A PCR-RFLP marker distinguishing Ophiostoma clavigerum from morphologically similar Leptographium species associated with bark beetles

Canadian Journal of Botany

Volumn 81, Issue 11, 2003, Pages 1104-1112

  Lee, Sangwon ^a   Kim, Jae Jin ^a   Fung, Simon ^a   Breuil, Colette ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

tubulin gene; Bark beetle; Leptographium; Ophiostoma clavigerum; RFLP

Indexed keywords

BIODIVERSITY; BIOMARKERS; ENZYMES;

POLYMORPHISM;

PLANTS (BOTANY);

BEETLE;

COLEOPTERA; DENDROCTONUS; DENDROCTONUS JEFFREYI; DENDROCTONUS PONDEROSAE; LEPTOGRAPHIUM; MYXOGASTRIA; OPHIOSTOMA; OPHIOSTOMA CLAVIGERUM; SCOLYTINAE;

EID: 0346463032     PISSN: 00084026     EISSN: None     Source Type: Journal    
DOI: 10.1139/b03-101     Document Type: Article

References (29)

1. Ayliffe, M.A., Dodds, P.N., and Lawrence, G.J. 2001. Characterization of a β-tubulin gene from Melampsora lini and comparison of fungal β-tubulin genes. Mycol. Res. 105: 818-826.
2. Barras, S.J., and Perry, T. 1971. Leptographium terabrantis sp. nov. association with Dendroctonus terebrans in loblolly pine. Mycopathol. Mycol. Appl. 43: 1-10.
3. Bernier, L., and Hubbes, M. 1990. Meiotic analysis of induced mutations in Ophiostoma ulmi. Can. J. Bot. 68: 232-235.
4. Cariboo Lumber Manufacturers' Association / Northern Forest Products Association. 2002. Epidemic expansion facts [online]. Mountain Pine Beetle Emergency Task Force. Available from http://www.mountainpinebeetle.com/ 2002_update.htm [accessed 22 November 2002].
5. Davidson, R.W. 1978. Staining fungi associated with Dendroctonus adjunctus in pines. Mycologia, 70: 35-40.
6. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39: 783-791.
7. Harrington, T.C. 1988. Leptographium species, their distributions, hosts and insect vectors. In Leptographium root disease on conifers. Edited by T.C. Harrington and F.W. Cobb, Jr. The American Phytopathological Society Press, St. Paul, Minn. pp. 1-39.
8. Harrington, T.C. 1993. Biology and taxonomy of fungi associated with bark beetles. In Beetle-pathogen interactions in conifer forests. Edited by T.D. Schowalter and G.M. Filip. Academic Press, San Diego. pp. 37-51.
9. Hasegawa, M., Kishino, H., and Yano, T. 1985. Dating the human-ape split by a molecular clock of mitochondrial DNA. J. Mol. Evol. 22: 160-174.
10. Hausner, G., Reid, J., and Klassen, G.R. 2000. On the phylogeny of members of Ceratocystis s.s., and Ophiostoma that possess different anamorphic states, with emphasis on the anamorph genus Leptographium, based on partial ribosomal DNA sequences. Can. J. Bot. 78: 903-916.
11. Kim, J.-J., Kim, S.H., Lee, S., and Breuil, C. 2003. Distinguishing Ophiostoma ips and O. montium two bark beetle-associated sapstain fungi. FEMS Microbiol. Lett. 222: 187-192.
12. Kim, S.H., Uzunovic, A., and Breuil, C. 1999. Rapid detection of Ophiostoma ṗiceae and O. quercus in stained wood using PCR. Appl. Environ. Microbiol. 65: 287-290.
13. Lagerberg, T., Lundberg, G., and Melin, E. 1927. Biological and practical researches into blueing in pine and spruce. Sven. Skogsvardsforen. Tidskr. 25: 145-272.
14. O'Donnell, K., and Cigelnik, E. 1997. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol. Phylogenet. Evol. 7: 103-116.
15. Paine, T.D., Raffa, K.F., and Harrington, T.C. 1997. Interactions among Scolytidae bark beetles, their associated fungi, and live host conifers. Annu. Rev. Entomol. 42: 179-206.
16. Robinson-Jeffrey, R.C., and Davidson, R.W. 1968. Three new Europhium species with Verticicladiella imperfect states on blue-stained pine. Can. J. Bot. 46: 1523-1527.
17. Saitou, N., and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425
18. Six, D.L., and Paine, T.D. 1997a. Leptographium pyrinum is a mycangial of Dendroctonus adjunctus. Mycologia, 88: 739-744.
19. Six, D.L., and Paine, T.D. 1997b. Ophiostoma clavigerum is the mycangial fungus of the Jeffrey pine beetle, Dendroctonus jeffreyi. Mycologia, 89: 858-866.
20. Six, D.L., and Paine, T.D. 1998. Effects of mycangial fungi and host tree species on progeny survival and emergence of Dendroctonus ponderosae (Coleoptera: Scolytidae). Environ. Entomol. 27: 1393-1401.
21. Six, D.L., Harrington, T.C., Steimel, J., McNew, D., and Paine, T.D. 2003. Genetic relationships among Leptographium terebrantis and the mycangial fungi of three western Dendroctonus bark beetles. Mycologia. 95: 781-792.
22. Stobel, G.A., and Sugawara, F. 1986. The pathogenicity of Ceratocystis montia to lodgepole pine. Can. J. Bot. 64: 113-116.
23. Swofford, D.L. 2001. PAUP: phylogenetic analysis using parsimony. Version 4.0b10. Sinauer Associates, Inc., Sunderland, Mass.
24. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. 1997. The ClustalX windows interface: fexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24: 4876-4882.
25. Tsuneda, A., and Hiratsuka, Y. 1984. Sympodial and annellidic conidiation in Ceratocystis clavigera. Can. J. Bot. 62: 2618-2624.
26. Upadhyay, H.P. 1981. A monograph of Ceratocystis and Ceratocystiopsis. The University of Georgia Press, Athens, Ga.
27. Wagner, T.L., Gagne, J.A., Doraiswamy, P.C., Coulson, R.N., and Brown, K.W. 1979. Development time and mortality of Dendroctonus frontalis in relation to changes in tree moisture and xylem water potential. Environ. Entomol. 8: 1129-1138.
28. Whitney, H.S., and Farris, S.H. 1970. Maxillary mycangium in the Mountain Pine Beetle. Science (Washington, D.C.), 167: 54-55.
29. Yamaoka, Y., Hiratsuka, Y., and Maruyama, P.J. 1995. The ability of Ophiostoma clavigerum to kill mature lodgepole-pine trees. Eur. J. For. Pathol. 25: 401-404.