G. Venema. 1993. Cloning and sequencing of the gene for a lactococcal endopeptidase, an enzyme with sequence similarity to mammalian enkephalinase. J. Bacteriol. 175:2087-2096.
23. Mulholland, F. 1997. Proteolytic systems of dairy lactic acid bacteria, p. 299-318. In B. A. Law (ed.), Microbiology and biochemistry of cheese and fermented milk. Blackie Academic and Professional, Glasgow, United Kingdom.
24. Nardi, M., P. Renault, and V. Monnet. 1997. Duplication of the pepF gene and shuffling of DNA fragments on the lactose plasmid of Lactococcus lactis. J. Bacteriol. 179:4164-4171.
25. Nowakowski, C. M., T. K. Bhowmik, and J. L. Steele. 1993. Cloning of peptidase genes from Lactobacillus helveticus CNRZ32. Appl. Microbiol. Biotechnol. 39:204-210.
26. Pederson, J. A., G. J. Mileski, B. C. Weimer, and J. L. Steele. 1999. Genetic characterization of a cell envelope-associated proteinase from Lactobacillus helveticus CNRZ32. J. Bacteriol. 181:4592-4597.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
28. Shine, J., and L. Dalgarno. 1974. The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. USA 71:1342-1346.
29. Swaisgood, H. E. 1982. The chemistry of milk protein, p. 1-59. In P. F. Fox (ed.), Developments in dairy chemistry, vol. 1. Elsevier, London, United Kingdom.
30. Terzaghi, B. E., and W. E. Sandine. 1975. Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29:807-813.
31. Tinoco, I. J., P. N. Borer, B. Dengler, M. D. Levine, O. C. Uhlenbeck, and D. M. Crothers. 1973. Improved estimation of secondary structure in ribonucleic acids. Nature (London) New Biol. 246:40-41.
32. Tynkkynen, S., G. Buist, E. Kunji, J. Kok, B. Poolman, G. Venema, and A. Haandrikman. 1993. Genetic and biocemical characterization of the oligopeptide transport system of Lactococcus lactis. J. Bacteriol. 175:7523-7532.