Localization of the Insertion Site and Pathotype Determination of the Locus of Enterocyte Effacement of Shiga Toxin-Producing Escherichia coli Strains

Applied and Environmental Microbiology

Volumn 70, Issue 1, 2004, Pages 61-68

  Bertin, Yolande ^a   Boukhors, Karima ^a   Livrelli, Valerie ^b   Martin, Christine ^a  

^a CEMAGREF   (France)

^b UNIVERSITÉ CLERMONT AUVERGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; DNA; ELECTROPHORESIS; FOOD PRODUCTS; GENES; HEALTH; RNA;

PATHOGENECITY;

ESCHERICHIA COLI;

DNA FRAGMENT; INTEGRASE; SHIGA TOXIN; TRANSFER RNA;

ARTICLE; BACTERIUM ISOLATION; BINDING SITE; CATTLE; DNA STRUCTURE; EAE BETA GENE; EAE GAMMA GENE; EAE GENE; EAE ZETA GENE; ESCHERICHIA COLI; ESPA GENE; ESPB GENE; GENE; GENE AMPLIFICATION; GENE INSERTION; GENE LOCUS; GENETIC VARIABILITY; INTESTINE CELL; LEE PATHOGENICITY ISLAND; NONHUMAN; NUCLEOTIDE SEQUENCE; PHEU GENE; PHEV GENE; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; RISK ASSESSMENT; SELC GENE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SOUTHERN BLOTTING; STRAIN IDENTIFICATION; TIR GENE;

ADHESINS, BACTERIAL; ALLELES; ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; BLOTTING, SOUTHERN; CARRIER PROTEINS; CATTLE; CHILD; CHROMOSOME MAPPING; ELECTROPHORESIS, GEL, PULSED-FIELD; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; OPERON; SEQUENCE ANALYSIS, DNA; SHIGA TOXINS; VIRULENCE;

BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA;

EID: 0346500448     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.1.61-68.2004     Document Type: Article

References (44)

