Genetic characterization of a cell envelope-associated proteinase from Lactobacillus helveticus CNRZ32

Journal of Bacteriology

Volumn 181, Issue 15, 1999, Pages 4592-4597

  Pederson, Jeffrey A ^a   Mileski, Gerald J ^b   Weimer, Bart C ^b   Steele, James L ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b UTAH STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CASEIN; PROTEINASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GROWTH; CELL SURFACE; DELETION MUTANT; ENVELOPE GENE; ENZYME ACTIVITY; ENZYME SPECIFICITY; GENETIC CODE; HYDROLYSIS; LACTOBACILLUS HELVETICUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SOUTHERN BLOTTING;

BACTERIA (MICROORGANISMS); LACTOBACILLUS HELVETICUS; POSIBACTERIA; PROKARYOTA;

EID: 0032783298     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.15.4592-4597.1999     Document Type: Article

References (46)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Bhowmik, T., L. Fernandez, and J. L. Steele. 1993. Gene replacement in Lactobacillus helveticus. J. Bacteriol. 175:6341-6344.
3. Boot, H. J., C. P. Kolen, J. M. van Noort, and P. H. Pouwels. 1993. S-layer protein of Lactobacillus acidophilus ATCC 4356: purification, expression in Escherichia coli, and nucleotide sequence of the corresponding gene. J. Bacteriol. 175:6089-6096.
4. Broadbent, J. R., M. Strickland, B. C. Weimer, M. E. Johnson, and J. L. Steele. 1998. Peptide accumulation and bitterness in cheddar cheese using single-strain Lactococcus lactis starters with distinct proteinase specificities. J. Dairy Sci. 81:327-337.
5. Bruinenberg, P. G., P. Doesburg, A. C. Alting, F. A. Exterkate, W. M. de Vos, and R. J. Siezen. 1994. Evidence for a large dispensable segment in the subtilisin-like catalytic domain of the Lactococcus lactis cell-envelope proteinase. Protein Eng. 7:991-996.
6. Chen, Y., and J. L. Steele. 1998. Genetic characterization and physiological role of endopeptidase O from Lactobacillus helveticus CNRZ32. Appl. Environ. Microbiol. 64:3411-3415.
7. Christensen, J. C., and J. L. Steele. Unpublished data.
8. de Vos, W. M., P. Vos, H. de Haard, and I. Boerrigter. 1989. Cloning and expression of the Lactococcus lactis subsp. cremoris SK11 gene encoding an extracellular serine proteinase. Gene 85:169-176.
9. Dudley, E. G., and J. L. Steele. 1994. Nucleotide sequence and distribution of the pepPN gene from Lactobacillus helveticus CNRZ32. FEMS Microbiol. Lett. 119:41-46.
10. Exterkate, F. A., A. C. Alting, and C. J. Slangen. 1991. Specificity of two genetically related cell-envelope proteinases of Lactococcus lactis subsp. cremoris towards alpha s1-casein-(1-23)-fragment. Biochem. J. 273:135-139.
11. Exterkate, F. A., A. C. Alting, and P. G. Bruinenberg. 1993. Diversity of cell envelope proteinase specificity among strains of Lactococcus lactis and its relationship to charge characteristics of the substrate-binding region. Appl. Environ. Microbiol. 59:3640-3647.
12. s1-casein-(1-23)-fragment by the free and bound forms of the cell envelope proteinase of Lactococcus lactis subsp. cremoris under conditions prevailing in cheese. Syst. Appl. Microbiol. 16:1-8.
13. Gilbert, C., D. Atlan, B. Blanc, R. Portailer, J. E. Germond, L. Lapierre, and B. Mollet. 1996. A new cell surface proteinase: sequencing and analysis of the prtB gene from Lactobacillus delbrueckii subsp. bulgaricus. J. Bacteriol. 178: 3059-3065.
14. Gilbert, C., B. Blanc, J. Frot-Coutez, R. Portalier, and D. Allan. 1997. Comparison of cell surface proteinase activities within the Lactobacillus genus. J. Dairy Res. 64:561-571.
15. Holck, A., and H. Naes. 1992. Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobacillus paracasei subsp. paracasei NCDO 151. J. Gen. Microbiol. 138:1353-1364.
16. Jennes, R., and J. Koops. 1962. Preparation and properties of a salt solution which simulates milk ultrafiltrate. Neth. Milk Dairy J. 16:153-164.
17. Juillard, V., H. Laan, E. R. Kunji, C. M. Jeronimus-Stratingh, A. P. Bruins, and W. N. Konings. 1995. The extracellular PI-type proteinase of Lactococcus lactis hydrolyzes beta-casein into more than one hundred different oligopeptides. J. Bacteriol. 177:3472-3478.
18. Kiwaki, M., H. Ikemura, M. Shimizu-Kadota, and A. Hirashima. 1989. Molecular characterization of a cell wall-associated proteinase gene from Streptococcus lactis NCDO763. Mol. Microbiol. 3:359-369.
19. Kojic, M., D. Fira, B. Bojovic, A. Banina, and L. Topisirovic. 1995. Comparative study on cell-envelope associated proteinases in natural isolates of mesophilic lactobacilli. J. Appl. Bacteriol. 79:61-68.
20. Kok, J. 1993. Genetics of proteolytic enzymes of lactococci and their role in cheese flavor development. J. Dairy Sci. 76:2056-2064.
21. Kok, J. 1990. Genetics of the proteolytic system of lactic acid bacteria. FEMS Microbiol. Rev. 87:15-42.
22. Kok, J., K. J. Leenhouts, A. J. Haandrikman, A. M. Ledeboer, and G. Venema. 1988. Nucleotide sequence of the cell wall proteinase gene of Streptococcus cremoris Wg2. Appl. Environ. Microbiol. 54:231-238.
23. Kunji, E. R., I. Mierau, A. Hagting, B. Poolman, and W. N. Konings. 1996. The proteolytic systems of lactic acid bacteria. Antonie Leeuwenhoek 70: 187-221.
24. Law, J., P. Vos, F. Hayes, C. Daly, W. M. de Vos, and G. Fitzgerald. 1992. Cloning and partial sequencing of the proteinase gene complex from Lactococcus lactis subsp. lactis UC317. J. Gen. Microbiol. 138:709-718.
25. Law, J., and A. Haandrikman. 1997. Proteolytic enzymes of lactic acid bacteria. Int. Dairy J. 7:1-11.
26. Martin-Hernandez, M. C., A. C. Alting, and F. A. Exterkate. 1994. Purification and characterization of the mature, membrane-associated cell-envelope proteinase of Lactobacillus helveticus L89. Appl. Microbiol. Biotechnol. 40: 828-834.
27. Mierau, I., E. R. Kunji, G. Venema, and J. Kok. 1997. Casein and peptide degradation in lactic acid bacteria. Biotechnol. Genet. Eng. Rev. 14:279-301.
28. Navarre, W. W., and O. Schneewind. 1994. Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria. Mol. Microbiol. 14:115-121.
29. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1-6.
30. Perona, J. J., and C. S. Craik. 1995. Structural basis of substrate specificity in the serine proteases. Protein Sci. 4:337-360.
31. Russell, A. J., and A. R. Fersht. 1987. Rational modification of enzyme catalysis by engineering surface charge. Nature 328:496-500.
32. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Schneewind, O., D. Mihaylova-Petkov, and P. Model. 1993. Cell wall sorting signals in surface proteins of gram-positive bacteria. EMBO J. 12:4803-4811.
34. Siezen, R. J., and J. A. Leunissen. 1997. Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci. 6:501-523.
35. Siezen, R. J., P. G. Bruinenberg, P. Vos, I. van Alen-Boerrigter, M. Nijhuis, A. C. Alting, F. A. Exterkate, and W. M. de Vos. 1993. Engineering of the substrate-binding region of the subtilisin-like, cell-envelope proteinase of Lactococcus lactis. Protein Eng. 6:927-937.
36. Siezen, R. J., W. M. de Vos, J. A. Leunissen, and B. W. Dijkstra. 1991. Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases. Protein Eng. 4:719-737.
37. Siezen, R. J. 1999. Personal communication.
38. Simonen, M., and I. Palva. 1993. Protein secretion in Bacillus species. Microbiol. Rev. 57:109-137.
39. Stefanitsi, D., and J. R. Garel. 1997. A zinc-dependent proteinase from the cell wall of Lactobacillus delbrueckii subsp. bulgaricus. Lett. Appl. Microbiol. 24:180-184.
40. Stefanitsi, D., G. Sakellaris, and J. R. Garel. 1995. The presence of two proteinases associated with the cell wall of Lactobacillus bulgaricus. FEMS Microbiol. Lett. 128:53-58.
41. Tinococ, I. J., P. N. Borer, B. Dengler, M. D. Levine, O. C. Uhlenbeck, D. M. Crothers, and J. Gralla. 1973. Improved estimation of secondary structure in ribonucleic acids. Nat. New Biol. 246:40-41.
42. Tynkkynen, S., G. Buist, E. Kunji, J. Kok, B. Poolman, G. Venema, and A. Haandrikman. 1993. Genetic and biochemical characterization of the oligopeptide transport system of Lactococcus lactis. J. Bacteriol. 175:7523-7532.
43. Vos, P., I. J. Boerrigter, G. Buist, A. J. Haandrikman, M. Nijhuis, M. B. de Reuver, R. J. Siezen, G. Venema, W. M. de Vos, and J. Kok. 1991. Engineering of the Lactococcus lactis serine proteinase by construction of hybrid enzymes. Protein Eng. 4:479-484.
44. Vos, P., G. Simons, R. J. Siezen, and W. M. de Vos. 1989. Primary structure and organization of the gene for a procaryotic, cell envelope-located serine proteinase. J. Biol. Chem. 264:13579-13585.
45. Wells, J. A., and D. A. Estell. 1988. Subtilisin - an enzyme designed to be engineered. Trends Biochem. Sci. 13:291-297.
46. Xu, F. F., L. E. Pearce, and P. L. Yu. 1990. Molecular cloning and expression of a proteinase gene from Lactococcus lactis subsp. cremoris H2 and construction of a new lactococcal vector pFX1. Arch. Microbiol. 154:99-104.