1. Adu-Bobie, J., G. Frankel, C. Bain, A. G. Goncalves, L. R. Trabulsi, G. Douce, S. Knutton, and G. Dougan. 1998. Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens. J. Clin. Microbiol. 36:662-668.
2. Al-Hasani, K., K. Rajakumar, D. Bulach, R. Robins-Browne, B. Adler, and H. Sakellaris. 2001. Genetic organization of the she pathogenicity island in Shigella flexneri 2a. Microb. Pathog. 30:1-8.
3. An, H., J. M. Fairbrother, J. D. Dubreuil, and J. Harel. 1997. Cloning and characterization of the eae gene from a dog attaching and effacing Escherichia coli strain 4221. FEMS Microbiol. Lett. 148:239-245.
4. An, H., J. M. Fairbrother, J. D. Dubreuil, and J. Harel. 1999. Cloning and characterization of the esp region from a dog attaching and effacing Escherichia coli strain 4221 and detection of EspB protein-binding to HEp-2 cells. FEMS Microbiol. Lett. 174:215-223.
5. Beltrametti, F., A. U. Kresse, and C. A. Guzman. 1999. Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli. J. Bacteriol. 181:3409-3418.
6. Blanc-Potard, A. B., and E. A. Groisman. 1997. The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survival. EMBO J. 16:5376-5385.
7. Caprioli, A., and A. E. Tozzi. 1998. Epidemiology of Shiga toxin-producing Escherichia coli infections in continental Europe, p. 38-48. In J. B. Kaper and A. D. O'Brien (ed.), Eschenchia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington, D.C.
8. China, B., F. Goffaux, V. Pirson, and J. Mainil. 1999. Comparison of eae, tir, espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction. FEMS Microbiol. Lett. 178:177-182.
9. China, B., E. Jacquemin, A. C. Devrin, V. Pirson, and J. Mainil. 1999. Heterogeneity of the eae genes in attaching/effacing Escherichia coli from cattle: comparison with human strains. Res. Microbiol. 150:323-332.
10. Cid, D., J. A. Ruiz-Santa-Quiteria, I. Marin, R. Sanz, J. A. Orden, R. Amils, and R. de la Fuente. 2001. Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids. Microbiology 147:2341-2353.
11. 536) of uropathogenic Escherichia coli strain 536. Infect. Immun. 70:6365-6372.
12. Goffaux, F., B. China, L. Janssen, and J. Mainil. 2000. Genotypic characterization of enteropathogenic Escherichia coli (EPEC) isolated in Belgium from dogs and cats. Res. Microbiol. 151:865-871.
13. Griffin, P. M. 1998. Epidemiology of Shiga toxin-producing Escherichia coli infections in humans in the United States, p. 15-22. In J. B. Kaper and A. D. O'Brien (ed.), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington, D.C.
14. Jores, J., L. Rumer, S. Kiessling, J. B. Kaper, and L. H. Wieler. 2001. A novel locus of enterocyte effacement (LEE) pathogenicity island inserted at pheV in bovine Shiga toxin-producing Escherichia coli strain O103:H2. FEMS Microbiol. Lett. 204:75-79.
15. Kaper, J. B., S. Elliott, V. Sperandino, N. T. Perna, G. F. Mayhew, and F. R. Blattner. 1998. Attaching-and-effacing intestinal histopathology and the locus of enterocyte effacement, p. 163-182. In J. B. Kaper and A. D. O'Brien (ed.), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington D.C.
16. Lalioui, L., and C. Le Bouguenec. 2001. afa-8 gene cluster is carried by a pathogenicity island inserted into tRNA(Phe) of human and bovine pathogenic Escherichia coli isolates. Infect. Immun. 69:937-948.
17. Liu, S. L., A. Hessel, and K. E. Sanderson. 1993. Genomic mapping with I-Ceu I, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria. Proc. Natl. Acad. Sci. USA 90:6874-6878.
18. McDaniel, T. K., K. G. Jarvis, M. S. Donnenberg, and J. B. Kaper. 1995. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA 92:1664-1668.
19. McGraw, E. A., J. Li, R. K. Selander, and T. S. Whittam. 1999. Molecular evolution and mosaic structure of alpha, beta, and gamma intimins of pathogenic Escherichia coli. Mol. Biol. Evol. 16:12-22.
20. Mellies, J. L., S. J. Elliott, V. Sperandio, M. S. Donnenberg, and J. B. Kaper. 1999. The Per regulon of enteropathogenic Escherichia coli: identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)-encoded regulator (Ler). Mol. Microbiol. 33:296-306.
21. Michino, H., K. Araki, S. Minami, T. Nakayama, Y. Ejima, K. Hiroe, H. Tanaka, N. Fujita, S. Usami, M. Yonekawa, K. Sadamoto, S. Takaya, and N. Sakai. 1998. Recent outbreaks of infections caused by Escherichia coli O157:H7 in Japan, p. 73-81. In J. B. Kaper and A. D. O'Brien (ed.), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington, D.C.
22. Moss, J. E., T. J. Cardozo, A. Zychlinsky, and E. A. Groisman. 1999. The selC-associated SHI-2 pathogenicity island of Shigella flexneri. Mol. Microbiol. 33:74-83.
23. Neves, B. C., S. Knutton, L. R. Trabulsi, V. Sperandio, J. B. Kaper, G. Dougan, and G. Frankel. 1998. Molecular and ultrastructural characterisation of EspA from different enteropathogenic Escherichia coli serotypes. FEMS Microbiol. Lett. 169:73-80.
24. Oswald, E., H. Schmidt, S. Morabito, H. Karch, O. Marches, and A. Caprioli. 2000. Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect. Immun. 68:64-71.
25. Paton, J. C., and A. W. Paton. 1998. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin. Microbiol. Rev. 11:450-479.
26. Pradel, N., V. Livrelli, C. De Champs, J. B. Palcoux, A. Reynaud, F. Scheutz, J. Sirot, B. Joly, and C. Forestier. 2000. Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France. J. Clin. Microbiol. 38:1023-1031.
27. Purdy, G. E., and S. M. Payne. 2001. The SHI-3 iron transport island of Shigella boydii 0-1392 carries the genes for aerobactin synthesis and transport. J. Bacteriol. 183:4176-4182.
28. Reid, S. D., C. J. Herbelin, A. C. Bumbaugh, R. K. Selander, and T. S. Whittam. 2000. Parallel evolution of virulence in pathogenic Escherichia coli. Nature 406:64-67.
29. Sambrook, J., and D. W. Russell. 2001. Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor. N.Y.
30. Sanchez-SanMartin, C., V. H. Bustamante, E. Calva, and J. L. Puente. 2001. Transcriptional regulation of the orf19 gene and the tir-cesT-eae operon of enteropathogenic Escherichia coli. J. Bacteriol. 183:2823-2833.
31. Schmidt, H., H. Russmann, and H. Karch. 1993. Virulence determinants in nontoxinogenic Escherichia coli 0157 strains that cause infantile diarrhea. Infect. Immun. 61:4894-4898.
32. Schmidt, H., W. L. Zhang, U. Hemmrich, S. Jelacic, W. Brunder, P. I. Tarr, U. Dobrindt, J. Hacker, and H. Karch. 2001. Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli. Infect. Immun. 69:6863-6873.
33. Smith, H. R., B. Rowe, G. K. Adak, and W. J. Reilly. 1998. Shiga toxin (verotoxin)-producing Escherichia coli in the United Kingdom, p. 49-58. In J. B. Kaper and A. D. O'Brien (ed.), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington, D.C.
34. Sperandio, V., J. B. Kaper, M. R. Bortolini, B. C. Neves, R. Keller, and L. R. Trabulsi. 1998. Characterization of the locus of enterocyte effacement (LEE) in different enteropathogenic Escherichia coli (EPEC) and Shigatoxin producing Escherichia coli (STEC) serotypes. FEMS Microbiol. Lett. 164:133-139.
35. Swenson, D. L., N. O. Bukanov, D. E. Berg, and R. A. Welch. 1996. Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. Infect. Immun. 64:3736-3743.
36. Tarr, C. L., and T. S. Whittam. 2002. Molecular evolution of the intimin gene in O111 clones of pathogenic Escherichia coli. J. Bacteriol. 184:479-487.
37. Tauschek, M., R. A. Strugnell, and R. M. Robins-Browne. 2002. Characterization and evidence of mobilization of the LEE pathogenicity island of rabbit-specific strains of enteropathogenic Escherichia coli. Mol. Microbiol. 44:1533-1550.
38. Verweyen, H. M., H. Karch, F. Allerberger, and L. B. Zimmerhackl. 1999. Enterohemorrhagic Escherichia coli (EHEC) in pediatric hemolytic-uremic syndrome: a prospective study in Germany and Austria. Infection 27:341-347.
39. Vokes, S. A., S. A. Reeves, A. G. Torres, and S. M. Payne. 1999. The aerobactin iron transport system genes in Shigella flexneri are present within a pathogenicity island. Mol. Microbiol. 33:63-73.
40. Welch, R. A., V. Burland, G. Plunkett III, P. Redford, P. Roesch, D. Rasko, E. L. Buckles, S. R. Liou, A. Boutin, J. Hackett, D. Stroud, G. F. Mayhew, D. J. Rose, S. Zhou, D. C. Schwartz, N. T. Perna, H. L. Mobley, M. S. Donnenberg, and F. R. Blattner. 2002. Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli. Proc. Natl. Acad. Sci. USA 99:17020-17024.
41. Whittam, T. S., M. L. Wolfe, I. K. Wachsmuth, F. Orskov, I. Orskov, and R. A. Wilson. 1993. Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea. Infect. Immun. 61:1619-1629.
42. WHO. 1998. Zoonotic non-O157 Shiga toxin-producing Escherichia coli (STEC). Report of a WHO scientific working group meeting. World Health Organization, Geneva, Switzerland.
43. Wieler, L. H., T. K. McDaniel, T. S. Whittam, and J. B. Kaper. 1997. Insertion site of the locus of enterocyte effacement in enteropathogenic and enterohemorrhagic Escherichia coli differs in relation to the clonal phylogeny of the strains. FEMS Microbiol. Lett. 156:49-53.
44. Zhang, W. L., B. Kohler, E. Oswald, L. Beutin, H. Karch, S. Morabito, A. Caprioli, S. Suerbaum, and H. Schmidt. 2002. Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains. J. Clin. Microbiol. 40:4486-4492.